Cytological studies in some species of Momordica

Trivedi, Ruchi; Roy, Roshni

doi:10.1007/bf00123635

Cited by 38 publications

(11 citation statements)

References 4 publications

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“…and Roy, 1972) distributed in India, China, Nepal, Bangladesh, Myanmar, Pakistan and Srilanka. Immature green fruits are cooked as vegetable.…”

Section: Abbreviationsmentioning

confidence: 99%

Establishment of an efficient Agrobacterium tumefaciens-mediated leaf disc transformation of spine gourd (Momordica dioica Roxb. ex Willd)

Thiruvengadam¹

2011

Afr. J. Biotechnol.

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Spine gourd (Momordica dioica Roxb. ex Willd) is a medicinally and economically important plant and also used as vegetable. In this study, we established an Agrobacterium tumefaciens-mediated transformation procedure for M. dioica. Leaf explants were incubated with A. tumefaciens strain LBA4404 containing a binary vector pBAL2 carrying the reporter gene β-glucuronidase intron (GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Following co-cultivation, leaf explants were cultured on Murashige and Skoog (MS) + Gamborg et al., (B 5) medium supplemented with 6.6 µM 2,4dichlorophenoxyacetic acid (2,4-D) combined with 3.3 µM 6-benzylaminopurine (BAP) containing 100 mg L-1 kanamycin and 300 mg L-1 carbenicillin. Kanamycin-resistant calluses were induced from the leaf explants after three weeks. Shoot regeneration was achieved after transferring the calluses onto fresh selection medium 8.8 µM BAP and 2.2 µM 2,4-D. Transgenic shoots were excised from callus and elongated in MS medium fortified with 3.0 µM gibberellic acid (GA 3), 100 mg L-1 kanamycin and 300 mg L-1 carbenicillin. Finally, the shoots were rooted on MS basal medium supplemented with 3.0 µM indole 3-butyric acid (IBA) and 100 mg L-1 kanamycin. High transformation frequency was achieved by using three-day-old precultured leaf explants. Furthermore, the presence of acetosyringone (200 µM), infection of explants for 30 min and three days of cultivation proved to be critical factors for greatly improving the transformation efficiency. Incorporation and expression of the transgenes confirmed by polymerase chain reaction (PCR), Southern blot analysis, reverse transcription (RT)-PCR and GUS histochemical assay. Using this protocol, transgenic M. dioica plants can be obtained in approximately three months with a high transformation frequency of 9%.

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“…and Roy, 1972) distributed in India, China, Nepal, Bangladesh, Myanmar, Pakistan and Srilanka. Immature green fruits are cooked as vegetable.…”

Section: Abbreviationsmentioning

confidence: 99%

Establishment of an efficient Agrobacterium tumefaciens-mediated leaf disc transformation of spine gourd (Momordica dioica Roxb. ex Willd)

Thiruvengadam¹

2011

Afr. J. Biotechnol.

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“…); mohodu (Sotho); nkaka (Thonga); etc. The Balsam Pear flowers and fruits throughout the year, but mainly from October to May (Northern Hemisphere) [77]. Tendrils simple, leaves waxy, lower surface paler than upper, deeply palmately 5-7-lobed, to 12 cm long, margin toothed and stalked.…”

Section: The Plantmentioning

confidence: 99%

Momordica balsamina: A Medicinal and Neutraceutical Plant for Health Care Management

Thakur¹,

Bag²,

Sanodiya³

et al. 2009

CPB

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Momordica balsamina, African pumpkin (Cucurbitaceae), is a tendril-bearing, wild climber containing wide spectrum of medicinal and nutritional values and has been used as a traditional folk medicine in many countries. The leaves, fruits, seeds, and bark of the plant contains resins, alkaloids, flavonoids, glycosides, steroids, terpenes, cardiac glycoside, saponins having various medicinal importance viz. anti-HIV, anti-plasmodial, shigellocidal, anti-diarrheal, anti-septic, anti-bacterial, anti-viral, anti-inflammatory, anti-microbial, hypoglycemic, antioxidant, analgesic and hepatoprotective properties. The therapeutic agent 'Momordin' is capable of inhibiting the growth of HIV and other viruses. The leaves are also important source of nutrients having 17 amino acids with adequate mineral composition like potassium, magnesium, phosphorus, calcium, sodium, zinc, manganese and iron. It also helps to combat the problem of micronutrient deficiencies in soil and high value of protein and fat with low fibre content. High potassium content is a good source for the management of hypertension and other cardiovascular conditions. This plant is being promoted as a protein supplement for cereal-based diets in poor rural communities. The commercial exploitation of this plant for biopharmaceuticals and neutraceuticals are some of the prospective future potential of this wild herb. This review discusses the potential of medicinal and nutritional importance of this wild herb for health care management.

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“…(Steussy 1990). Momordica species are not easily amenable to cytological analysis as their chromosomes are very small and do not stain well (Bhaduri and Bose 1947) vis-a-vis the cytoplasm (Trivedi and Roy 1972). In literature, chromosome number of one species of Momordica is ascribed to that of another due to misidentification in taxonomical identity.…”

Section: Introductionmentioning

confidence: 97%

Cytotaxonomical analysis of Momordica L. (Cucurbitaceae) species of Indian occurrence

Bharathi

Munshi

Vinod

et al. 2011

J Genet

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Somatic chromosome number and detailed karyotype analysis were carried out in six Indian Momordica species viz. M. balsamina, M. charantia, M. cochinchinensis, M. dioica, M. sahyadrica and M. cymbalaria (syn. Luffa cymbalaria; a taxon of controversial taxonomic identity). The somatic chromosome number 2n = 22 was reconfirmed in monoecious species (M. balsamina and M. charantia). Out of four dioecious species, the chromosome number was reconfirmed in M. cochinchinensis (2n = 28), M. dioica (2n = 28) and M. subangulata subsp. renigera (2n = 56), while in M. sahyadrica (2n = 28) somatic chromosome number was reported for the first time. A new chromosome number of 2n = 18 was reported in M. cymbalaria against its previous reports of 2n = 16, 22. The karyotype analysis of all the species revealed significant numerical and structural variations of chromosomes. It was possible to distinguish chromosomes of M. cymbalaria from other Momordica species and also between monoecious and dioecious taxa of the genus. Morphology and crossability among the dioecious species was also studied. Evidence from morphology, crossability, pollen viability and chromosome synapsis suggests a segmental allopolyploid origin for M. subangulata subsp. renigera. The taxonomic status of the controversial taxon M. cymbalaria was also discussed using morphological, karyological and crossability data.

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Cytological studies in some species of Momordica

Cited by 38 publications

References 4 publications

Establishment of an efficient Agrobacterium tumefaciens-mediated leaf disc transformation of spine gourd (Momordica dioica Roxb. ex Willd)

Establishment of an efficient Agrobacterium tumefaciens-mediated leaf disc transformation of spine gourd (Momordica dioica Roxb. ex Willd)

Momordica balsamina: A Medicinal and Neutraceutical Plant for Health Care Management

Cytotaxonomical analysis of Momordica L. (Cucurbitaceae) species of Indian occurrence

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