Cytokinesis block micronucleus assay in field plants for monitoring radiation-induced genotoxicity of the environment

Watanabe, Yoshito; Kubota, Yoshihisa; Fuma, Shoichi; Maruyama, Kouichi; Ichikawa, San’ei; Kubota, Masahide; Yoshida, Satoshi

doi:10.1016/j.mrgentox.2014.08.009

Cited by 3 publications

(3 citation statements)

References 29 publications

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“…The monitoring and screening of the genotoxic effects of the potentially toxic chemicals have a good indicator: High plants. The A. cepa is a high plant used commonly in evaluating the effects of the chemicals on genetic materials [21][22].…”

Section: Discussionmentioning

confidence: 99%

Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator

Türkoğlu

2021

Cumhuriyet Science Journal

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In this study, the cytotoxic and genotoxic effects of Reactive Blue 19 and Reactive Black 5 were investigated using the Allium test and comet assay. These chemicals are fabric dyes used in textile industries in various parts of Turkey. Bulbs with roots of Allium cepa L. were treated with different concentrations (25, 50 and 100 ppm) of these textile dyes for 24 h. and 48 h. The root tips were processed for cytological studies by the aseto-orcein squash procedure. Distilled water and methyl methane sulfonate (MMS, 10 ppm) were used as an negative and positive control, respectively. Exposure of Reactive Blue 19 and Reactive Black 5 significantly decreased mitotic index values. Additionally, all treatments changed the frequency of mitotic phases when compared with the control groups. These dyes increased chromosome aberrations in test material. Among these abnormalities were anaphase bridges, c-mitosis, laggards, micronuclei and stickiness. A significant increase in DNA damage was also observed at all concentrations of both Reactive Blue 19 and Reactive Black 5 examined by comet assay.

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Section: Discussionmentioning

confidence: 99%

Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator

Türkoğlu

2021

Cumhuriyet Science Journal

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“…Of particular interest are the in situ studies carried out on plants, naturally living in contaminated areas, and plant test-systems. The influence of radiation accidents [1,2], heavy metal pollution [3,4], pesticides and oil products [5][6][7], industrial enterprises [8], as well as the conditions of urban areas [9][10][11] was studied using these methods.…”

Section: Introductionmentioning

confidence: 99%

The Dynamics of Anthropogenic Polluted Areas in Kabardino-Balkaria and Chechen Republic

Reutova¹,

Dzambetova²,

Dreeva³

et al. 2020

Proceedings of the IV International Scientific and Practical Conference 'Anthropogenic Transformation of Geospace: Nature, Econ

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The dynamics of the territories exposed to significant anthropogenic pollution by heavy metals (Kabardino-Balkar Republic) and oil products (Chechen Republic) were studied. The level of soil pollution and its genotoxic effect were determined during the intensive activity of the enterprises-sources of pollution, and 10-17 years after their liquidation. The level of soil contamination with heavy metals was determined using the method of X-ray fluorescence analysis, petroleum products by infrared spectroscopy and benz(a)pyrene by spectrofluorometric analysis at low temperatures. To determine the genotoxic effect of pollution we used plants naturally growing in the study areas (Taraxacum officinale Wigg. s.l.). We applied anaphase/telophase method in root meristem cells to identify the frequency of chromosomal aberrations. The level of mutations in plants in contaminated areas increased by 4.5-6.7 times during the work of enterprises. After the liquidation of enterprises, the level of pollution decreased. Mutation rate also declined but remained higher than in the clean zones by 3.3-3.5 times. Thus, it was found that, despite the decrease in soil contamination, the genotoxic effect persists.

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“…Effective biomonitoring for the detection of radiation-induced genotoxicity of radioactive contamination in natural environment involves testing of blood lymphocyte of local residence for cytogenetic changes. [2] Blood, which can be easily accessed, represents a convenient compartment to assess radiation bioindicators. A variety of mutagenicity test procedures has been developed which enables the detection of DNA damage caused by ionizing radiation.…”

Section: Introductionmentioning

confidence: 99%

Micronucleus Assay-based Evaluation of Radiosensitivity of Lymphocytes among Inhabitants Living in High Background Radiation Area of Mamuju, West Sulawesi, Indonesia

et al. 2018

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Naturally occurring radiation can be found all around us and account for most of the radiation received by human beings each year. Indonesia has a region with high-dose natural radiation located in the suburb of West Sulawesi province with a dose rate up to 2800 nSv/h; however, its impact was not fully understood. The aim of this study was to evaluate the radiosensitivity of 12 peripheral blood lymphocytes of inhabitant from high background radiation area (HBRA) and 10 from normal background radiation area (NBRA) based on cytokinesis-block micronucleus (CBMN) assay after challenged with 1.5 Gy of gamma ray. The analysis of CBMN was done according to standard procedure as per IAEA guidelines, and frequency of binucleate (mitotic) cells with micronuclei (MN) was scored in around 2000 binucleate lymphocytes cells per culture in microscopic analysis. Mean MN frequency for HBRA was lower than that of NBRA (0.121 vs. 0.189) after irradiation, indicating an adaptive response in HBRA group that resulted in less radiosensitivity; however, there was no statistically significant different ( P > 0.05) between these two groups. The MN number was higher in women compared to men for both HBRA (0.15 vs. 0.09) and NBRA (0.216 vs. 0.147) groups. Besides, there was no statistically significant difference ( P > 0.05) in Nuclear Division Index (NDI), as measured in 500 metaphase cells with published formula, between HBRA and NBRA samples (1.24 vs. 1.21). The lower MN frequency prompts us to conclude that there is an adaptive response in the lymphocytes of inhabitants as an indicator of lower radiosensitivity to the high natural radiation exposure. Further studies using large number of samples are required to obtain more comprehensive conclusion along with the assessment of other types of radiosensitivity-related biomarkers.

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Cytokinesis block micronucleus assay in field plants for monitoring radiation-induced genotoxicity of the environment

Cited by 3 publications

References 29 publications

Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator

Genotoxic effect of two commonly used textile dyes Reactive Blue 19 and Reactive Black 5 using Allium cepa L. as an indicator

The Dynamics of Anthropogenic Polluted Areas in Kabardino-Balkaria and Chechen Republic

Micronucleus Assay-based Evaluation of Radiosensitivity of Lymphocytes among Inhabitants Living in High Background Radiation Area of Mamuju, West Sulawesi, Indonesia

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