Cytologic, histopathologic, and clinical features of granulomatous colitis in a French Bulldog

Conrado, Francisco de Oliveira; Jones, Emery A; Graham, Erin A; Simpson, Kenneth W.; Craft, William F.; Beatty, Sarah S K

doi:10.1111/vcp.12944

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…A few case reports have also documented the use of FISH in the detection of canine E. coli infection. Conrado et al [ 45 ] reported the use of FISH specific to the detection of E. coli to confirm cytology performed on rectal scrapings. The samples were collected from a French Bulldog diagnosed with granulomatous colitis, which was otherwise unresponsive to treatment.…”

Section: The Use Of Fish For the Detection Of Intracellular Bacteria ...mentioning

confidence: 99%

Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals

Rolph,

Bolfa,

Cavanaugh

et al. 2024

Veterinary Sciences

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FISH techniques have been applied for the visualization and identification of intracellular bacteria in companion animal species. Most frequently, these techniques have focused on the identification of adhesive-invasive Escherichia coli in gastrointestinal disease, although various other organisms have been identified in inflammatory or neoplastic gastrointestinal disease. Previous studies have investigated a potential role of Helicobacter spp. in inflammatory gastrointestinal and hepatic conditions. Other studies evaluating the role of infectious organisms in hepatopathies have received some attention with mixed results. FISH techniques using both eubacterial and species-specific probes have been applied in inflammatory cardiovascular, urinary, and cutaneous diseases to screen for intracellular bacteria. This review summarizes the results of these studies.

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Section: The Use Of Fish For the Detection Of Intracellular Bacteria ...mentioning

confidence: 99%

Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals

Rolph,

Bolfa,

Cavanaugh

et al. 2024

Veterinary Sciences

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“…Granulomatous colitis (GC) is an inflammatory enteropathy common among Boxers and French bulldogs characterised by large-bowel diarrhoea, haematochezia and tenesmus. 1 – 6 The histological appearance of GC is well described, with severe mucosal ulceration and periodic acid–Schiff positive (PAS+) macrophages dispersed throughout the colonic lamina propria and submucosa. 2 , 7 – 9 Invasive and adherent Escherichia coli strains have been identified within PAS+ macrophages using fluorescent in situ hybridisation (FISH).…”

Section: Introductionmentioning

confidence: 99%

Repeat histopathology and culture of colonic biopsy specimens after treatment for Escherichia coli-associated granulomatous colitis in a cat

Waite,

Yaffy,

Kathrani

2023

Journal of Feline Medicine and Surgery Open Reports

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Case summary A 7.5-year-old neutered male Oriental Shorthair cat presented with an 8-month history of haematochezia, mucoid diarrhoea, tenesmus and vocalisation after a 4-year history of small bowel diarrhoea. Transabdominal ultrasonography confirmed diffuse colonic wall thickening and extensive ulceration and erythema after colonoscopy. Colonic histopathology confirmed periodic acid-Schiff positive macrophages, consistent with granulomatous colitis; Escherichia coli was cultured from colonic biopsy specimens. Fluorescent in situ hybridisation (FISH) identified intracellular E coli, and an 8-week oral course of marbofloxacin, a hydrolysed protein diet and a 5-day course of fenbendazole yielded a transient partial clinical remission of the colitis signs. A reported resolution in the small bowel signs was also reported. Colonoscopy was repeated 5 months later due to the recurrence of colitis signs. Histopathology was not consistent with granulomatous colitis supporting a complete remission; however, a chronic inflammatory enteropathy was confirmed with moderate lymphoplasmacytic, neutrophilic and eosinophilic colitis without a histiocytic component. E coli was again cultured from colonic biopsies with sensitivity to fluoroquinolones; FISH was positive for intracellular E coli. Clinical signs persisted despite a 2-week course of oral marbofloxacin. Relevance and novel information E coli-associated granulomatous colitis is rare in cats. Colonic biopsy specimen culture is important to guide appropriate antibiotic therapy. Repeat histopathology, culture and FISH have not been previously reported after treatment of a cat with E coli-associated granulomatous colitis. Persistent clinical signs after treatment with oral marbofloxacin alongside a confirmed complete histologic remission support the presence of a concurrent chronic inflammatory enteropathy and pathology for the cat’s ongoing colitis.

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“…4 Given that AIEC has been implicated as the primary pathogen involved in canine GC, current testing relies on a combination of bacterial culture, fluorescence in situ hybridization (FISH), or immunohistochemistry (IHC) to detect this organism in the affected tissues. 2,8,13 FISH is a time-consuming and potentially costly test and may not always be practical in a diagnostic setting. We investigated the potential use of in situ hybridization (ISH) for E. coli in cases of canine GC to further identify the causative agent.…”

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confidence: 99%

In situ hybridization to detect Escherichia coli in canine granulomatous colitis

Smith,

Miller

2023

J VET Diagn Invest

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Canine granulomatous colitis (histiocytic ulcerative colitis) is an uncommon disease, predominantly of young French Bulldogs and Boxer dogs, that manifests from a dysregulated immune response, primarily to adherent-invasive Escherichia coli (AIEC). In conjunction with histopathology and periodic acid–Schiff staining, the diagnosis of granulomatous colitis currently relies on fluorescence in situ hybridization (ISH) or immunohistochemistry to identify and localize AIEC organisms within macrophages in the mucosa and/or submucosa. We investigated the utility of ISH for E. coli using formalin-fixed, paraffin-embedded specimens collected from 29 cases of suspected granulomatous colitis. Most confirmed cases of granulomatous colitis were in French Bulldogs (12 of 20; 60%) and Boxers (3 of 20; 15%), and the mean age was 25 ± 6 mo with no sex predilection. E. coli ISH signal localized bacterial genetic material within the mucosa in 20 of 29 (69%) cases, supporting the diagnosis. ISH signal was limited to the lumen in 8 of 29 (28%) cases, which did not support the identification of these organisms as AIEC. The remaining case had no hybridization signal, and the diagnosis of granulomatous colitis was not supported. Our results revealed that ISH is a quick and specific detection method that can effectively confirm the diagnosis of canine granulomatous colitis.

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Cytologic, histopathologic, and clinical features of granulomatous colitis in a French Bulldog

Cited by 4 publications

References 13 publications

Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals

Fluorescent In Situ Hybridization for the Detection of Intracellular Bacteria in Companion Animals

Repeat histopathology and culture of colonic biopsy specimens after treatment for Escherichia coli-associated granulomatous colitis in a cat

In situ hybridization to detect Escherichia coli in canine granulomatous colitis

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