Cytological Effects of Mutagenic Agents and NaCl on Mitotic Division in Two Iraqi Rice (Oryza sativa L.) Genotypes

Abstract

This study was carried out to determine the effects of NaCl (0.00, 50, 100, 150, 180 or 200) mM and two different types of mutagens chemical mutagen ethyl methyl sulphonate (EMS) at 0.5% and physical mutagen UV-B irradiation (40 min time exposure), on mitotic division in two Iraqi rice genotypes Amber 33 (A33) and Amber Baghdad (AB). Different concentrations of NaCl were used. Mitotic index (MI %) was decreased significantly by increasing NaCl concentration, however the highest mitotic index was recorded in mutated genotypes with EMS and UV-B. Different abnormal chromosomes were noticed in mutated plants. Results showed high percentage of abnormal chromosomes in EMS mutated genotypes. In addition no significant differences between genotypes in mean of mitotic index with respect to all experiments.

Cytogenetic in Vivo Effects of The Aqueous Extract of Raphanus Sativus L. Leaves in Mitosis of the Meristematic Cells of \Onion Roots

“…The percentage of cells undergoing mitosis in each treatment was determined on the basis of a minimum of 1000 cells. This index, along with proportions of abnormal dividing cells , was calculated according to the following equations: %Mitotic Index (MI) = Total number of dividing cells /Total number of cells examined x 100 % phase Index = Total number of phase /Total number of dividing cells x 100 % aberration Index = Total number of aberrant cells /Total number of dividing cells in the same phase x 100 [16,17].…”