Cytological and Morphological Characterization of Pisum sativum and Pisum fulvum Tetraploids

Errico, A.; Conicella, Clara; Taliercio, U.

doi:10.1111/j.1439-0523.1991.tb00492.x

Cited by 4 publications

(3 citation statements)

References 9 publications

(1 reference statement)

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“…The occurrence of polyploidy in the cells (Fig 3E) is associated above all with the endoreduplication process and the course of C-mitosis as indicated by many enlarged nuclei at the initial callus stage (21,20). The number of tetraploids in pea ( Fig 4A) was relatively low and contradictory to most reports showing prevailing tetraploidisation in cultures of this species (64,41,36,14). In most cases, the cultures were subcultivated for many years and this distinction may be the explanation for the discrepancy in the results, than it is apparent tendency from different genotypes of pea to tetraploidisation although the development of this pea culture is not an anomaly.…”

Section: Electron-microscotjycontrasting

confidence: 68%

Initial Stages of Callus Development on Internodal Stem Segments of Pea and Faba BeanIn Vitro

Gantchev¹

1997

Biotechnology & Biotechnological Equipment

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We have investigated the morphology and the cytogenetics in cultivated stem cells of pea (Piswn sativum L.) and faba bean (Vi cia faba L.) at the initial stages of callus development in liquid nutrient medium containing different concentrations 2, 4-D (2,4-dichlorophenoxyacetic acid) ofO, 2, 4, 8, 16 and 32 mgt 1 . The earliest stage of callus development in both species was indicated by the formation of specific morphological patterns -the so-called dividing rows (DR). These arose after periclinal divisions of medium-sized parenchyma cells from the supe1jiciallayers in the neighbourhood of the vascular bundles. DR consist of small meristematic cells with high mitotic activity. At the end of the second week of treatment, their structure was lost and meristematic regions of cells with varying shape and size remain in the actively proliferating tissue. Callus induction and development were obtained in the presence of 2 mgt 1 of 2,4-D only and this process was initiated by the formation of DR. Xylem and cambium were not involved in callus development in both species. That was true for the epidermis tissue in pea too. The faba bean epidermis was integrated in the proliferation process. In cultures lacking 2,4-D no callus was observed. Concentration of32 mgl-1 was found to inhibit the mitosis. The most frequent aberrations in the two species were polyploidy and aneuploidy. Other anomalies were the enlargement of cells and/or nuclei, bi-and multinuclear cells, increased number of nucleoli, unstained nuclei and loosen nuclear structure. The mitotic aberrations included laggard formation, chromatin bridges besides irregular chromatin distribution and chromosomes with decreased integrity in the metaphase. The observed morphological and cytogenetical anomalies have been arisen under the influence of the cultural conditions. Their distribution by the two species suggests different genotype response in vitro. Deep invaginations in the nuclei of dedifferentiated cells and changes in the wall thickness were observed electron microscopically.

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Section: Electron-microscotjycontrasting

confidence: 68%

Initial Stages of Callus Development on Internodal Stem Segments of Pea and Faba BeanIn Vitro

Gantchev¹

1997

Biotechnology & Biotechnological Equipment

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“…The reproductive barriers between wild crop progenitors and domesticated crops might be attributed to several mechanisms, including differences in karyotype or chromosomal rearrangements. Such karyotype differences are reported between P. fulvum and P. elatius , P. sativum , and between P. sativum and P. abyssinicum [72,73], and contribute to the partial fertility of the respective hybrids. Much less is known about the interactions between nuclear and cytoplasmic genomes.…”

Section: Discussionmentioning

confidence: 76%

Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.)

Nováková

Zablatzká

Brus

et al. 2019

IJMS

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Reproductive isolation is an important component of species differentiation. The plastid accD gene coding for the acetyl-CoA carboxylase subunit and the nuclear bccp gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated (Pisum sativum, P. abyssinicum) and wild (P. fulvum and P. elatius) peas. Based on deduced protein sequences, we identified 34 accD and 31 bccp alleles that are partially geographically and genetically structured. The accD is highly variable due to insertions of tandem repeats. P. fulvum and P. abyssinicum have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between P. sativum and P. elatius. Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.

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“…They also reported that the chromosome length and the relative chromosome length varied between 3.86 -5.92 p and 5.64-8.86, respectively. In addition, Kar and Sen (1991), Mannan et al (1991) and Errico et al (1991) obtained that the chromosome number of Pisum sativum L. was 2n= 14. Kar and Sen (1991) reported that the chromosome lengths of Pisum sativum L. varied from 4.5-5.5 p. Kar and Sen (1991) also reported that the chromosomes of Pisum safivum L. have nearly submedian to submedian primary constriction.…”

Section: Introductionmentioning

confidence: 99%

Karyotype Analysis of Pisum arvense L. Collected from Tokat Native Vegetation

Karadağ¹

2003

Tarım Bilimleri Dergisi

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This research was conducted to determine karyotype analysis of Pisum arvense L. occurred in native vegetation of Tokat region. After the seeds were germinated, they were stained by feulgen and karyotype analysis was carried out with obtained cells by using the squash method. The results of the cytological studies that the chromosome numbers of Pisum arvense L. were 2n= 14. Four chromosomes of the Pisum arvense L. were submedian and ten chromosomes were median. In addition, the chromosome length, the arm index and the relative chromosome length in Pisum arvense L. were 4. 59-5.93 p, 0.56-0.91 and 6.38-8.24, respectively.

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Cytological and Morphological Characterization of Pisum sativum and Pisum fulvum Tetraploids

Cited by 4 publications

References 9 publications

Initial Stages of Callus Development on Internodal Stem Segments of Pea and Faba BeanIn Vitro

Initial Stages of Callus Development on Internodal Stem Segments of Pea and Faba BeanIn Vitro

Allelic Diversity of Acetyl Coenzyme A Carboxylase accD/bccp Genes Implicated in Nuclear-Cytoplasmic Conflict in the Wild and Domesticated Pea (Pisum sp.)

Karyotype Analysis of Pisum arvense L. Collected from Tokat Native Vegetation

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