Cytological Analyses by Advanced Electron Microscopy

Ichinose, Takako M.; Iwane, Atsuko H.

doi:10.1007/978-981-10-6101-1_9

Cited by 8 publications

(10 citation statements)

References 39 publications

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“…Samples for FIB-SEM were prepared using Spurr’s Low-Viscosity resin according partially modified to previously described 39 and observed. Kif3a −/− embryos at the one-somite stage harboring an NDE - Kif3a transgene were collected in ice-cold PBS and fixed immediately by incubation overnight at 4°C in fresh 0.1 M cacodylate buffer (pH 7.4) containing 2% glutaraldehyde and 4% paraformaldehyde.…”

Section: Methodsmentioning

confidence: 99%

Immotile cilia of the mouse node sense a fluid flow–induced mechanical force for left-right symmetry breaking

Katoh

Omori

Mizuno

et al. 2022

Preprint

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Immotile cilia of crown cells at the node of mouse embryos are required for sensing of a leftward fluid flow1 that gives rise to the breaking of left–right (L–R) symmetry2. The flow–sensing mechanism has long remained elusive, however, with both mechanosensing and chemosensing models having been proposed1,3–5. Here we show that immotile cilia at the mouse node respond to mechanical force. In the presence of a leftward flow, immotile cilia on the left side of the node bend toward the ventral side whereas those on the right side bend toward the dorsal side. Application of mechanical stimuli to immotile cilia along the dorsoventral axis by optical tweezers induced Ca2+ transients and degradation of Dand5 mRNA–the first known L–R asymmetric molecular events–in the targeted cells. The Pkd2 channel protein was found to be preferentially localized to the dorsal side of immotile cilia on both left and right sides of the node, and the observed induction of Ca2+ transients preferentially by mechanical stimuli directed toward the ventral side could explain the differential response of immotile cilia to the directional flow. Our results thus suggest that immotile cilia at the node sense the direction of fluid flow in a manner dependent on a flow–generated mechanical force.

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Section: Methodsmentioning

confidence: 99%

Immotile cilia of the mouse node sense a fluid flow–induced mechanical force for left-right symmetry breaking

Katoh

Omori

Mizuno

et al. 2022

Preprint

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“…Samples for FIB-SEM were prepared and observed as previously described (39). A mouse embryo at one-somite stage was collected in…”

Section: Ca 2+ Imagingmentioning

confidence: 99%

Role of Ca ²⁺ transients at the node of the mouse embryo in breaking of left-right symmetry

et al. 2020

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Immotile cilia sense extracellular signals such as fluid flow, but whether Ca2+ plays a role in flow sensing has been unclear. Here, we examined the role of ciliary Ca2+ in the flow sensing that initiates the breaking of left-right (L-R) symmetry in the mouse embryo. Intraciliary and cytoplasmic Ca2+ transients were detected in the crown cells at the node. These Ca2+ transients showed L-R asymmetry, which was lost in the absence of fluid flow or the PKD2 channel. Further characterization allowed classification of the Ca2+ transients into two types: cilium-derived, L-R-asymmetric transients (type 1) and cilium-independent transients without an L-R bias (type 2). Type 1 intraciliary transients occurred preferentially at the left posterior region of the node, where L-R symmetry breaking takes place. Suppression of intraciliary Ca2+ transients delayed L-R symmetry breaking. Our results implicate cilium-derived Ca2+ transients in crown cells in initiation of L-R symmetry breaking in the mouse embryo.

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“…Interpretasi sel hidup yang ditangkap dalam waktu lama menggunakan mikroskop elektron pemindaian canggih (SEM) berguna untuk mengidentifikasi perilaku sel sekuensial dan perubahan lokal berdasarkan lingkungannya (Golding, C., 2016) [21]. Namun, dalam beberapa tahapan organel dalam sel alga merah uniseluler primitif Cyanidioschyzon merolae (C. merolae) selama pembelahan sel akan muncul dalam berbagai posisi dan waktu.…”

Section: Studi Kasus: Kecerdasan Buatan Untuk Melakukan Segmentasi Sel Ke Dalam Beberapa Jenis Organelunclassified

Peran Big Data dan Deep Learning untuk Menyelesaikan Permasalahan Secara Komprehensif

Yudistira¹

2021

Expert J. Manaj. Sist. Inf. dan Teknol.

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Peran sain data besar (Big Data) dan pembelajaran mesin dewasa ini tidak dapat terelakkan terutama untuk menganalisis data dan memberikan kecerdasan pada komputer agar bekerja secara otonom untuk menyelesaikan suatu pekerjaan tertentu. Perkembangan teknologi sensor dan internet membuat ketersediaan data tersebut melimpah yang selanjutnya dapat dilakukan analisis data dalam jumlah yang besar. Hal tersebut mempengaruhi bagaimana cara pandang komputasi dalam berbagai macam bidang baik ilmu alam maupun sosial. Data yang terkumpul dapat berupa beragam format dengan laju pertambahan yang cepat dan dinamis. Kita perlu algoritma atau model yang mumpuni untuk memahami dan menggali pengetahuan pada set data yang besar tersebut beserta rancangan modelnya yang secara otomatis mempunyai kemampuan memprediksi atau mendeteksi. Deep Learning dengan kapasitasnya yang besar serta hubungan korelasi antar neuron yang sangat banyak diharapkan mampu menjawab tantangan tersebut didukung oleh beberapa penelitian terkini pada penerapannnya di berbagai bidang keilmuan. Dalam paper ini akan dipaparkan contoh pemanfaatan Deep Learning pada Big Data yang telah kita lakukan pada pengenalan video aksi manusia pada Youtube, Segmentasi pada sel berskala besar, citra dada x-ray dan data time-series multi variabel hubungannya dengan pandemi COVID-19.

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Cytological Analyses by Advanced Electron Microscopy

Cited by 8 publications

References 39 publications

Immotile cilia of the mouse node sense a fluid flow–induced mechanical force for left-right symmetry breaking

Immotile cilia of the mouse node sense a fluid flow–induced mechanical force for left-right symmetry breaking

Role of Ca ²⁺ transients at the node of the mouse embryo in breaking of left-right symmetry

Peran Big Data dan Deep Learning untuk Menyelesaikan Permasalahan Secara Komprehensif

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Cytological Analyses by Advanced Electron Microscopy

Cited by 8 publications

References 39 publications

Immotile cilia of the mouse node sense a fluid flow–induced mechanical force for left-right symmetry breaking

Immotile cilia of the mouse node sense a fluid flow–induced mechanical force for left-right symmetry breaking

Role of Ca 2+ transients at the node of the mouse embryo in breaking of left-right symmetry

Peran Big Data dan Deep Learning untuk Menyelesaikan Permasalahan Secara Komprehensif

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Role of Ca ²⁺ transients at the node of the mouse embryo in breaking of left-right symmetry