Cytological Techniques

Plant Chromosome Preparations and Staining for Light Microscopic Studies

“…The most striking feature of acetic acid is its swelling effect on cellular structure and tissues fixed in it are soft unlike fixed in alcohol. When combined with alcohol it offsets the shrinkage caused by alcohol [58]. This is a good solvent for aniline dyes and one can easily prepare aceto-carmine or acetoorcein.…”

“…Alcohol replaces the water molecules in the tissue. Alcohol denatures proteins and precipitates nucleic acids [58]. Ethanol as a single fluid is not used as a fixative.…”

Knowledge of Nigerian laboratory technologists and mortuary attendants on various methods of embalming techniques

“…Many techniques for preservation of cadavers have evolved (Saeed et al, 2001). Today, teaching and studying of anatomical sciences are aided by the quality of embalming services (Barker and Silverton, 1998). Embalming services also help in medico-legal activities, provide financial justification and create an opportunity for loved ones to see the deceased before burial (Acherson, 1983).…”

Loss of 14C activity after chemical fixation of phytoplankton: Error source for autoradiography and other productivity measurements 1

“…However, we are unaware of any assurances or experiments in the literature that indicate general retention of most cellular compounds after treatment with preservatives. On the contrary, evidence indicates that no preservative retains all compounds (Baker 1966;Hayat 1970), and a number of workers have documented loss of particular families of compounds due to preservatives (reviews in Pearse 1968;Stoward 1973;Rogers 1973).…”

“…aid retention of structural features through subsequent treatment-as in embedding, etc.). The compounds most widely used are successful because of their ability to bind and stabilize structural proteins (Baker 1966;Pearse 1968;Hayat 1970), thus rendering the cells lifelike for microscopy. However, we are unaware of any assurances or experiments in the literature that indicate general retention of most cellular compounds after treatment with preservatives.…”