Cytological and morphology characteristics of natural microsporogenesis within Camellia oleifera

Zhang, Xiaoyu; Tong, Hailang; Han, Zhiqiang; Huang, Long; Tian, Jichun; Fu, Zhixing; Wu, Yunyi; Wang, Ting; Yuan, Deyi

doi:10.1007/s12298-021-01002-5

Cited by 6 publications

(7 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flow cytometry has been widely used to measure pollen nuclear DNA content in order to understand pollen development and detect the presence of 2 n pollen ( Bino et al, 1990 ; Dewitte et al, 2009 ; van Laere et al, 2009 ; Zhu et al, 2014 ; Zhang et al, 2021 ). Estimating male 2 n gametes with flow cytometry entails extracting nuclei from a large number of pollen grains, staining them with a DNA-selective fluorochrome, and generating fluorescence histograms with peaks corresponding to groups of nuclei with different DNA content.…”

Section: Methods For Identification Of 2 N Gametesmentioning

confidence: 99%

“…The occurrence in 2 n pollen is associated with the presence of monads, dyads, or triads during microsporogenesis ( Negri et al, 1995 ; Fernandez et al, 2010 ; Chung et al, 2013 ; Nakato and Masuyama, 2021 ; Xu et al, 2021 ; Zhang et al, 2021 ) except 2 n gamete formation that is the result of pre- or post-meiotic restitution. Analysis of microsporogenesis may therefore provide an alternative method to identify 2 n pollen, but this method does not provide any information about pollen viability.…”

Section: Methods For Identification Of 2 N Gametesmentioning

confidence: 99%

“…The use of molecular cytological techniques, such as GISH and fluorescent in situ hybridization in combination with marker analysis, such as amplified fragment length polymorphism on meiocytes or polyploid progeny provides more accurate or additional information on the mechanisms behind 2 n gamete formation ( Barba-Gonzalez et al, 2005 ; Chung et al, 2013 ; Zhang et al, 2021 ). Molecular cytological approaches have been successfully used in the case of allopolyploids, where the constituent genomes can be clearly discriminated ( Xi et al, 2015 ).…”

Section: Methods For Identification Of 2 N Gametesmentioning

confidence: 99%

See 2 more Smart Citations

Exploiting Unreduced Gametes for Improving Ornamental Plants

Xie

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

The formation of gametes with somatic chromosome number or unreduced gametes (2n gametes) is an important process involved in the origin of polyploid plants in nature. Unreduced gametes are the result of meiotic mutations occurring during micro- and mega-sporogenesis. 2n gametes have been identified or artificially induced in a large number of plant species. Breeding of plants through 2n gametes can be advantageous because it combines genetic effects of polyploidy with meiotic recombination and sexual hybridization to produce tremendous genetic variation and heterosis. 2n gametes also occur in ornamental plants, but the potential of using 2n gametes in ornamental plant breeding has not been extensively exploited. Ornamental plants are primarily produced for their esthetic appearance and novelty, not for food and yield, and they can be readily propagated through vegetative means. Triploids, tetraploids, and plants with even higher ploidy levels produced through 2n gametes can be propagated through tissue culture to fix their phenotypes, thus leading to the development of new cultivars. In this review article, we intend to discuss the mechanisms underlying the formation of 2n gametes, techniques for 2n gamete identification, methods for enhancing 2n gamete formation, and the current status in the use of 2n gametes for development of novel ornamental plants. We believe that polyploidy breeding through 2n gametes represents a viable way of developing new cultivars, new species, and even new genera of ornamental plants.

show abstract

Section: Methods For Identification Of 2 N Gametesmentioning

confidence: 99%

Section: Methods For Identification Of 2 N Gametesmentioning

confidence: 99%

Section: Methods For Identification Of 2 N Gametesmentioning

confidence: 99%

See 1 more Smart Citation

Exploiting Unreduced Gametes for Improving Ornamental Plants

Xie

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…With a plethora of plant genomes now sequenced, -omics driven technologies are playing a significant role in the study of the regulatory mechanism of anther development [25,26]. Still, the identification and functional exploration of C. oleifera molecular regulatory network during anther development had been very limited, as previous investigations were mainly focused on cytological studies and single regulatory genes [27][28][29]. In this study, the anther development in the tropical C. oleifera was morphologically and cytologically monitored on temporal scales, and important stages were selected for sequencing, to systematically identify the mRNAs, lncRNAs and miRNAs, and their regulatory network.…”

Section: Transcriptome Mrna In the Anther Development Of The Tropical...mentioning

confidence: 99%

“…Several studies have reported direct correlation of floral bud size and other inflorescence characteristics to anther and microspore development, as it is shown that the development of anther and microspore advances as the size of floral buds increase [30][31][32]. For instance, the meiotic phase of pollen mother cell can be predicted accurately by using the length-breadth ratio of floral bud in the tropical C. oleifera [29]. Our morphological observations and analysis of paraffin sections, combined with previous studies [27,28], also show that significant changes in floral bud lengths reflected the changes in anthers.…”

Section: Transcriptome Mrna In the Anther Development Of The Tropical...mentioning

confidence: 99%

Identification of long non-coding RNAs and microRNAs involved in anther development in the tropical Camellia oleifera

Kong

Zhuo

et al. 2022

BMC Genomics

View full text Add to dashboard Cite

Background Explored the molecular science of anther development is important for improving productivity and overall yield of crops. Although the role of regulatory RNAs, including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), in regulating anther development has been established, their identities and functions in Camellia oleifera, an important industrial crop, have yet not been clearly explored. Here, we report the identification and characterization of genes, lncRNAs and miRNAs during three stages of the tropical C. oleifera anther development by single-molecule real-time sequencing, RNA sequencing and small RNA sequencing, respectively. Results These stages, viz. the pollen mother cells stage, tetrad stage and uninucleate pollen stage, were identified by analyzing paraffin sections of floral buds during rapid expansion periods. A total of 18,393 transcripts, 414 putative lncRNAs and 372 miRNAs were identified, of which 5,324 genes, 115 lncRNAs, and 44 miRNAs were differentially accumulated across three developmental stages. Of these, 44 and 92 genes were predicted be regulated by 37 and 30 differentially accumulated lncRNAs and miRNAs, respectively. Additionally, 42 differentially accumulated lncRNAs were predicted as targets of 27 miRNAs. Gene ontology enrichment indicated that potential target genes of lncRNAs were enriched in photosystem II, regulation of autophagy and carbohydrate phosphatase activity, which are essential for anther development. Functional annotation of genes targeted by miRNAs indicated that they are relevant to transcription and metabolic processes that play important roles in microspore development. An interaction network was built with 2 lncRNAs, 6 miRNAs and 10 mRNAs. Among these, miR396 and miR156 family were up-regulated, while their targets, genes (GROWTH REGULATING FACTORS and SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes) and lncRNAs, were down-regulated. Further, the trans-regulated targets of these lncRNAs, like wall-associated kinase2 and phosphomannose isomerase1, are involved in pollen wall formation during anther development. Conclusions This study unravels lncRNAs, miRNAs and miRNA-lncRNA-mRNA networks involved in development of anthers of the tropical C. oleifera lays a theoretical foundation for further elucidation of regulatory roles of lncRNAs and miRNAs in anther development.

show abstract