Cytological analysis of bronchoalveolar lavage fluid of dogs with Visceral Leishmaniasis and infused with bone marrow mononuclear cells

Abstract

A B S T R A C TThe respiratory system has a direct contact with external environment, but this anatomical feature can be the entry route to many agents of injury. Lung disorders are not rare in clinical practice and analysis of bronchoalveolar lavage fluid (BALF) becomes an useful tool to assess possible changes in lung cell population against local injury, cellular embolism and after infusion of bone marrow mononuclear cells (BMMC) intravenously (IV). We evaluated qualitatively and quantitatively the cellular components of BALF from dogs naturally infected by Leishmania infantum chagasi and compared with infected animals and infused with BMMC. Thirteen animals were divided into negative control group (N = 6 dogs) and naturally infected (N = 7 dogs), and four dogs in this group were infused IV with BMMC homologously. The collect of BAL was performed with endotracheal tube; BMMC were obtained upon puncture of the iliac crest, separated by density gradient and adjusted a final volume containing 1 × 10 8 cells to IV infusion in each dog. The data obtained showed a significant difference in total count of cells between control and naturally infected group, showing a decrease in the pulmonary cell population in animals with leishmaniasis. The treated group showed an increase in the total of cells presents in the BALF from each dog, but the overall average there was no significant difference. In the same group there was a decrease in polymorphonuclear cells count and an increase of mononuclear cells (p≤0.05). The cellular analysis of BALF showed that pulmonary immune response is significantly depressed in canine visceral leishmaniasis and mononuclear cells population rises after BMMC infusion. K E Y W O R D SDogs; Visceral leishmaniasis; Stem cells; Cytology R E S U M OO sistema respiratório apresenta contato direto com o ambiente externo, e esta característica anatômica torna-se uma porta de entrada para diversos agentes agressores. Alterações pulmonares não são raras na rotina clínica e a análise do fluído do lavado broncoalveolar (FLBA) torna-se uma ferramenta útil para avaliar possíveis alterações na população celular pulmonar frente a situações de injúria local, embolia celular e após infusão IV de células mononucleares da medula óssea (CMMO). Objetivou-se avaliar qualitativamente e quantitativamente os componentes celulares no FLBA de cães naturalmente infectados pela Leishmania infantum chagasi e comparar com animais infectados e infundidos com CMMO. Foram utilizados treze animais, separados em grupos controle negativo (N=6 cães) e infectados naturalmente (N=7 cães), sendo que quatro cães deste grupo foram infundidos IV com CMMO homologamente. A coleta do FLBA foi realizada com sonda orotraqueal; as CMMO foram obtidas diretamente por punção da crista ilíaca, separadas por gradiente de densidade e ajustada a concentração de 1x10 8 células para infusão IV em cada cão. Os dados obtidos do FLBA revelaram uma diferença significativa no número total de células entre os grupos controle e naturalmente infectado, mostra...