Cytokines in culture media of preimplantation embryos during in vitro fertilization: Impact on embryo quality

Lee, Inha; Ahn, So Hyun; Kim, Hye In; Baek, Hye Won; Park, Yun Jeong; Kim, Heeyon; Aljassim, Aminah Ibrahim; Shin, Whan; Ryu, C.S.; Yoon, Jong Il; Lee, Jae Hoon; Yun, Bo Hyon; Seo, Seok Kyo; Park, Joo Hyun; Choi, Young Sik; Cho, SiHyun; Lee, Byung Seok

doi:10.1016/j.cyto.2021.155714

Cited by 6 publications

(4 citation statements)

References 34 publications

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“…FF is rich in cytokines, which was observed in our previous research [4,7,8]. The interpretation of FF cytokine concentration and its influence on ovarian follicles, the natural course of ovulation and corpus luteum formation, and eventually embryo implantation is difficult due to many confounding factors.…”

Section: Discussionmentioning

confidence: 82%

Assessment of RANTES, MIP4A, MMP7, MMP9, MMP14, TIMP 1, TIMP 2 and TIMP 3 concentration in the follicular fluid of patients undergoing in vitro fertilization/embryo transfer procedure

Adamczak¹,

Ukleja-Sokołowska²,

Lis³

et al. 2023

pdia

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Introduction: Matrix metalloproteinases (MMP) degrade extracellular matrix. Some studies show that MMP9 concentration in follicular fluid (FF) may play a role in oocyte maturation and in vitro fertilization (IVF) success. The immunology of follicular fluid is still not fully understood. Aim: Assessment of RANTES, MIP4A, MMP7, MMP9, MMP14, TIMP 1, TIMP 2 and TIMP 3 concentration in the follicular fluid of patients undergoing in vitro fertilization/embryo transfer procedure. Material and methods: This case-control study included 20 randomly selected patients with a positive pregnancy (PPG) test and 20 patients with a negative pregnancy (NPG) test after IVF/ET. In FF obtained during oocyte retrieval, the concentrations of MIP4A, MMP7, MMP14, TIMP 1, TIMP 2, TIMP 3, RANTES, IL-12p40, and IL-17A were measured. Their effect on the characteristics of follicles, embryos, and the efficiency of IVF and ET were analysed. Results: There was no statistically significant relationship between a positive pregnancy test and the results of the immunoassay performed. The number of COC-1 correlates significantly and positively with RANTES (r = 0.34; p = 0.038) and IP-10 (r = 0.329; p = 0.038). MII correlates significantly and positively with RANTES (r = 0.341, p = 0.031). The number of top-quality embryos correlates significantly and positively with IL-17A (r = 0.451, p = 0.004) and TIMP 1 (r = 0.44, p = 0.005). Conclusions: The concentration of IL-17A and TIMP 1 may predict IVF/ET success. Further studies are required on the influence of the follicular fluid immunological environment on oocyte maturation and quality and, subsequently, embryo development.

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Section: Discussionmentioning

confidence: 82%

Assessment of RANTES, MIP4A, MMP7, MMP9, MMP14, TIMP 1, TIMP 2 and TIMP 3 concentration in the follicular fluid of patients undergoing in vitro fertilization/embryo transfer procedure

Adamczak¹,

Ukleja-Sokołowska²,

Lis³

et al. 2023

pdia

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“…As performed in previous studies, the embryos were assessed as top-quality or non-top-quality. 6 Depending on the patient’s individual decision and the assessed chances of succeeding, one or two embryos were chosen for transfer to the uterus on the fifth day after fertilization. During embryo transfer (ET), the endometrium needed to be at least 7 mm thick.…”

Section: Methodsmentioning

confidence: 99%

Concentrations of matrix metallopeptidase 9, interleukin 4, and interleukin 8 in follicular fluid, and the results of in vitro fertilization

et al. 2022

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Objective Cytokines and enzymes in follicular fluid (FF) may have a crucial role in fertility. This study aimed to analyse the results of in vitro fertilization/embryo transfer (IVF/ET) and compare them with matrix metalloproteinase 9 (MMP-9) and cytokine concentrations in FF. Methods This case–control study included 20 randomly selected patients with a positive pregnancy (PPG) test and 20 with a negative pregnancy (NPG) test after IVF/ET. In FF obtained during oocyte retrieval, the concentrations of soluble forms of MMP-9, interleukin 1 (IL-1) alpha, IL-1 beta, interferon gamma, IL-2, IL-4, IL-6, IL-8, and IL-10 were measured. Their effect on the characteristics of follicles, embryos, and the efficiency of IVF and ET were analysed. Results High-sensitivity IL-4 concentrations were significantly higher in patients with a PPG test than in those with an NPG test (3.15 ± 3.23 vs 1.91 ± 0.35 pg/mL). The number of top-quality embryos achieved was significantly higher in patients with a PPG test than in those with an NPG test (2.6 ± 1.39 vs 1.75 ± 1.21), and they were negatively correlated with IL-8 concentrations in FF. Conclusion Further research on the role of IL-4 and IL-8 in FF is required to establish any clinical benefit of determining their concentrations in FF of infertile women.

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“…One of the parameters influencing the success of the IVF procedure is the state of the immune system, which is actively involved in the regulation of ovarian functions, controlling steroidogenesis, folliculogenesis, ovulation, formation and atresia of the corpus luteum. [11,12] So, some cytokines support the embryonic development includes promotion of implantation and protection of blastomeres from cell stress and apoptosis. The correlation between embryo quality and the concentration of specific cytokines is considered to be an important predictor of successful treatment.…”

Section: г і н е к о л о г і яmentioning

confidence: 99%

The role of clinical and immunological factors in the outcomes of in vitro fertilization procedure in women

Safarova

2023

RHW

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The objective: to determine the features of clinical and immunological factors in women undergoing in vitro fertilization (IVF) procedure and to determine a relationship between these parameters and the procedure outcomes.Materials and methods. In the period from 2020 to 2022, 131 patients were examined at the Reproductive Department of the Caspian International Hospital. Of these, 103 (78.6%) are aged 19-35 years, 28 (21.4%) are aged 36-50. Patients were grouped and analyzed based on: IVF success (presence or absence of fertilization), pregnancy (yes, no), and infertility factors. Markers of peripheral blood mononuclear cells (CD16/56 and HLA-DR) were determined in 50 women. The cytokines – interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interferon-ɣ (IFN-ɣ) were studied in blood serum of 84 patients. All samples were taken at the day of oocyte pick-up. Descriptive statistical methods (mean, standard deviation, median, frequency, percentage, and minimum and maximum), Student-t test and Mann–Whitney U test (for comparisons between two groups) were used while evaluating the study data. Statistical significance was accepted as p<0.05.Results. Female infertility occurred in 49 (37.4%) patients, 82 (62.6%) patients were fertile. There was no significant difference in CD16/56 and HLA-DR levels depending on pregnancy status, IVF outcomes and infertility factors (p>0.05). The levels of IL-1β and IFN-ɣ differed in the blood serum of patients with and without pregnancy (p=0.041 and p=0.037, respectively). Analysis of groups based on infertility factors showed that there were not significant differences (p>0.05) in the levels of CD16/56, HLA-DR, IL-1β, TNF-α and IFN-ɣ between groups. Conclusions. Our study showed that there is an association between serum levels of IL‒1β and IFN-y and successful pregnancy in the IVF procedure. We did not reveal the other relationships between clinical and immunological parameters in women with IVF procedure.

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Cytokines in culture media of preimplantation embryos during in vitro fertilization: Impact on embryo quality

Cited by 6 publications

References 34 publications

Assessment of RANTES, MIP4A, MMP7, MMP9, MMP14, TIMP 1, TIMP 2 and TIMP 3 concentration in the follicular fluid of patients undergoing in vitro fertilization/embryo transfer procedure

Assessment of RANTES, MIP4A, MMP7, MMP9, MMP14, TIMP 1, TIMP 2 and TIMP 3 concentration in the follicular fluid of patients undergoing in vitro fertilization/embryo transfer procedure

Concentrations of matrix metallopeptidase 9, interleukin 4, and interleukin 8 in follicular fluid, and the results of in vitro fertilization

The role of clinical and immunological factors in the outcomes of in vitro fertilization procedure in women

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