Cytokines transduced bone marrow stromal cell lines promote immunohematopoietic reconstitution in mice after allogeneic bone marrow transplantation

Li, Ailing; Jiang, Jiyang; Zhang, Qingyin; Hao, Jie; Xie, Sai‐Sai

doi:10.1016/j.imlet.2004.11.018

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…Murine MSC were transfected with the murine IL-3 and/ or IL-2 genes, then injected into lethally irradiated C57BL/6 mice with allogeneic T cell depleted bone marrow. The cytokine-transduced stromal cells significantly increased the numbers of hematopoietic progenitors and lymphocytes derived from the co-transplanted HSC 63 .…”

Section: Systemic Cytokine Production From Genetically Engineered mentioning

confidence: 99%

Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors

Meyerrose

Olson

Pontow

et al. 2010

Advanced Drug Delivery Reviews

157

119

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Section: Systemic Cytokine Production From Genetically Engineered mentioning

confidence: 99%

Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors

Meyerrose

Olson

Pontow

et al. 2010

Advanced Drug Delivery Reviews

157

119

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“…IL-6 promotes the production of self-reactive antibodies by B lymphocytes, shows a clonogenic stimulating activity on HSCs and causes an increase in myelopoiesis 40 . Other ILs can synergistically promote the proliferation and differentiation of HSCs 41 – 43 . In particular, the activation of hematopoietic cells regulated by IL-3 and IL-5 is related to the activation of STAT3 and JAK2 44 , 45 .…”

Section: Discussionmentioning

confidence: 99%

Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway

et al. 2018

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Sarcodon imbricatus, a rare medicinal and edible fungus, has various pharmacological bioactivities. We investigated the effects of S. imbricatus polysaccharides (SIPS) on hematopoietic function and identified the underlying mechanisms using in vitro experiments with CHRF, K562, and bone marrow mononuclear cells (BMMNCs) and in vivo experiments with a mouse model of cyclophosphamide-induced hematopoietic dysfunction. We found that SIPS induced proliferation and differentiation of CHRF and K562 cells and upregulated the expression of hematopoietic-related proteins, including p90 ribosomal S6 kinases (RSK1p90), c-Myc, and ETS transcription factor, in the two cell lines. After 28 days of treatment, SIPS enhanced the bodyweight and thymus indices of the mice, alleviated enlargement of the spleen and liver, and contributed to the recovery of peripheral blood to normal levels. More importantly, the percentages of B lymphocytes and hematopoietic stem cells or hematopoietic progenitor cells were significantly elevated in bone marrow. Based on an antibody chip analysis and enzyme-linked immunosorbent assay, SIPS were found to successfully regulate 12 cytokines to healthy levels in serum and spleen. The cytokines included the following: interleukins 1Ra, 2, 3, 4, 5, and 6, tumor necrosis factor α, interferon−γ, granulocyte colony-stimulating factor (G-CSF) and macrophage colony-stimulating factor (M-CSF), C-C motif chemokine1, and monocyte chemoattractant protein−1. Moreover, SIPS upregulated the phosphorylation levels of janus kinase 2 (JAK2) and the signal transducer and activator of transcription 3 (STAT3) in the spleen, and similar results were validated in CHRF cells, K562 cells, and BMMNCs. The data indicate that SIPS activated the JAK2/STAT3 pathway, possibly by interactions among multiple cytokines, particularly G-CSF. We found that SIPS was remarkably beneficial to the bone marrow hematopoietic system, and we anticipate that it could improve myelosuppression induced by long-term radiotherapy or chemotherapy.

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Antileukemic Effect of Interleukin-7-Transduced Bone Marrow Stromal Cells in Mice Following Allogeneic T-Cell-Depleted Bone Marrow Transplantation

Feng

et al. 2005

Transplantation Proceedings

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Cytokines transduced bone marrow stromal cell lines promote immunohematopoietic reconstitution in mice after allogeneic bone marrow transplantation

Cited by 4 publications

References 34 publications

Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors

Mesenchymal stem cells for the sustained in vivo delivery of bioactive factors

Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway

Antileukemic Effect of Interleukin-7-Transduced Bone Marrow Stromal Cells in Mice Following Allogeneic T-Cell-Depleted Bone Marrow Transplantation

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