Cytological features of spermatogenesis in Opsariichthys bidens (Teleostei, Cyprinidae)

Tang, Daojun; Gao, Xinming; Lin, Chenwen; Feng, Binbin; Hou, Cong-Cong; Zhu, Junquan; Wang, Jianping

doi:10.1016/j.anireprosci.2020.106608

Cited by 12 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and Bryconops affinis, spermatogenic cysts burst when spermatids occurs [39,41]. Based on our results, the spermatogenesis in O. bidens belong to the cystic type, which is consistent with the report by Tang et al (2020) [35].…”

Section: Testis Development and Spermatogenesis In O Bidenssupporting

confidence: 93%

“…Opsariichthys bidens (Cypriniformes, Cyprinidae) is an important commercial fish in China and aquaculture production is increasing [35]. To develop artificial propagation technology for O. bidens, research on the mechanisms underlying reproduction in the species has attracted substantial attention.…”

Section: Introductionmentioning

confidence: 99%

“…To develop artificial propagation technology for O. bidens, research on the mechanisms underlying reproduction in the species has attracted substantial attention. To date, cytological features of spermatogenesis in O. bidens have been studied by Tang et al (2020) [35]. Spermatogenesis in O. bidens begins with the proliferation of spermatogonia, and after meiotic divisions of spermatocytes, haploid spermatids differentiate into flagellated sperm via morphological changes during spermiogenesis [35].…”

Section: Introductionmentioning

confidence: 99%

“…To date, cytological features of spermatogenesis in O. bidens have been studied by Tang et al (2020) [35]. Spermatogenesis in O. bidens begins with the proliferation of spermatogonia, and after meiotic divisions of spermatocytes, haploid spermatids differentiate into flagellated sperm via morphological changes during spermiogenesis [35]. However, few studies have evaluated the molecular mechanisms of spermatogenesis in O. bidens.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of Mitochondrial Prohibitin in Opsariichthys bidens and Its Potential Functions in Spermatogenesis

Wang

Gao

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Spermatogenesis is the intricate and coordinated process by which spermatogonia develop into haploid differentiated spermatozoa. Mitochondria are essential for spermatogenesis, and prohibitin (PHB) is closely associated with mitochondrial structure and function during spermatogenesis. Although PHB has been implicated in spermatogenesis in some taxa, its roles in Opsariichthys bidens have not been determined. In this study, the expression patterns and potential functions of PHB in spermatogenesis in O. bidens were characterized using histological microscopic observations, PCR cloning, real-time quantitative PCR (qPCR), Western blotting (WB) and immunofluorescence (IF). The full-length cDNA of Ob-phb was 1500 bp encoding 271 amino acids. A sequence alignment demonstrated that the PHB protein is conserved among different animals. qPCR revealed that phb mRNA is widely distributed in O. bidens and highly expressed in the testes at stages IV and V. WB revealed that Ob-PHB is located in the mitochondria of testes. IF revealed the colocalization of PHB signals and mitochondria. Signals were detected around nuclei in spermatogonia and spermatocytes, gradually moving to the tail region during spermiogenesis, and finally aggregating in the midpiece. These results indicate that Ob-PHB was expressed in the mitochondria during spermatogenesis. In addition, this study proposed Ob-PHB may participate in the degradation of mitochondria and cell differentiation during spermatogenesis.

show abstract

Section: Testis Development and Spermatogenesis In O Bidenssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Mitochondrial Prohibitin in Opsariichthys bidens and Its Potential Functions in Spermatogenesis

Wang

Gao

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the desirable texture and flavor of the flesh, O. bidens has relatively high economic values. Artificial breeding of O. bidens began in 2008 ( Jing, 2009 ), and the previous studies focused on the embryonic development ( Jin et al, 2017 ), flesh nutrition content ( Zhang Q. K. et al, 2019 ) and spermatogenesis ( Tang et al, 2020 ) were reported in recent years. Due to the high price, disease resistance, and wide-range temperature adaptation, O. bidens ( Figure 1A ) has become an emerging commercial fish species.…”

Section: Introductionmentioning

confidence: 99%

Chromosome-Level Assembly of the Chinese Hooksnout Carp (Opsariichthys bidens) Genome Using PacBio Sequencing and Hi-C Technology

Guan

Niu

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Transcriptomes of Testes at Different Developmental Stages in the Opsariichthys bidens Predict Key Genes for Testis Development and Spermatogenesis

et al. 2022

View full text Add to dashboard Cite

Cytological features of spermatogenesis in Opsariichthys bidens (Teleostei, Cyprinidae)

Cited by 12 publications

References 32 publications

Characterization of Mitochondrial Prohibitin in Opsariichthys bidens and Its Potential Functions in Spermatogenesis

Characterization of Mitochondrial Prohibitin in Opsariichthys bidens and Its Potential Functions in Spermatogenesis

Chromosome-Level Assembly of the Chinese Hooksnout Carp (Opsariichthys bidens) Genome Using PacBio Sequencing and Hi-C Technology

Transcriptomes of Testes at Different Developmental Stages in the Opsariichthys bidens Predict Key Genes for Testis Development and Spermatogenesis

Contact Info

Product

Resources

About