Cytokines stimulate matrix metalloproteinase production by human pulp cells during long-term culture

O'Boskey, F. James; Panagakos, Fotinos

doi:10.1016/s0099-2399(98)80203-3

Cited by 46 publications

(35 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…47 Bacterial products irritate PMNs to release MMP-8 48,49 and proinflammatory cytokines upregulate MMP-1 and MMP-2 in the pulp tissue. 45,50,51 Bacteria and their products tend to act in pulpal inflammation by upregulating cytokine production, via the cytokine pathway, increase MMP expression and directly irritate cells to produce MMPs. PMN cells are involved in pulp abscess formation and thus activated MMP-8 participate in the tissue destruction of pulp necrosis and abscess.…”

Section: Role Of Mmps In Pulpal Inflammationmentioning

confidence: 99%

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Jain¹,

Bahuguna²

2015

Journal of Oral Biology and Craniofacial Research

View full text Add to dashboard Cite

Section: Role Of Mmps In Pulpal Inflammationmentioning

confidence: 99%

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Jain¹,

Bahuguna²

2015

Journal of Oral Biology and Craniofacial Research

View full text Add to dashboard Cite

“…[23][24][25] MMP-1 mRNA expression was detected in all of cells studied, and secretion of MMP-1 was increased from all kinds of cell studied in dose dependent manner 24 hours after stimulation with TNF-α .…”

Section: Discussionmentioning

confidence: 91%

The effect of tumor necrosis factor (TNF)-α to induce matrix metalloproteinase (MMPs) from the human dental pulp, gingival, and periodontal ligament cells

Rhim

Park

Kim

et al. 2011

J Korean Acad Conserv Dent

View full text Add to dashboard Cite

Objectives: In the present study, three kinds of tissues cells (pulp, gingiva, and periodontal ligament) were investigated if those cells express MMP and TIMP when they were stimulated with neuropeptides (substance P, CGRP) or proinflammatory cytokine, TNF-α . Materials and Methods:The cells cultured from human dental pulp (PF), gingiva (GF) and periodontal ligament were (PDLF) stimulated with Mock, SP, TNF-α , and CGRP for 24 hrs and 48 hrs. for an RNase protection assay and Enzyme Linked Immunosorbent Assay. Cells (PF, GF and PDLF) seeded in 100 mm culture dish were stimulated with SP (10 -5, 10 -8 M) or only with medium (Mock stimulation) for 4hrs and for 24 hrs for RNase Protection Assay, and they were stimulated with CGRP (10 -5 M) and TNF-α(2 ng/mL) for 24 hrs and with various concentraion of TNF-α(2, 10, and 100 ng/mL) for Rnase Protection Assay with a human MMP-1 probe set including MMP 1, 2, 8, 7, 8, 9, 12, and TIMP 2, 3. In addition, cells (PF, GF and PDLF) were stimulated with Mock and various concentraion of TNF-α(2, 10, and 100 ng/mL) for 24 hrs and with TNF-α(10 ng/mL) for 48 hrs, and the supernatents from the cells were collected for Enzyme Linked Immunosorbent Assay (ELISA) for MMP-1 and MMP-13. Results: The expression of MMPs in PF, GF, PDLF after stimulation with SP and CGRP were not changed compared with Mock stimulation for 4 hrs and 24 hrs. The expression of MMP-1, -12, -13 24 hrs after stimulation with TNF-αwere upregulated, however the expression of TIMP-3 in PF, GF, PDLF after stimulation with TNF-αwere downregulated. TNF-α(2 ng/mL, 10 ng/mL, 100 ng/mL) increased MMP-1 and MMP-12 expression in PF dose dependently for 24 hrs. Conclusions: TNF-αin the area of inflammation may play an important role in regulating the remodeling of dentin, cementum, and alveolar bone.

show abstract

“…Morand et al (15) reported increased elastinolytic and collagenolytic activity in diseased human dentalpulp with suppurativepulpitisand necrosis. O'Boskey et al (16) reported that cytokines stimulated the production of MMPs in HDP in long-term culture. However, the end result of the inflammatory process is necrosis of the dental pulp, and, therefore, the process of acute dental pulpitis is irreversible.…”

Section: Discussionmentioning

confidence: 99%

Effects of IL-6 and IL-1β on MMP Activities in Fibroblastic Cells Derived from Human Dental Pulp and Gingiva

Aiura¹,

Matsushima

2003

Int J Oral-Med Sci

View full text Add to dashboard Cite

In order to find functional characteristics of human dental pulp in the irreversible progress of pulpitis, we examinedthe effects of IL-1 (3 and IL-6 on matrixmetalloprotease (MMP)activity in human-dental-pulp derivedfibroblastic cells (HDP) compared with human gingival-derived fibroblasts (HGF). The activities of MMPsactivated by EL-1 (3 or EL-6 in HDP and HGF were investigated with zymography. MMP-2 activity in conditioned media of both cells cultured with EL-1P or IL-6 were activated,but MMP-9 activity was not activatedwithout plasminogen.When plasminogen was added to the conditioned medium, plasmin and MMP-9 were activated. Plasmin and MMP-9 of HDP-treated IL-6 were more activated than HGF. Next, we examined the effect of these cytokines on the plasminogenactivator(PA)/plasmin system.The PAactivityin the conditioned medium showed no difference when both cells were incubated with IL-1(3. In contrast, when both cells were incubated with IL-6, the PA activity of HDP was higher than that of HGF. By reverse-transcription polymerase chain reaction (RT-PCR), tPAmRNA levels in both cells were enhanced by DL-lp and IL-6, Key words:but uPA was not effected. IL-6-stimulated tPA mRNA level of HDP was higher dental pulp fibroblast, gingival fibroblast, than that of HGF. These findings suggest that IL-6 may play a major role in plasminogen activator, MMP, extracellularmatrix (ECM) degradation through stimulation of the PA/plasmin inflammatorycytokine1|3. system in HDP of pulpitis.

show abstract

Cytokines stimulate matrix metalloproteinase production by human pulp cells during long-term culture

Cited by 46 publications

References 18 publications

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

Role of matrix metalloproteinases in dental caries, pulp and periapical inflammation: An overview

The effect of tumor necrosis factor (TNF)-α to induce matrix metalloproteinase (MMPs) from the human dental pulp, gingival, and periodontal ligament cells

Effects of IL-6 and IL-1β on MMP Activities in Fibroblastic Cells Derived from Human Dental Pulp and Gingiva

Contact Info

Product

Resources

About