Cytological and physiological behaviour of <i>Euglena gracillis</i> cells entrapped in a calcium alginate gel

Tamponnet, C.; Coslantino, François.; Barbotin, Jean‐Noël; Calvayrac, Régis

doi:10.1111/j.1399-3054.1985.tb04265.x

Cited by 41 publications

(14 citation statements)

References 14 publications

(4 reference statements)

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“…Such interpretation is extended to the Introduction Very little is known about the mechanisms inducing changes in the metabolic behaviour of immobilized cells (Mattiasson et al 1984). The alginate gelification that is commonly used for the immobilization of photosynthetic cells (Schnabl et al 1983;Robinson et al 1985;Tamponnet et al 1985;Papageorgiou et al 1988) requires the presence of divalent cations at a concentration of 1 0 0 m M ; C a 2+ exhibits the best preservation (Kierstan and Bucke 1977). Previous studies (Tamponnet et al 1985) that have demonstrated the arrest and stabilization of the cellular characteristics of Euolena 9racilis Z cells immobilized by Ca 2+ alginate and stored in darkness at 4 ° C have also suggested an optimal role for the external calcium as compared to other divalent cations.…”

Section: Discussionmentioning

confidence: 99%

Physiological stabilization of Euglena gracilis cells by high extracellular calcium (100 mM)

Tamponnet

Barbotin

Calvayrac³

1989

Appl Microbiol Biotechnol

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Experiments were performed to test whether or not high concentrations of CaC12 (100 mM) are able to arrest and stabilize internal structures and associated functions in EutTlena 9racilis Z cells stored in darkness at 4 ° C. Storage of photoheterotrophically grown green cells in high Ca 2+ media (2-100 mM) retards pheophytinization of the chlorophylls, preserves photosynthetic activities and stabilizes the structural organization of the associated light-harvesting complexes of the photosystem II units. Alterations of photosynthesis and respiration by chlorpromazine or by temperature are strongly reduced in cells stored under such conditions. More precisely, a chlorpromazine inhibition site is evidenced in the mitochondrial electron pathway and its location in the chloroplastic electron pathway is clarified.Adaptation of Euglena cells from 2 m M to 100 m M Ca 2+ medium is accompanied by an increase both in the externally bound and total internal calcium concentration. A mechanism involving a Ca 2+ deposit on internal membranes is proposed. Such interpretation is extended to the level of chlorophyll fluorescence with DCMU; Fmax, maximal level of chlorophyll fluorescence with DCMU; Fo, level of chlorophyll fluorescence after transients; Ft, level of chlorophyll fluorescence with DCMU; Pheo, pheophytins; P S I and PS II, photosystems I and II; SMi, storage medium Offprint requests to: C. Tamponnet

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Section: Discussionmentioning

confidence: 99%

Physiological stabilization of Euglena gracilis cells by high extracellular calcium (100 mM)

Tamponnet

Barbotin

Calvayrac³

1989

Appl Microbiol Biotechnol

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“…The supernatant was removed and fresh fixative added to the pellet of cercariae, which were left at 4°C for 1 hr. The suspension was centrifuged as before, the supernatant removed and the cercariae were concentrated into alginate bead matrices using a method adapted from Kierstan & Bucke (1977) and Tamponnet et al (1985). The cercariae were washed twice in 0.1 M cacodylate buffer (5 min each), spun again, the supernatant removed and 0.5 ml of 5% aqueous sodium alginate added.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructure of the digestive system of the cercaria of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae)

1994

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The digenean blood fluke Sanguinicola inermis and the family Sanguinicolidae have previously been described as apharyngeate and sucker-less. Examination of the digestive tract of the cercaria of S. inermis with transmission electron microscopy (TEM), however, has confirmed the presence of a well-developed and complex muscular region immediately adjacent to the sub-terminal mouth. The presence of this muscular region, which may be an oral sucker, emphasises the need for further ultrastructural studies on other members of the family. The adjacent oesophagus, composed of modified tegument, has distinct anterior and posterior regions. TEM shows that the surface of the posterior oesophagus is deeply folded and the apical cytoplasm and sunken cytons become filled with secretory granules, eventually forming a "secretory region" adjacent to the intestine. The dorsal, sac-like intestine is composed of a single layer of epithelial cells, the apical surface of which is drawn into shallow folds. Each cell contains numerous secretory granules, rough ER, and a single basal nucleus. The intestinal lumen, which contains electrondense striated bodies, is filled with fibrous material. Only two sensory oral papillae are associated with the digestive tract, positioned either side of the mouth opening and possibly acting as tango-, stretch-or pressure receptors. The papillae comprise several microtubules lying beneath the apical cytoplasm of the tegument within a nerve process, but there is no connection with the external body surface. The morphology of the alimentary canal of the S. inermis cercaria is discussed in relation to the functioning of the digestiv e system in preparation for establishment in the final host.

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“…Physical techniques have been favoured hitherto, such as entrapment in agar (Kayano et al 1981) and in calcium alginate (Musgrave et al 1982(Musgrave et al , 1983Ochiai et al 1983;Robinson et al 1985;Tamponnet et al 1985), and adsorption in porous polyurethane (Gudin and Thomas 1981;Brouers et al 1982;Muallem et al 1983). Although physical immobilization has the advantage of better maintenance of metabolism and may allow cell proliferation, it has a number of disadvantages, such as the biodegradability of biogels, the incompatibility of calcium alginate with phosphate ions, the tendency of the cells to leak out of the matrix and the opaqueness of polyurethane.…”

Section: Introductionmentioning

confidence: 99%

Immobilization of photosynthetically active cyanobacteria in glutaraldehyde-crosslinked albumin matrix

Papageorgiou

Lagoyanni

1986

Appl Microbiol Biotechnol

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Summary. Cryoprotection was afforded to cells ofPhormidium luridum and Anacystis nidulans by preloading them with dimethyl sulphoxide and then performing the immobilization reaction in dimethyl sulphoxide-free medium. Compared with free cyanobacteria cells, matrix-immobilized cyanobacteria exhibit superior temperature tolerance and storage longevity of photoinduced electron transport, while their phycobiliproteins are better protected against thermal denaturation. Photosynthetically active Phormidium spheroplasts capable of interacting with ionic Hill cofactors, were immobilized in crosslinked albumin matrix, with cryoprotection provided by sorbitol.

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Cytological and physiological behaviour of Euglena gracillis cells entrapped in a calcium alginate gel

Cited by 41 publications

References 14 publications

Physiological stabilization of Euglena gracilis cells by high extracellular calcium (100 mM)

Physiological stabilization of Euglena gracilis cells by high extracellular calcium (100 mM)

Ultrastructure of the digestive system of the cercaria of Sanguinicola inermis Plehn, 1905 (Digenea: Sanguinicolidae)

Immobilization of photosynthetically active cyanobacteria in glutaraldehyde-crosslinked albumin matrix

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