Cytokines, matrix metalloproteases, angiogenic and growth factors in tears of normal subjects and vernal keratoconjunctivitis patients

Leonardi, Andrea; Sathe, Sonal; Bortolotti, Massimo; Beaton, Ann; Sack, Robert A.

doi:10.1111/j.1398-9995.2008.01858.x

Cited by 94 publications

(81 citation statements)

References 34 publications

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“…The detection of various factors such as lipids [31], growth factors, proteases and cytokines [32] and small molecules such as homocysteine [33] and antioxidants [34] apart from various proteins is supportive of the fact that tear can be a potential specimen for diagnostics and prognostics. Differentially expressed proteins in tear characteristic of the clinical condition especially in corneal disorders are getting reported [23,[35][36][37][38].…”

Section: Discussionmentioning

confidence: 99%

Two dimensional electrophoretic analysis of human tears: Collection method in dry eye syndrome

Saijyothi

Angayarkanni

Syama³

et al. 2010

Electrophoresis

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Tear proteomics, by 2-DE, can give a fingerprint of the protein profile, which is well suited in clinical proteomics for biomarker identification and in diagnostics. The mode of tear collection can influence the representation of the proteins in the tear and therefore it is important to use the appropriate method. In this study, capillary and Schirmer mode of tear collection was done in the healthy controls and the Schirmer method was validated in dry eye syndrome conditions. 2-D PAGE of normal and dry eye tear was performed using pH 3-10 linear IPG strips followed by 13% SDS-PAGE. The spot intensity was analyzed by the PD quest software. The two methods were compared using Bland-Altman statistical tool. The 2-D map of capillary and Schirmer tear showed 147 ± 8 spots and 145 ± 7 spots respectively. Both the collection methods were in agreement with each other and were comparable. Dry eye tear protein showed differential expression of proteins as observed in 25-35 kDa region. One of the significantly reduced protein was identified as proline-rich 4 protein. Schirmer method of tear collection is reliable in patients with dry eye, which can display the differential protein expression and help in biomarker identification.

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Section: Discussionmentioning

confidence: 99%

Two dimensional electrophoretic analysis of human tears: Collection method in dry eye syndrome

Saijyothi

Angayarkanni

Syama³

et al. 2010

Electrophoresis

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“…The appearance of a cytokine in the tears of the patients suffering from the primary form of allergic keratoconjunctivitis has already been investigated [9,10,11,12,13,20,37,38,39,40,41,43]. In most of these studies the cytokines, such as IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-13, IFN-γ and TNF-α, have been detected by means of a single, patient-related determination in the tears [3,9,10,11,12,13,20,37,38,39,40,41,42,43]. No data concerning the concentration changes of cytokines in tears during the particular types of the primary KCR to the conjunctival provocation tests with allergens, thus response-related data, are available in the literature.…”

Section: Discussionmentioning

confidence: 99%

“…No data concerning the concentration changes of cytokines in tears during the particular types of the primary KCR to the conjunctival provocation tests with allergens, thus response-related data, are available in the literature. Moreover, little data is available concerning the possible occurrence of particular cytokines in the tears of healthy subjects, which could be exploited as reference parameters [20,22,23]. …”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, there is a dearth of data related to the course of the cytokine changes in tears during the individual KCR types of the primary KC form [1,2,3,9,10,11,12,13,15,17,19,20,37,38,39,40,41,42,43]. Moreover, no data are available to illustrate the concentration changes of the cytokines in tears accompanying the particular SKCR types [14].…”

Section: Introductionmentioning

confidence: 99%

“…The cytokines in the tears of the KC patients have already been studied from various points of view [1,5,6,14,15,16,17,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36]. Nevertheless, there is a dearth of data related to the course of the cytokine changes in tears during the individual KCR types of the primary KC form [1,2,3,9,10,11,12,13,15,17,19,20,37,38,39,40,41,42,43].…”

Section: Introductionmentioning

confidence: 99%

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Cytokines in Tears during the Secondary Keratoconjunctival Responses Induced by Allergic Reaction in the Nasal Mucosa

Pelikán¹

2014

Ophthalmic Res

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Background: Allergic keratoconjunctivitis (KC) can occur in a primary form due to an allergic reaction taking place in the conjunctivae or in a secondary form induced by nasal allergy. Objectives: To search for the cytokine changes in tears accompanying the secondary keratoconjunctival response types (SKCR), caused by the nasal allergy. Methods: In 43 KC patients developing 15 immediate (SIKCR), 16 late (SLKCR) and 12 delayed (SDYKCR) responses to nasal provocation tests with allergens (NPT), the NPTs were repeated with subsequent recording of cytokine concentrations in tears up to 72 h. Results: The SIKCRs (p < 0.001), occurring 10-120 min after the NPT, were accompanied by significant changes (p < 0.05) of interleukin (IL)-4, IL-6, IL-10, IL-12p70 and granulocyte-macrophage colony-stimulating factor (GM-CSF). The SLKCRs (p < 0.01), appearing 5-12 h after the NPT, were associated with significant changes (p < 0.05) of IL-3, IL-4, IL-5, IL-8, IL-10, tumor necrosis factor (TNF)-α, GM-CSF and granulocyte colony-stimulating factor. The SDYKCRs (p < 0.01), occurring 24-48 h after the NPT, were accompanied by significant changes (p < 0.05) of IL-2, IL-8, IL-10, interferon-γ, transforming growth factor-β and TNF-α. Conclusions: The particular SKCR types, induced by an allergic reaction in the nasal mucosa, were accompanied by different cytokine profiles in the tears, suggesting involvement of different hypersensitivity mechanisms. These results also stress the diagnostic usefulness of NPTs combined with monitoring of ocular features in KC patients who did not respond satisfactorily to the topical ophthalmological treatment.

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Antibody Microarrays in Proteome Profiling

Alhamdani

Hoheisel

2011

Molecular Analysis and Genome Discovery

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Cytokines, matrix metalloproteases, angiogenic and growth factors in tears of normal subjects and vernal keratoconjunctivitis patients

Cited by 94 publications

References 34 publications

Two dimensional electrophoretic analysis of human tears: Collection method in dry eye syndrome

Two dimensional electrophoretic analysis of human tears: Collection method in dry eye syndrome

Cytokines in Tears during the Secondary Keratoconjunctival Responses Induced by Allergic Reaction in the Nasal Mucosa

Antibody Microarrays in Proteome Profiling

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