-The fluorescence in situ hybridization (FISH) using two different rDNA probes (18S and 45S) was applied to cucumber (Cucumis sativus L.) chromosomes. After counterstaining with 4'-6-diamidino-2-phenylindole (DAPI), all chromosomes showed visible bands similar to C-banding pattern and could be identified. The 18S and 45S rDNA signals appeared at the pericentromeric regions of chromosomes 1, 2 and 5. After silver staining to identify the nucleolar organizer region (NOR), the mitotic cells had two to four dots in nucleoli at interphase, two bands at the pericentromeric regions of two larger chromosomes at mid-metaphase. Although no NOR-bands appeared clearly at the prometaphase stage, cer tain silver-stained chromosomes were identified. DAPI staining made it possible to identify the silver-stained chromosomes at mid-metaphase and suggested that chromosome 1 or 2 had a silver-stained band at mid-metaphase.