Cytological endometritis diagnosis in primiparous versus multiparous dairy cows

Druker, Shaked; Sicsic, Ron; Straten, Michael van; Goshen, T.; Kedmi, Maor; Raz, Tal

doi:10.3168/jds.2020-20064

Cited by 6 publications

(5 citation statements)

References 65 publications

(97 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Swab samples were collected using acceptable methods [9, 37]. Before sampling, the vulva was cleaned with a brush and soap water, followed by wipes containing chlorhexidine gluconate and isopropyl alcohol.…”

Section: Methodsmentioning

confidence: 99%

Reproductive Tract Microbial Transitions from Late Gestation to Early Postpartum Using 16S rRNA Metagenetic Profiling in First-Pregnancy Heifers

Druker,

Sicsic,

Ravid

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Studies in recent years indicate that microbial communities residing in the reproductive tract are crucial for shaping mammals’ health and reproductive outcomes. Following parturition, female mammals often exhibit uterine bacterial contamination due to the open cervix allowing microbes from the vaginal microbiota or the environment to enter. This contamination may be associated with postpartum uterine inflammatory diseases, which are more prevalent among primiparous than multiparous individuals, as reported in dairy cows and women. However, investigations into the spatio-temporal microbial transitions in the reproductive tract of primigravid heifers and women remain limited. Our objective was to describe and compare the community compositions in the vagina at late gestation and in the vagina and uterus at early postpartum, in first-pregnancy heifers. Three swab samples were collected from 33 first-pregnancy Holstein-Friesian heifers: vaginal samples at gestation day 258±4, and vaginal and uterine samples at postpartum day 7±2. Each sample underwent 16S rRNA V4 region metagenetic analysis via Illumina MiSeq, with bioinformatics following Mothur MiSeq SOP. Results revealed distinct shifts in microbial composition between the prepartum vagina, postpartum vagina, and postpartum uterus. The late gestation vaginal and early postpartum uterine microbial profiles were the most distinct, with postpartum vaginal samples serving as intermediaries. The postpartum uterus exhibited lower richness and diversity compared to the prepartum and postpartum vagina. Specific microbial markers were associated with anatomical and temporal transitions, indicating a dynamic microbial landscape associated with parturition. The observed differences in phyla and genera across reproductive sites highlight the complexity of these ecosystems, expending avenues for future research in reproductive biology, microbial ecology, and reproductive tract diseases.

show abstract

Section: Methodsmentioning

confidence: 99%

Reproductive Tract Microbial Transitions from Late Gestation to Early Postpartum Using 16S rRNA Metagenetic Profiling in First-Pregnancy Heifers

Druker,

Sicsic,

Ravid

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Several genetic studies of clinical uterine disease have made such distinctions (Zwald et al, 2004;Shabalina et al, 2020;May et al, 2022). Furthermore, a recently published paper suggested that the optimal PMN thresholds and sampling time points were different in the first lactation than in dairy cows in subsequent lactations (Druker et al, 2022).…”

Section: Limitations Of the Studymentioning

confidence: 99%

Heritability of subclinical endometritis in Norwegian Red cows

Diaz-Lundahl

Heringstad

Garmo

et al. 2022

Journal of Dairy Science

View full text Add to dashboard Cite

Subclinical endometritis (SCE) is highly prevalent in dairy cows, causing negative effects on reproductive outcomes and the producer economy. Genetic selection for animals with better resilience against uterine disease should be prioritized due to both sustainability and animal welfare. Therefore, the aim of the present study was to estimate the heritability of SCE in the Norwegian Red (NR) population. Moreover, future perspectives of the condition as a fertility phenotype for breeding are discussed. A total of 1,642 NR cows were sampled for SCE at the time of artificial insemination, using cytotape. The percentage of polymorphonuclear cells (PMN) in each sample was established by cytology, through the counting of 300 PMN and epithelial cells. The mean percentage of PMN was 5%. Different trait definitions were examined, and SCE was defined as binary traits, based on the following cut-off levels of PMN: Cyto0 = PMN >0, Cyto3 = PMN >3%, Cyto5 = PMN >5%, Cyto10 = PMN >10%, and Cyto20 = PMN >20%. The mean ranged from 0.07 (Cyto20) to 0.59 (Cyto0). We also analyzed PMN as a continuous variable using percent PMN. Information on the animals and herds was obtained from the Norwegian Dairy Herd Recording System. The pedigree of cows with data included a total of 24,066 animals. A linear animal model was used to estimate the heritability. The only trait definition that had an estimated genetic variance larger than the standard error was Cyto5, with an estimated heritability of 0.04. For all other definitions, the genetic variance was not significantly different from zero. A cut-off level of 5% PMN has been established as a general threshold for the definition of SCE in earlier literature. The standard errors of the estimated variance components were relatively large, and results should be interpreted with caution. However, the current study indicates that SCE is heritable at a similar level to that of clinical endometritis and metritis, and has potential as a future fertility phenotype to be used for breeding purposes. A more feasible method to diagnose SCE is needed to establish larger data sets.

show abstract

“…In the present study, there was no difference in the ratio of PMNs to epithelial cells in the uterine lumen according to diet in the MP cows. This index is often taken as an indicator of cytological endometritis [41]. Differences according to diet were, however, observed in the mammary gland as the LC cows had a higher SCC and this was associated with a greater proportion of them experiencing clinical mastitis during the first 16 DIM (28.6% vs. 0% in the LC vs. HC groups).…”

Section: Comparison Of the Effects Of The High And Low Concentrate Di...mentioning

confidence: 99%

Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity

Cheng

McLaughlin

Little

et al. 2022

IJMS

View full text Add to dashboard Cite

The functionality of circulating leukocytes in dairy cows is suppressed after calving, with negative energy balance as a risk factor. Leukocyte transcriptomic profiles were compared separately in 44 multiparous (MP) and 18 primiparous (PP) Holstein–Friesian cows receiving diets differing in concentrate proportion to test whether immune dysfunction could be mitigated by appropriate nutrition. After calving, cows were offered either (1) low concentrate (LC); (2) medium concentrate (MC) or (3) high concentrate (HC) diets with proportions of concentrate to grass silage of 30%:70%, 50%:50% and 70%:30%, respectively. Cow phenotype data collected included circulating metabolites, milk yield and health and fertility records. RNA sequencing of circulating leukocytes at 14 days in milk was performed. The HC diet improved energy balance in both age groups. There were more differentially expressed genes in PP than MP cows (460 vs. 173, HC vs. LC comparison) with few overlaps. The MP cows on the LC diet showed upregulation of the complement and coagulation cascade and innate immune defence mechanisms against pathogens and had a trend of more cases of mastitis and poorer fertility. In contrast, the PP cows on the HC diet showed greater immune responses based on both gene expression and phenotypic data and longer interval of calving to conception. The leukocytes of MP and PP cows therefore responded differentially to the diets between age, nutrient supply and immunity affecting their health and subsequent fertility.

show abstract

Cytological endometritis diagnosis in primiparous versus multiparous dairy cows

Cited by 6 publications

References 65 publications

Reproductive Tract Microbial Transitions from Late Gestation to Early Postpartum Using 16S rRNA Metagenetic Profiling in First-Pregnancy Heifers

Reproductive Tract Microbial Transitions from Late Gestation to Early Postpartum Using 16S rRNA Metagenetic Profiling in First-Pregnancy Heifers

Heritability of subclinical endometritis in Norwegian Red cows

Proportion of Concentrate in the Diet of Early Lactation Dairy Cows Has Contrasting Effects on Circulating Leukocyte Global Transcriptomic Profiles, Health and Fertility According to Parity

Contact Info

Product

Resources

About