Cytological study of DNA content and nuclear morphometric analysis for aid in the diagnosis of high-grade dysplasia within oral leukoplakia

Yang, Xi; Xiao, Xuan; Wu, Wenyan; Shen, Xuemin; Zhou, Zengtong; Shi, Linjun

doi:10.1016/j.oooo.2017.05.509

Cited by 18 publications

(17 citation statements)

References 24 publications

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“…Another study evaluating a similar DNA image cytometry system CytoSavant reported high specificity (96.9 ± 0.6%) but moderate sensitivity (54.4 ± 7%) . In this study, we evaluated the performance of MotiSavant, an automatic DNA cytometry system in detecting cervical cancer and precursor cells in comparison to HPV test.…”

Section: Discussionmentioning

confidence: 99%

“…DNA ploidy evaluation by flow cytometry and DNA image cytometry has been explored as a screening test for cervical cancer . MotiSavant (Motic, Xiamen, China), one of the more commonly used automatic imaging system, is composed of a scanning microscope, a camera and two software: MotiCytometer1.1 for image acquisition and MotiClassify for scoring the images of nuclei . Neoplastic changes were judged based on three criteria: number of aneuploid cell, number of proliferating cells, and presence or absence of aneuploidy peak in the histogram (when the number of cells is plotted against DNA content) and scatter diagram (when nuclear area is plotted against DNA index; Figure )…”

Section: Introductionmentioning

confidence: 99%

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An automated quantitative DNA image cytometry system detects abnormal cells in cervical cytology with high sensitivity

Wong

Tsun

et al. 2018

Cytopathology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An automated quantitative DNA image cytometry system detects abnormal cells in cervical cytology with high sensitivity

Wong

Tsun

et al. 2018

Cytopathology

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“…Aneuploidy can now be measured by using image cytometry, and its application to cytology samples may soon result in rapid and reliable noninvasive tests for risk assessment of oral lesions. 75…”

Section: Biomarkers As Predictors Of Progressionmentioning

confidence: 99%

Oral potentially malignant disorders: risk of progression to malignancy

Speight

Khurram

Kujan

2018

Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

389

388

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Oral potentially malignant disorders (OPMDs) have a statistically increased risk of progressing to cancer, but the risk varies according to a range of patient- or lesion-related factors. It is difficult to predict the risk of progression in any individual patient, and the clinician must make a judgment based on assessment of each case. The most commonly encountered OPMD is leukoplakia, but others, including lichen planus, oral submucous fibrosis, and erythroplakia, may also be seen. Factors associated with an increased risk of malignant transformation include sex; site and type of lesion; habits, such as smoking and alcohol consumption; and the presence of epithelial dysplasia on histologic examination. In this review, we attempt to identify important risk factors and present a simple algorithm that can be used as a guide for risk assessment at each stage of the clinical evaluation of a patient.

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“…It was previously reported that DNA-ICM added in the diagnosis of high-grade dysplasia and staging of oral leukoplakia in the small case series. 11,12 In view of the aforementioned limitations, the aim of this study, thus, was to explore the optimal cut-off values of DNA content and evaluate the diagnostic accuracy of DNA-ICM using brushings for detecting dysplasia and/or carcinoma in a large prospective series of OPMD patients. Notably, we further tested whether DNA aneuploidy was an independent discriminator for dysplasia and carcinoma combined with risk factors by logistic regression in the study.…”

Section: Introductionmentioning

confidence: 99%

DNA aneuploidy with image cytometry for detecting dysplasia and carcinoma in oral potentially malignant disorders: A prospective diagnostic study

Deng

et al. 2020

Cancer Medicine

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Background Current evidence on diagnostic value of aneuploidy with DNA image cytometry (ICM) using brushings for oral potentially malignant disorders (OPMD) is limited by sample size and inconsistent classification criteria of aneuploidy. This study aimed to explore the optimal cut‐off values of DNA content and evaluate the diagnostic accuracy of DNA‐ICM for detecting dysplasia and/or carcinoma in OPMD. Materials and Methods A total of 401 consecutive patients with OPMD were enrolled in this prospective diagnostic study. Brushing and biopsy sample form each patient was processed by DNA‐ICM and histological examination respectively. Results When the optimal cut‐off of at least one aneuploid cell with DNA index (DI) ≥2.3, the area under the curves (AUC) was 0.735 and positive predictive value was 92.7% for detecting dysplasia within OPMD. When the optimal cut‐off of at least one aneuploid cell with DI ≥ 3.5, the AUC was 0.851 and negative predictive values was 96.8% for detecting carcinoma within OPMD. Importantly, multivariate analysis revealed that aneuploidy with DI ≥ 2.3 in OPMD was significantly associated with dysplasia risk (adjusted OR, 5.52; 95%CI, 2.90‐10.51; P < .001), and aneuploidy with DI ≥ 3.5 in OPMD was strongly associated with malignant risk (adjusted OR, 21.05; 95%CI, 9.34‐47.41; P < .001). Conclusions This largest‐scale diagnostic study optimized the criteria of aneuploidy cytology for noninvasive detection of oral dysplasia and carcinoma within OPMD. DNA aneuploidy in OPMD was an independent marker that strongly associated with oral dysplasia/carcinoma. Our findings suggest that DNA‐ICM may serve as a useful noninvasive adjunctive tool for oral cancer and OPMD screening.

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Cytological study of DNA content and nuclear morphometric analysis for aid in the diagnosis of high-grade dysplasia within oral leukoplakia

Cited by 18 publications

References 24 publications

An automated quantitative DNA image cytometry system detects abnormal cells in cervical cytology with high sensitivity

An automated quantitative DNA image cytometry system detects abnormal cells in cervical cytology with high sensitivity

Oral potentially malignant disorders: risk of progression to malignancy

DNA aneuploidy with image cytometry for detecting dysplasia and carcinoma in oral potentially malignant disorders: A prospective diagnostic study

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