Cytological studies on the planarian neoblast

Pedersen, Knud Jørgen

doi:10.1007/bf00342367

Cited by 110 publications

(45 citation statements)

References 13 publications

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“…3A-C). This finding is consistent with the failure to detect ALP activity in planarian neoblasts (Pedersen, 1959). Moreover, ALP activity was not detected in the growth zone where proliferation and differentiation of undifferentiated cells are actively taking place (Fig.…”

Section: Distribution Of Alp Activity In Various Organismssupporting

confidence: 89%

Differential tissue development during embryogenesis and regeneration in an annelid

Myohara

2004

Developmental Dynamics

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The fragmenting potworm Enchytraeus japonensis (Oligochaeta, Annelida) reproduces asexually by dividing the body into several fragments that then regenerate to complete individuals in 4 -5 days. Such large-scale regeneration, however, occurs only in some invertebrates. To better our understanding of why regeneration is so limited in many animals, despite their ability to undergo embryonic development from the single cell of a fertilized egg, comparisons were made between regeneration and embryonic development of E. japonensis by using two methods: histochemistry for alkaline phosphatase (ALP) and immunohistochemistry with an antibody against acetylated tubulin that visualizes nervous system development. The analyses revealed that both ALP expression patterns and central nervous system development differ between embryogenesis and the regeneration, suggesting that regeneration is not a simple reiteration of embryogenesis but involves different regulatory mechanisms. The study provides a basis for the elucidation of mechanisms that are unique and crucial to regeneration.

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Section: Distribution Of Alp Activity In Various Organismssupporting

confidence: 89%

Differential tissue development during embryogenesis and regeneration in an annelid

Myohara

2004

Developmental Dynamics

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“…In addition, extensive studies were performed on the fine structure and ultrastructure of neoblasts, and of adult tissues and regenerating planarians. [12][13][14][15][16][17][18][19] Quantitative cell analysis has been established based on maceration or cell dissociation, and the use of flow cytometry techniques. [20][21][22] Thus, it has been shown that gamma irradiation of planarian results in a loss of the proliferative cells 23 and that neoblasts are undifferentiated cells that are the only cell type capable of undergoing cell division in planarians.…”

Section: Introductionmentioning

confidence: 99%

Autophagy meets planarians

González‐Estévez

2009

Autophagy

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This review aims to demonstrate the importance of freshwater planarians as model organisms, particularly emphasizing those characteristics of the animal that make them a good model to study autophagy. The aim of this review is to provide a better understanding of autophagy in this model for the nonplanarian reader, and elucidate the relevance of autophagy research in this peculiar model organism. Furthermore, I will try to synthesize the evidence showing the importance of autophagy in planarian body remodeling, and I will discuss some ideas about the role of autophagy in stem cell biology. In light of these new developments, it is likely that the planarian field will make an important contribution to the study of the molecular mechanisms involved in autophagy in the future.

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“…Although there have been many histological studies on various species of planarians (Rieger et al ., 1991b and literature therein), most histological studies have been performed to compare taxonomic characteristics between species and there are not so many reports on various tissues of a given planarian species with high regeneration ability. In addition, it is not so easy to distinguish certain tissues from the surrounding tissues in planarians by histochemical methods (for example, Pedersen, 1959). For these reasons, we have used tissue-specific markers to perform histological analyses of the intact (non-regenerating) planarian Dugesia japonica , which was as a standard animal established in our laboratory to study regeneration mechanisms (Orii et al ., 1999).…”

Section: Introductionmentioning

confidence: 99%