Cytokines/chemokines and immune checkpoint molecules in anti-leucine-rich glioma-inactivated 1 encephalitis

Qiao, Shan; Zhang, Shanchao; Li, Haiyun; Wang, Zhi-Hao; Jin, Yang; Wang, Aihua; Liu, Xuewu

doi:10.1007/s10072-022-06526-6

Cited by 2 publications

(4 citation statements)

References 43 publications

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“…31 MCP1 is required for anti-NMDAR IgG migration to the brain in AE, 32 and MCP1 mRNA expression is elevated in the hippocampus of mice with anti-NMDAR antibody-induced recurrent seizures. 33 Plasma levels of MCP1 were elevated in LGI1-E patients, but not in NMDAR-E or GABABR-E patients 11,34 MCP1 expression was significantly higher in NMDAR-E than in LGI1-E and GABABR-E, but there was no correlation with elevated CSF lymphocyte count and blood-CSF barrier dysfunction. This may be due to the small sample size of NMDAR-E in our cohort for measuring serum MCP1 expression.…”

Section: Discussionmentioning

confidence: 97%

“…The fasting blood samples of seven patients with NMDAR-E were collected on the day following their arrival, while they were still experiencing active disease symptoms, prior to the initiation of immunotherapy, as described in our previous reports. 10,11 These samples were promptly frozen at a temperature of −80°C until they could be analyzed.…”

Section: Sample Collectionmentioning

confidence: 99%

“…The testing was carried out following the manufacturer's instructions, as described in previous studies. 10,11…”

Section: Human Inflammation Molecular Arraymentioning

confidence: 99%

“…Distinct serum cytokines and the association of them with inflammatory CSF signatures in NMDAR-E, LGI1-E and GABABR-E On the basis of the results of our previous studies, 10,11 we investigated whether serum cytokines could serve as inflammatory biomarkers, separating the three AE categories and inferring their underlying pathology, as well as determining their association with CSF inflammatory parameters. To differentiate between NMDAR-E, LGI1-E, and GABABR-E cases, a representative PCA score plot was constructed using cytokine data (Figure S7A).…”

Section: Effect Of Disease Duration On Inflammatory Csf Changes In Nm...mentioning

confidence: 99%

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CSF Findings in Chinese Patients with NMDAR, LGI1 and GABABR Antibody-Associated Encephalitis

Qiao,

Li,

Cui

et al. 2024

JIR

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CSF inflammation in subtypes of antibody-defined autoimmune encephalitis (AE) ranges in intensity from moderate to severe. In a retrospective, cross-sectional study, we characterized CSF findings in Chinese patients with anti-N-methyl-D-aspartate receptor encephalitis (NMDAR-E), anti-leucine-rich glioma-inactivated 1 encephalitis (LGI1-E), and anti-gamma aminobutyric acid-B receptor encephalitis (GABABR-E). Patients and Methods: The AE cases, including 102 NMDAR-E, 68LGI1-E and 15 GABABR-E, were included. CSF inflammatory parameters consisted primarily of CSF leukocytes, oligoclonal bands (OCBs), and CSF/serum albumin ratios (Q Alb ). Ten serum cytokines were evaluated in order to classify AE subtypes. Results: 88% of NMDAR-E, 80% of GABABR-E, and 51% of LGI1-E patients had aberrant CSF features. In NMDAR-E, the CSF leukocyte count, CSF protein concentration, and age-adjusted Q Alb were significantly higher than in LGI1-E, but did not differ from GABABR-E. Blood-CSF barrier dysfunction was less common in NMDAR-E patients with >40 years old. On admission, inflammatory CSF response was more prevalent in NMDAR-E patients with a higher CASE score. With age <60 years, CSF inflammatory changes were less frequent in LGI1-E patients, but more common in GABABR-E patients. MCP-1, IL-10, IL-1β, and IL-4 were potential classifiers for NMDAR-E, LGI1-E, and GABABR-E, and correlated substantially with CSF leukocyte count and Q Alb . Conclusion: Subtype-specific patterns are formed by the various inflammatory CSF parameters in NMDAR-E, LGI1-E, and GABABR-E, and their correlation with disease severity, age, and disease duration. CSF inflammatory characteristics associated with MCP-1, IL-10, IL-1β, and IL-4 may be potential immunopathogeneses targeting markers for these AE subtypes.

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Section: Discussionmentioning

confidence: 97%

Section: Sample Collectionmentioning

confidence: 99%

“…The testing was carried out following the manufacturer's instructions, as described in previous studies. 10,11…”

Section: Human Inflammation Molecular Arraymentioning

confidence: 99%

Section: Effect Of Disease Duration On Inflammatory Csf Changes In Nm...mentioning

confidence: 99%

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CSF Findings in Chinese Patients with NMDAR, LGI1 and GABABR Antibody-Associated Encephalitis

Qiao,

Li,

Cui

et al. 2024

JIR

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Integrated analysis reveals CP is a potential biomarker and therapeutic target in glioma

Liu

Wang

Wang³

et al. 2023

Preprint

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Purpose: Glioma is a challenging brain tumor with limited treatment options and poor prognosis. Ceruloplasmin (CP), a multicopper protein involved in oxidative stress and iron homeostasis, has been implicated in various cancers but its role in glioma remains unclear. Methods: In this study, we investigated the association between CP expression and various factors, including prognosis, tumor microenvironment (TME), immune infiltration, and immune checkpoint markers. We utilized single-cell sequencing data to assess the expression of CP in both glioma tissues and normal tissues. Furthermore, we conducted in vitro experimentation to evaluate the effects of CP knockdown on glioma cells. Results: Our findings demonstrated a significant correlation between high CP expression and unfavorable clinical characteristics, as well as poor overall survival in glioma patients. Enrichment analysis revealed associations between CP and immune-related pathways, suggesting its involvement in immune regulation. We observed increased immune infiltration in glioma cases exhibiting high CP expression. Additionally, CP expression showed positive correlation with immune checkpoint genes, indicating its potential as an immunotherapy target. Single-cell analysis confirmed the expression of CP in both tumor and immune cells, further supporting its role as a biomarker. Moreover, our in vitro knockdown experiments demonstrated that inhibiting CP suppressed glioma cell proliferation. Conclusion: In summary, our findings suggest that CP is associated with glioma progression, immune regulation, and holds potential therapeutic implications.

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Cytokines/chemokines and immune checkpoint molecules in anti-leucine-rich glioma-inactivated 1 encephalitis

Cited by 2 publications

References 43 publications

CSF Findings in Chinese Patients with NMDAR, LGI1 and GABABR Antibody-Associated Encephalitis

CSF Findings in Chinese Patients with NMDAR, LGI1 and GABABR Antibody-Associated Encephalitis

Integrated analysis reveals CP is a potential biomarker and therapeutic target in glioma

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