Cytokines as inducers of postperfusion systemic inflammatory reaction in cardiosurgical patients with different duration of coronary pathology

Chumakova, S. P.; Уразова, О. И.; Шипулин, В. М.; Новицкий, В. В.; Khardikova, S. A.

doi:10.20538/1682-0363-2017-4-260-268

Cited by 7 publications

(4 citation statements)

References 3 publications

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“…Системная воспалительная реакция при ИБС и ХСН характеризуется нарушением баланса про-и противовоспалительных цитокинов [10]. Действительно, содержание IL-4 в крови у обследованных пациентов обеих групп было ниже нормы, а концентрация IL-13 соответствовала таковой.…”

Section: Discussionunclassified

“…Таким образом, сходные патогенетические факторы развития ИБС и ИКМП, сопровождающихся гипоксией и активацией клеток моноцитарно-макрофагального ряда, в сочетании с нарушением продукции как провоспалительных (IL-1β, IL-6, IL-8, TNF-α), так и противовоспалительных (IL-4, IL-10, IL-13) цитокинов [2,10,11], а также трудности диагностики, неблагоприятный прогноз и малоизученный патогенез ИКМП делают актуальным поиск диф-ференциальных диагностических признаков этих двух заболеваний, которые формируют комплекс симптомов хронической сердечной недостаточности.…”

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Interleukin-10 and non-classical monocytes as biomarkers of ischemic cardiomyopathy

Шипулин¹,

Chumakova²,

Pogonchenkova³

et al. 2020

PKiK

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Background. Chronic heart failure frequently occurs against the background of ischemic heart disease (IHD), and has also been associated with the diagnosis of ischemic cardiomyopathy (ICMP). While there are significant commonalities with respect to underlying mechanisms, it would be most important to identify differential diagnostic markers to facilitate diagnosis and verification at the early stages of disease.Aim. The intent of this study was to perform a quantitative assessment of non-classical monocytes in patients diagnosed with IHD with or without ICMP and to identify any relationships between non-classical monocytes and plasma concentrations of proinflammatory (interleukin (IL)-1β, IL-6, tumour necrosis factor (TNF)-α) and anti-inflammatory (IL-4, IL-10, IL-13) cytokines.Methods. We examined 44 patients diagnosed with IHD, aged 49 - 63 years, with class II-III heart failure as per the New York Heart Association criteria. Of this group, 18 were also diagnosed with ICMP. The comparison group included 14 age-matched healthy controls. Percentages of non-classical (CD14+CD16+) monocytes in peripheral blood were determined by flow cytometry and the concentrations of IL-1β, IL-4, IL-6, IL-10, IL-13 and TNF-α were measured by enzyme-linked immunosorbent assay.Results. Amongst the patients diagnosed with ICMP, the concentration of IL-10 in blood plasma was higher than in those without this diagnosis, detected at 30.05 pg/ml (range 24.75 - 33.50 pg/ml, р=0.0412); the percentage of non-classical monocytes was lower, at 5.05% (range 4.08 - 6.58%, р=0.0094). Amongst patients diagnosed with IHD without ICMP, these parameters were within normal limits. The plasma concentrations of IL-1β, IL-6 and IL-13 in both groups were comparable to values obtained from healthy donors and IL-4 was undetectable throughout. Amongst patients diagnosed with ICMP, plasma TNF-α was detected at elevated levels that were comparable to those determined for patients diagnosed with IHD without ICMP.Conclusion. Imbalance of cytokines in blood in patients with coronary artery disease is characterized by excess TNF-α, absence of IL-4, and normal levels of IL-1β, IL-6 and IL-13, regardless of the type of ischemic myocardial injury. In patients with ICMP, we detected an overall decrease in the fraction of non-classical monocytes in association with an increase in plasma IL-10; these features were not detected in patients diagnosed with IHD without ICMP. As such, these biological responses may be used for timely diagnosis of ICMP.Received 27 May 2019. Revised 7 October 2019. Accepted 30 October 2019.Funding: The work is supported by grants of the Russian Foundation for Basic Research No. 18-015-00160\19 and the President of the Russian Federation No. НШ-2690.2018.7 and No. МД-2788.2019.7.Conflict of interest: Authors declare no conflict of interest.Author contributionsConception and study design: V.M. Shipulin, S.P. ChumakovaData collection and analysis: V.M. Shipulin, S.P. Chumakova, O.I. Urazova, M.V. Vins, V.V. NovickiyLiterature review: S.P. Chumakova, D.A. Pogonchenkova, A.S. PryakhinDrafting the article: S.P. Chumakova, D.A. Pogonchenkova, O.I. UrazovaCritical revision of the article: V.M. Shipulin, V.V. NovickiyStatistical analysis: S.P. ChumakovaFinal approval of the version to be published: V.M. Shipulin, S.P. Chumakova, D.A. Pogonchenkova, O.I. Urazova, M.V. Vins, A.S. Pryakhin, V.V. Novickiy

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Section: Discussionunclassified

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Interleukin-10 and non-classical monocytes as biomarkers of ischemic cardiomyopathy

Шипулин¹,

Chumakova²,

Pogonchenkova³

et al. 2020

PKiK

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“…[18]. Известно, что они, также как и PAMPs микроорганизмов, могут распознаваться scavenger-рецепторами моноцитов (макрофагов) и дендритных клеток [1,19], стимулируя синтез провоспалительных цитокинов при ИБС [20]. Считается, что иммунную функцию взаимодействия с Т-лимфоцитами осуществляют промежуточные моноциты [16], в связи с чем, видимо, их численность возрастает у больных ИБС и пациентов с ТБЛ (см.…”

Section: Introductionunclassified

Subpopulations of blood monocytes in patients with generalized hypoxia

et al. 2019

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The aim of the work is to establish general regularities and features of differentiation of blood monocytes into 4 subpopulations in diseases associated with circulatory and respiratory hypoxia.Materials and methods. 18 patients with ischemic heart disease (IHD), 12 patients with ischemic cardiomyopathy (ICMP), 14 patients with chronic obstructive pulmonary disease (COPD), 15 patients with newly diagnosed infiltrative pulmonary tuberculosis (PTB) and 12 healthy donors were examined. In whole blood, we determined the relative number of different subpopulations of monocytes by flow cytometry. The results were analyzed by statistical methods.Results. It is shown that an increase in the number of classical (80.56 [77.60; 83.55]%) and the deficit of intermediate (10.38 [9.36; 11.26]%), non-classical (6.03 [5.24; 6.77]%) and transitional (2.14 [1.41; 3.92] %) monocytes in the blood is determined in patients with COPD when compared with the group of healthy donors (p < 0.05). In groups of patients with PTB and IHD, an increase in the number of intermediate monocytes (26.24 respectively [22.38; 42.88] % and 25.27 [15.78; 31.39]%) and the lack of transitional cells (1.77 [1.36; 3.74]% and 2.68 [2.63; 4.0]%) at the normal content of classical and non-classical forms of monocytes (p < 0.05) is detected. In patients with ICMP, a decrease in the number of non-classical monocytes (up to 5.05 [4.08; 6.58]%) is combined with the normal cell content of other subpopulations (p < 0.05). The interrelation between the number of classical and intermediate monocytes in patients with COPD (r = –0.63; p < 0.05), PTB (r = –0.72; p < 0.01), IHD (r = –0.59; p < 0.05), ICMP (r = –0.58; p < 0.05) was established.Conclusion. In COPD associated with generalized hypoxia, an increase in the number of classical monocytes is combined with a deficiency of their other subpopulations in the blood. In PTB and IHD, antigenic stimulation of the immune system mediates accelerated differentiation of monocytes from classical to intermediate forms with a decrease in the number of transitional cells regardless of the etiology of the disease (infectious or non-infectious) and the type of hypoxia (respiratory or circulatory).

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“…Проведение КШ с ИК является предиктором возникновения более тяжелых осложнений в интра-и постоперационном периодах в сравнении с операцией на РС [1]. Между тем проведение КШ на РС тоже не лишено осложнений, которые обусловлены возникновением воспалительной реакции в ответ на реперфузию миокарда, хирургическую травму и анестезию [2]. Воспалительный ответ, в свою очередь, запускает каскад гуморальных и клеточных реакций, вызывающих дисфункцию эндотелия, и приводит к несостоятельности шунтов, снижению сосудистого сопротивления, гипотонии, нарушению перфузии головного мозга, почек и миокарда [3].…”

Section: Introductionunclassified

Cytokine Profile and Endothelial Status in Patients with Coronary Artery Disease and Different Outcomes of Coronary Artery Bypass Grafting

Шлык

2019

Kuban. nauсh. med. vestnik

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Aim. To assess the cytokine profile and the level of endothelial dysfunction in patients with coronary artery disease (CAD) and different outcomes of coronary artery bypass grafting (CABG).Materials and methods. The study included 95 people, with 22 of them being practically healthy (without CAD). The CAD patients (73) were retrospectively divided into 2 subgroups according to the presence of complications within the first month after the CABG. The first subgroup consisted of 25 patients suffering from complications after CABG, with 48 people making up the second subgroup (no complications after CABG). CABG was performed on a beating heart; bypass grafts were attached during revascularisation. The serum levels of IL-4 and IFN-γ were determined through the enzyme-linked immunosorbent assay (ELISA) using appropriate test systems manufactured by BCM Diagnostics and a Zenyth 340 microplate photometer (Biochrom Ltd). In order to determine the content of endothelin-1 in blood serum, ELISA test systems manufactured by Biomedica were used. The statistical analysis of study results was performed employing the descriptive statistics module of Statistica 12.0 (StatSoft, USA). The mean (M), its error (m), median (Me) and the interquartile range [Q25; Q75] were calculated. In cases when p ≤ 0.05, the results were considered statistically significant.Results. It was revealed that, in the preoperative period, CAD patients from both subgroups exhibited increased levels of endothelin-1 (p = 0.0001) and IFN-γ (p = 0.0001), whereas an increase in IL-4 production was noted only in the first subgroup (p = 0.0001), as compared to the control group. The follow-up revealed that CAD patients with complications after CABG exhibited stable high levels of IL-4 (p > 0.05) and IFN-γ (p > 0.05) during the entire observation period, as compared to the initial values. The content of endothelin-1 in both subgroups increased significantly on the 4th day of observation (p > 0.05) and returned to its initial high values after 1 month.Conclusion. Immune response in CAD patients suffering from complications after CABG develops mainly along the pro-inflammatory pathway and is accompanied by endothelial dysfunction.

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Cytokines as inducers of postperfusion systemic inflammatory reaction in cardiosurgical patients with different duration of coronary pathology

Cited by 7 publications

References 3 publications

Interleukin-10 and non-classical monocytes as biomarkers of ischemic cardiomyopathy

Interleukin-10 and non-classical monocytes as biomarkers of ischemic cardiomyopathy

Subpopulations of blood monocytes in patients with generalized hypoxia

Cytokine Profile and Endothelial Status in Patients with Coronary Artery Disease and Different Outcomes of Coronary Artery Bypass Grafting

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