Cytological Aspects of Antimicrobial Antibiosis Iii

Kaye, Jeremy J.; Chapman, George B.

doi:10.1128/jb.86.3.536-543.1963

Cited by 30 publications

(2 citation statements)

References 19 publications

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“…It has been reported that Acinetobacter are short rods in the logarithmic phase of growth but often become more coccoid in the stationary phase 26 . In this study, the short bacilli tended to be more damaged than the long bacilli, suggesting that the growth phase may be related to the onset of initial damage caused by colistin 27 , but further study is needed.…”

Section: Discussionmentioning

confidence: 78%

Detection of antimicrobial impact on gram-negative bacterial cell envelope based on single-cell imaging by scanning electron microscopy

Hisada

Matsumoto

Hirano

et al. 2023

Sci Rep

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Rapid determination of drug efficacy against bacterial pathogens is needed to detect potentially resistant bacteria and allow for more rational use of antimicrobials. As an indicator of the antimicrobial effect for rapid detection, we found changes in image brightness in antimicrobial-affected bacteria by scanning electron microscopy (SEM). The cell envelopes of unaffected bacteria were stained with phosphotungstic acid (PTA), whereas the entire cells of affected bacteria were stained. Since tungsten density increases backscattered electron intensity, brighter bacterial images indicate lethal damage. We propose a simplified method for determining antimicrobial efficacy by detecting damage that occurs immediately after drug administration using tabletop SEM. This method enabled the visualization of microscopic deformations while distinguishing bacterial-cell-envelope damage on gram-negative bacteria due to image-brightness change. Escherichia coli, Acinetobacter baumannii, Enterobacter cloacae, Klebsiella pneumoniae, and Pseudomonas aeruginosa were exposed to imipenem and colistin, which affect the cell envelope through different mechanisms. Classification of single-cell images based on brightness was quantified for approximately 500 bacteria per sample, and the bright images predominated within 5 to 60 min of antimicrobial treatment, depending on the species. Using intracellular PTA staining and characteristic deformations as indicators, it was possible to determine the efficacy of antimicrobials in causing bacterial-cell-envelope damage.

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Section: Discussionmentioning

confidence: 78%

Detection of antimicrobial impact on gram-negative bacterial cell envelope based on single-cell imaging by scanning electron microscopy

Hisada

Matsumoto

Hirano

et al. 2023

Sci Rep

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“…4:671, 1958. ) investigation, however, it is now apparent that the distinction is not completely valid, for mesosomes have been described even in E. coli (45,46). It seems, nevertheless, that they are rare under normal conditions of growth in enterobacteria, whereas they are abundant and readily demonstrated in many gram-positive microorganisms, including streptococci (Fig.…”

Section: Speculationmentioning

confidence: 99%

Symposium on the fine structure and replication of bacteria and their parts. 3. Bacterial cell-wall replication followed by immunofluorescence.

Cole¹

1965

Bacteriological Reviews

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Cytogenetic effect of colistin on human lymphocytes in vitro: Chromosome aberrations, sister chromatid exchanges, mitotic index, cell cycle kinetics, and acrocentric associations

Jaju¹,

Ahuja²

1983

Teratog. Carcinog. Mutagen.

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Colistin, a peptide antibiotic, was tested at three different concentrations--71, 142, and 214 units/ml. One hundred forty-two units per milliliter corresponds to the plasma level after receiving therapeutic dose. There was a dose-dependent increase in the frequency of chromosome aberrations irrespective of the duration of treatment (T0, T24, T48). This antibiotic decreased the mitotic index and delayed the cell turn over rate indicating inhibition of DNA synthesis by it. Inhibition of DNA synthesis probably results in increase in the frequency of chromosome aberrations. Frequency of satellite associations of acrocentric chromosomes was increased with increasing concentration of the drug, but the differences at three concentrations were not significant compared to controls. There was no increase in the frequency of SCEs at any concentration or duration of treatment compared to controls. It appears that colistin induces the type of lesions that lead to chromosome aberrations and not to SCEs.

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Cytological Aspects of Antimicrobial Antibiosis Iii

Cited by 30 publications

References 19 publications

Detection of antimicrobial impact on gram-negative bacterial cell envelope based on single-cell imaging by scanning electron microscopy

Detection of antimicrobial impact on gram-negative bacterial cell envelope based on single-cell imaging by scanning electron microscopy

Symposium on the fine structure and replication of bacteria and their parts. 3. Bacterial cell-wall replication followed by immunofluorescence.

Cytogenetic effect of colistin on human lymphocytes in vitro: Chromosome aberrations, sister chromatid exchanges, mitotic index, cell cycle kinetics, and acrocentric associations

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