Cytokines Determining the Success or Failure of Pregnancy

Clark, David A.; Lea, Richard G.; Podor, Thomas J.; Daya, Salim; Banwatt, Daljeet; Harley, Calvin B.

doi:10.1111/j.1749-6632.1991.tb37944.x

Cited by 73 publications

(42 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TGF-(3, TGF-(2 in particular, also appears to have an immunoregulatory role in mice, which is important for the maintenance of pregnancy. It has been shown that neutralizing antibodies against TGF-,B2 appear to increase the spontaneous rate of abortion (Clark et al, 1991). Furthermore, a TGF-,B2-like immunosuppressive factor in murine amniotic fluid, which appears to be complexed with a-fetoprotein, has been identified (Altman et al, 1990).…”

Section: Identification and Characterization Of Tgf-/3mentioning

confidence: 99%

“…With respect to crosslinking effects, we have previously demonstrated that the apparently higher capacity of the primary trophoblast type III/betaglycan receptor for TGF-/1 than TGF-/2 was not due to an inherent difference in the crosslinking efficiency to 1251I-TGF-/1 versus 1251I-TGF-/32 (i.e., the type III/betaglycan receptor on control placental mesenchymal cell cultures was labeled to the same extent by 1251I-TGF-/1 and 1251I-TGF-/2 in affinity labeling saturation studies [Mitchell et al, 1992] (Mitchell et al, 1992) and some other cells types (Cheifetz et al, 1990 (Attisano et al, 1992 (MacKay and Danielpour, 1991). In addition, of the four TGF-3 binding components purified recently from porcine uterus (Ichijo et al, 1991) (Mitchell, unpublished observations The activities of TGF-f in extracellular matrix remodeling, growth inhibition, and immunosuppression have led various investigators to examine the roles of TGFf in trophoblast invasiveness (Lala and Graham, 1990;Tamada et al, 1990;Lala, 1991, 1992a,b), trophoblast differentiation (Morrish et al, 1991;, and in the maintenance of pregnancy (Altman et al, 1990;Clark et al, 1990Clark et al, , 1991Lea et al, 1992). A greater understanding of the control of trophoblast invasiveness is important for the treatment of certain conditions of pregnancy, such as the overinvasiveness of choriocarcinoma or the underinvasiveness of preeclampsia, as well as for cancer therapy in general.…”

Section: Identification and Characterization Of Tgf-/3mentioning

confidence: 99%

See 1 more Smart Citation

Characterization of transforming growth factor-beta (TGF-beta) receptors on BeWo choriocarcinoma cells including the identification of a novel 38-kDa TGF-beta binding glycoprotein.

Mitchell

Lee

O’Connor-McCourt

1992

MBoC

View full text Add to dashboard Cite

Transforming growth factor-: (TGF-f) is a potential mediator of placental trophoblast functions, including differentiation, hormone production, endometrial invasion, and immunosuppression. Equilibrium binding and affinity-labeling assays were used to investigate the binding characteristics of TGF-41 and TGF-f2 on an established human choriocarcinoma trophoblastic cell line (BeWo). The equilibrium binding experiments indicated that the BeWo cells exhibited similar average affinities and total number of binding sites for TGFf1 and TGF-,32. The Kd values obtained from Scatchard analyses were -65 pM for 125I1 TGF-31 and -40 pM for 1251-TGF-fl2, with 70 000 and 85 000 sites per cell, respectively. Competitive equilibrium binding experiments indicated that TGF-f1 and TGF-32 were equipotent (apparent half maximal inhibition [IC50] -70 pM) and that all binding sites were capable of recognizing both isoforms. Affinity-labeling studies with 1251-TGF-l1 and 1251-TGF-32 and the chemical cross-linking agent bis(sulfosuccinimidyl)suberate (BS3) revealed a predominant type III/betaglycan receptor, a low level of apparently heterogeneous type I and II receptors and an additional novel 38-kDa TGF-f binding glycoprotein that was present both under reducing and nonreducing conditions on sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Affinity-labeling saturation and competition studies indicated that the type III/betaglycan component appears to have a 7-fold higher capacity for TGF-31 than for -f2 yet exhibits a 5-to 10-fold higher affinity for TGF-,B2 than for -41. The 38-kDa TGF-1 binding component, an N-linked glycoprotein, exhibits a higher affinity for TGF-,B2 than for -f1 that is strikingly similar to that of the type III/ betaglycan receptor. This 38-kDa binding protein appears to be upregulated after methotrexate-induced differentiation of the BeWo cells.

show abstract

Section: Identification and Characterization Of Tgf-/3mentioning

confidence: 99%

Section: Identification and Characterization Of Tgf-/3mentioning

confidence: 99%

Characterization of transforming growth factor-beta (TGF-beta) receptors on BeWo choriocarcinoma cells including the identification of a novel 38-kDa TGF-beta binding glycoprotein.

Mitchell

Lee

O’Connor-McCourt

1992

MBoC

View full text Add to dashboard Cite

show abstract

“…Macrophages in human pregnancy decidua may also express CD4, unlike macrophages in the endometrium, and it is therefore possible that some of the CD4 positive cells associ¬ ated with endometrial cups in mares are actually macrophages (Clark and Daya, 1988;Clark, 1993 (Lea et al, 1992 (Clark et al, 1990(Clark et al, , 1991a(Clark et al, , b, 1993 (Raghupathy et al, 1981;Clark et al, 1987;Chaouat et al, 1988). In this regard, it is interesting that low concentrations of TGF-ß can promote IgG as well as IgA responses (Zettel et al, 1991).…”

Section: Resultsmentioning

confidence: 99%

“…A local intrauterine immunoprotective response has been reported in mice in which résorption (abortion) is associated with a deficiency of local decidual suppressor activity predomi¬ nantly mediated by a molecule closely related to transforming growth factor subtype ß2 (TGF-ß2) (Clark et al, 1986(Clark et al, , 1991a.…”

Section: Introductionmentioning

confidence: 99%

Accumulation of chromotrope 2R positive cells in equine endometrium during early pregnancy and expression of transforming growth factor- 2 (TGF- 2)

Lea¹,

Stewart²,

Allen³

et al. 1995

Reproduction

Self Cite

View full text Add to dashboard Cite

“…The frequency of recurrent abortion is about 0.5-1% per 100 pregnancy. 9 While the females having pregnancy complications were detected to have thrombophilic defects in a ratio of 49-65%, this frequency was found about 18-22% in normal pregnancies. This make people think that complication risk increases by 3-8 fold, based on thrombophilia.…”

Section: -8mentioning

confidence: 99%