Cytological changes in the pars distalis of the female rat hypophysis grafted under the kidney capsule

Aguado, Luis I.; Rodrı́guez, Sara; Bilbao, Alejandro; Rodríguez, Estéban M.

doi:10.1007/bf00236089

Cited by 5 publications

(1 citation statement)

References 12 publications

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“…GH content of mixed islet/pituitary grafts tended to be higher than that of pure islet grafts; however, since serum GH levels were similar in these two groups of recipients, we speculate that ectopic somatotroph cells were actually undergoing atrophy and that if grafts were retrieved a few days later, their GH content would have been more significantly reduced. Conversely, lactotrophs, which are physiologically under central dopaminergic inhibition, survive after renal subcapsular transplantation (30). Accordingly to that, we observed many PRL-positive cells (not shown) and an elevated PRL content in mixed islet/pituitary grafts (Table 2).…”

Section: Discussionsupporting

confidence: 59%

Pituitary cotransplantation significantly improves the performance, insulin content, and vascularization of renal subcapsular islet grafts.

et al. 1999

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Because the pituitary contains hormones with beta-cell trophic activity, we evaluated whether cotransplantation of pituitary tissue with pancreatic islets might be beneficial for islet graft function and survival. Streptozotocin diabetic nude mice were transplanted under the kidney capsule with 150 handpicked islets alone or mixed with two diced pituitaries and were then followed for 4 weeks. Mice transplanted with mixed islet/pituitary grafts had higher levels of circulating prolactin (PRL) than mice transplanted with islets only, while serum cortisol, growth hormone, and follicle-stimulating hormone were similar in the two groups. After transplantation, recipients of mixed islet/pituitary grafts showed a more pronounced decrease in glycemic levels and higher systemic insulin levels than mice transplanted only with islets. Mixed islet/pituitary grafts were macroscopically characterized by an excellent vascularization and were biochemically characterized by higher insulin and PRL content than pure islet grafts. Histologically, posttransplantation remodeling originated a hybrid organ in which healthy, well-vascularized islets were adjacent to pituitary cell clusters. Transplantations performed to address the specific effect of the anterior versus the intermediate pituitary lobes indicated the former as responsible for the improved function of cotransplanted islets. Mixed islet/pituitary grafts composed of anterior lobes were also the best vascularized and were histologically characterized by the presence of many folliculo-stellate cells. In conclusion, we obtained evidence that pituitary cotransplantation significantly improves the function, insulin content, and vascularization of suboptimal islet grafts. Evidence suggesting that ectopically produced PRL and/or locally released angiogenic peptides might play a causal role is provided.

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Section: Discussionsupporting

confidence: 59%

Pituitary cotransplantation significantly improves the performance, insulin content, and vascularization of renal subcapsular islet grafts.

et al. 1999

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Appearance of the cyst‐ or ductule‐like structures and their role in the restoration of the rat pituitary autograft

Gon¹,

Shirasawa²,

Ishikawa³

1987

Anat. Rec.

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The anterior pituitary glands of 31-day-old male rats were autotransplanted under renal capsule. At the 3rd and 5th day after transplantation, the autografts were observed by electron microscopy and by immunohistochemistry using antiserum against S-100 protein, which serves as marker of folliculo-stellate (FS) cells. On the 3rd day, a large of the graft was replaced with loose connective tissue in which the FS cells were remarkable in number. The FS cells encircled a small number of granular cells and formed cyst- or ductule-like structures with neighboring FS cells. Together, FS cells and the encircled granular cells formed a basement membrane surrounding the entire ductule. Throughout the regenerating gland, many such ductules were found. On the 5th day, both granular cells and FS cells increased in number. The above observations suggest that FS cells may play an important role in the restoration of degenerated pituitary glandular tissues during the early stage of the transplantation.

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Functional Autonomy of the Adenohypophysis

Naik

1999

Neural Regulation in the Vertebrate Endocrine System

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Cytological changes in the pars distalis of the female rat hypophysis grafted under the kidney capsule

Cited by 5 publications

References 12 publications

Pituitary cotransplantation significantly improves the performance, insulin content, and vascularization of renal subcapsular islet grafts.

Pituitary cotransplantation significantly improves the performance, insulin content, and vascularization of renal subcapsular islet grafts.

Appearance of the cyst‐ or ductule‐like structures and their role in the restoration of the rat pituitary autograft

Functional Autonomy of the Adenohypophysis

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