Cytokines profile in knee cartilage of rats during monoiodoacetate-induced osteoarthritis and administration of probiotic

Korotkyi, Oleksandr; Vovk, A.; Halenova, Tetiana; Vovk, Tetiana; Dvorshchenko, K.; Falalyeyeva, Tetyana; Li, Ostapchenko

doi:10.7124/bc.000a1e

Cited by 16 publications

(11 citation statements)

References 66 publications

(48 reference statements)

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“…Intra-articular MIA injection can increase ROS levels that cause chondrocyte death and trigger cartilage deformation, and elevate articular IL-1β, IL-6, COX2 46 , TNF-α, and NF-κB levels 23 , while reducing TGF-β levels in rats 8 . We found that undenatured collagen supplementation decreased most inflammatory cytokine activity, cartilage degradation markers (including MMP3 and COMP), and joint swelling used for joint inflammation monitoring in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

“…IL-1 plays a central role in immune disorders by activating intracellular T-cell interactions. IL-1β and TNFα are essential triggers of OA development, whereas IL-4, transforming growth factor β (TGF-β), and interferon-gamma (IFNγ) are categorized as anti-inflammatory cytokines in OA 8 . The increase in inflammatory cytokines and reactive oxygen species (ROS) with aging also stimulate matrix metalloproteinases (MMPs) that drive cartilage degradation in OA 9,10 .…”

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confidence: 99%

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The effect of oral administration of undenatured type II collagen on monosodium iodoacetate-induced osteoarthritis in young and old rats

Şahin

Orhan

Erten

et al. 2023

Sci Rep

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We investigated whether different doses of undenatured type II collagen (undenatured collagen, UC-II) help improve monosodium iodoacetate (MIA)-induced (osteoarthritis) OA in young and old rats. A total of 70 rats were divided into five groups: (1) control; (2) MIA (a single intra-articular injection of MIA); (3)–(5) MIA+ Undenatured Collagen with various oral doses (0.66, 1.33, and 2 mg/kg). The results showed that all doses of undenatured collagen in both age groups reduced knee diameter, while the two higher doses (1.33 mg/kg and 2 mg/kg) reduced the Mankin score and increased most gait measurements as early as day 14 compared to the MIA rats. However, the 2 mg/kg dose showed the best efficacy in improving Mankin score and gait measurements by 28 days post-OA induction. In young but not old rats, all doses of undenatured collagen reduced the Kellgren-Lawrence score compared to the MIA group. Undenatured collagen reduced the levels of most inflammatory and cartilage breakdown markers in serum and knee joint cartilage in both age groups. In conclusion, this data suggests that while all doses of undenatured collagen supplementation may ameliorate MIA-induced OA symptoms, the higher doses showed faster improvement in gait measurements and were more efficacious for overall joint health in rats.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The effect of oral administration of undenatured type II collagen on monosodium iodoacetate-induced osteoarthritis in young and old rats

Şahin

Orhan

Erten

et al. 2023

Sci Rep

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“…Probiotic (PB) groups were administered an oral solution of "Multiprobiotic Simbiter®" (Company "О.D.Prolisok", Ukraine) -a biomass of living cells from the symbiosis of 17 strains of microorganisms physiological for mammalian intestines belonging to 11 species. Most strains were taken from All- The choice of this probiotic is due to its effective multicomponent live bacteria composition and previously shown by us anti-inflammatory and antioxidant properties for this pathology [10,11].…”

Section: Materials and Methods Animals And Treatmentsmentioning

confidence: 99%

The Gut Microbiota of Rats under Experimental Osteoarthritis and Administration of Chondroitin Sulfate and Probiotic

Korotkyi¹,

Luhovska²,

Serhiychuk³

et al. 2020

Mikrobiol. Z.

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Osteoarthritis is a most widespread chronic degenerative joint disease that causes pain, cartilage deformation, and joint inflammation. Adverse alterations of intestinal microbiota like dysbiosis may lead to metabolic syndrome and inflammation, two important components of osteoarthritis progression. Aim. In this study we investigated the effect of chondroitin sulfate and probiotics on the gut microbiome in monoiodoacetate-induced osteoarthritis model in rats. Methods. The species and quantitative composition of feces were determined using diagnostic media with selective properties. Further identification of isolated microorganisms was carried out according to morphological, tinctorial, physiological and metabolic parameters. The results are presented in the form of lg CFU/g. Results. Induction of osteoarthritis caused significant increasing the number of opportunistic enterobacteria and lactose-negative Escherichia coli against the decreasing of lacto- and bifidobacteria that may indicate a dysbiotic condition. Coadministration of chondroitin sulfate and probiotic bacteria has led to improvement the quantitative composition of the gut microbiota in experimental animals, the numerous of Bifidobacterium, Lactobacillus were increasing against decreasing the quantitative composition of opportunistic microorganisms. Conclusions. Monoiodoacetate-induced osteoarthritis caused dysbiosis of gut in rat. We observed beneficial effect of combined administration of chondroitin sulfate and probiotics on gut microbiota composition in rats with experimental osteoarthritis. Thus, adding of supplements like probiotics to standard treatment of osteoarthritis may have potentials to prevent and treat this disease.

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“…However, the pathophysiology of OA remains unclear. OA was recognized as systemic and local low inflammatory states [3]. Moreover, the metabolic changes such as intestinal microbiota changes also are associated with the pathogenesis of OA [4].…”

Section: Introductionmentioning

confidence: 99%

Serum periostin level is not sufficient to serve as a clinically applicable biomarker of osteoarthritis

Tan

Yang

Xing

et al. 2022

BMC Musculoskelet Disord

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Background Emerging knowledge has highlighted the role of periostin (POSTN) in osteoarthritis (OA) process; however, whether POSTN is suitable as a biomarker of OA remains unclear. This study aimed to investigate the potential value of POSTN as a biomarker of OA. Methods Ten 6-month-old female Sprague–Dawley (SD) rats were used in this study. Five rats underwent ovariectomy (OVX) operation and the others were carried out sham operation. Thirty-two patients with OA and eighteen patients who had meniscus injuries or ligament injuries but with intact articular cartilages were recruited in this study from January to July 2019 at the Peking University International Hospital. We first detected the expression of POSTN in the cartilage of OVX induced OA rats and different compartments of the knee joint in patients with OA using immunohistochemistry. Besides, serum POSTN levels in patients with or without OA were examined using enzyme-linked immunosorbent assay (ELISA). The associations among serum POSTN levels, clinical symptoms, and radiological severity were assessed according to the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scores and, Kellgren-Lawrence (KL) grading, respectively. Finally, multivariable cumulative link models were established to evaluate the validity of serum POSTN level as a predictor of knee OA. Results The significantly higher POSTN expression was found in OVX-OA rats than Sham rats, while, the expression of POSTN was significantly higher in the torn cartilage of patients with OA. However, the serum POSTN level did not differ significantly between patients with and without OA. Additionally, we found no remarkable associations between serum POSTN level and WOMAC scores and KL grading. Subsequent analysis revealed that serum POSTN was not a significant predictor of OA. Conclusion Thus, although POSTN may be involved OA process and local POSTN is valuable in disease diagnosis and distinguishing of the severity of disease, its serum level is not sufficient to serve as a candidate biomarker of OA given the current analysis technology.

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Cytokines profile in knee cartilage of rats during monoiodoacetate-induced osteoarthritis and administration of probiotic

Cited by 16 publications

References 66 publications

The effect of oral administration of undenatured type II collagen on monosodium iodoacetate-induced osteoarthritis in young and old rats

The effect of oral administration of undenatured type II collagen on monosodium iodoacetate-induced osteoarthritis in young and old rats

The Gut Microbiota of Rats under Experimental Osteoarthritis and Administration of Chondroitin Sulfate and Probiotic

Serum periostin level is not sufficient to serve as a clinically applicable biomarker of osteoarthritis

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