Cytokines and Particle-Induced Inflammatory Cell Recruitment

Driscoll, Kevin E.; Carter, Janet M.; Hassenbein, Diana G.; Howard, Brian J.

doi:10.2307/3433526

Cited by 44 publications

(36 citation statements)

References 16 publications

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“…We did not detect TNF‐ α in the supernatant of any of our samples. This is in agreement with other work that treated epithelial cells with QM 51. Conversely QM have been shown to trigger release of TNF‐ α in macrophages 50.…”

Section: Discussionsupporting

confidence: 93%

“…Conversely QM have been shown to trigger release of TNF‐ α in macrophages 50. It is also well‐known that pulmonary epithelium can respond to exogenous TNF‐ α produced by macrophages 42, 51. However, the data suggest that human Calu‐3 cells do not produce detectable levels of the cytokine in response to treatment doses used in these experiments.…”

Section: Discussionmentioning

confidence: 81%

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Low Inflammatory Activation by Self‐Assembling Rosette Nanotubes in Human Calu‐3 Pulmonary Epithelial Cells

Journeay

Suri

Moralez

et al. 2008

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Rosette nanotubes (RNT) are a new class of metal-free organic nanotubes synthesized through self-assembly. Because of the wide range of potential biomedical applications associated with these materials, it is necessary to evaluate their potential in vitro toxicity. Here the cytotoxicity of a lysine-functionalized nanotube (RNT-K) in a human Calu-3 pulmonary epithelial cell line is investigated. The cells were treated with media only (control), lysine (50 mg mL(-1)), RNT-K (1, 5, and 50 microg mL(-1)), Min-U-Sil quartz microparticles (QM; 80 microg mL(-1)), and lipopolysaccharide (LPS; 1 microg mL(-1)). The supernatants were analyzed at 1, 6, and 24 h after treatment for the expression of three proinflammatory mediators: IL-8, TNF-alpha and EMAP-II. Cellular viability determined with the Trypan blue assay is significantly reduced in the QM and high-dose RNT-treated groups. TNF-alpha and EMAP-II are undetectable by enzyme-linked-immunosorbent assay (ELISA) in the supernatant of all groups. Although IL-8 concentrations do not differ between treatments, its concentrations increase with time within each of the groups. Quantitative reverse-transcriptase polymerase chain reaction (qRTPCR) of IL-8 mRNA shows increased expression in the high-dose RNT-treated groups at both 1 and 6 h, while an adhesion molecule, ICAM-1 mRNA, shows the greatest increase at 6 h in the QM-treated group. In summary, RNT-K neither reduces cell viability at moderate doses nor does it induce a time-dependent inflammatory response in pulmonary epithelial cells in vitro.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 81%

Low Inflammatory Activation by Self‐Assembling Rosette Nanotubes in Human Calu‐3 Pulmonary Epithelial Cells

Journeay

Suri

Moralez

et al. 2008

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“…TNF‐ α is produced by a number of cell types but upon interaction with bacteria or air pollution particles, the alveolar macrophages are a particularly robust source of this protein 31–33. TNF‐ α is a key initiator of the lung inflammatory cascade as it induces a number of proinflammatory effects including expression of adhesion molecules in other lung cells,32, 33 while EMAP‐II and IL‐8 are potent chemoattractants for monocytes and neutrophils, respectively 34–38. Within 6 h, higher concentrations of secreted TNF‐ α were observed in the LPS‐treated cells relative to all other treatment groups.…”

Section: Discussionmentioning

confidence: 99%

Macrophage Inflammatory Response to Self‐Assembling Rosette Nanotubes

Journeay

Suri

Moralez

et al. 2009

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Rosette nanotubes (RNTs) are a new class of nanomaterials with significant therapeutic potential. However, societal concerns related to the potential adverse health effects of engineered nanomaterials drew attention towards the investigation of their interaction with the human U937 macrophage cell line. The cells are treated with medium only (control), lysine (50 microg mL(-1)), lysine-functionalized RNTs (RNT-K; 1, 5, and 50 microg mL(-1)), Min-U-Sil quartz microparticles (80 microg mL(-1)), or lipopolysaccharide (1 microg mL(-1)). The supernatant and cells are assayed for cell viability, cytokine protein, and mRNA expression at 1, 6, and 24 h post-treatment. The results indicate that RNT-K activate transcription of proinflammatory genes (interleukin-8 and tumor necrosis factor-alpha (TNF-alpha)) within 1 h, but this effect is not accompanied by protein secretion into the supernatant. The effect of the length of RNTs on human U937 macrophage viability is also investigated. Although both short and long RNT-K exhibit time-dependent effects on TNF-alpha transcription, only the short RNT-K (5 microg mL(-1)) increase TNF-alpha concentration at 6 h relative to the long RNT-K. Moreover, RNT-K (1 and 5 microg mL(-1)) have no effect on cell viability by 24 h. These data indicate that RNT-K do not induce a robust inflammatory response or cytotoxicity in the U937 human macrophage cell line, and therefore could be used for biomedical applications.

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“…Interestingly, the mechanism of toxicity associated with the inhalation of silica is unknown. It is proposed, however, that alveolar macrophages ingest the particles, become activated, and release cytokines that recruit inflammatory cells (Driscoll et al , 1997Castronova and Vallyathan 2000;Oberdorster et al 2005a, b). Experimental studies have also shown that crystalline silica <1 μm in diameter resist the normal respiratory defense mechanisms and may remain in the alveoli indefinitely.…”

Section: Introductionmentioning

confidence: 97%

Assessing the in vitro toxicity of the lunar dust environment using respiratory cells exposed to Al2O3 or SiO2 fine dust particles

Jordan

Verhoff

Morgan

et al. 2009

In Vitro Cell.Dev.Biol.-Animal

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Prior chemical and physical analysis of lunar soil suggests a composition of dust particles that may contribute to the development of acute and chronic respiratory disorders. In this study, fine Al(2)O(3) (0.7 μm) and fine SiO(2) (mean 1.6 μm) were used to assess the cellular uptake and cellular toxicity of lunar dust particle analogs. Respiratory cells, murine alveolar macrophages (RAW 264.7) and human type II epithelial (A549), were cultured as the in vitro model system. The phagocytic activity of both cell types using ultrafine (0.1 μm) and fine (0.5 μm) fluorescent polystyrene beads was determined. Following a 6-h exposure, RAW 264.7 cells had extended pseudopods with beads localized in the cytoplasmic region of cells. After 24 h, the macrophage cells were rounded and clumped and lacked pseudopods, which suggest impairment of phagocytosis. A549 cells did not contain beads, and after 24 h, the majority of the beads appeared to primarily coat the surface of the cells. Next, we investigated the cellular response to fine SiO(2) and Al(2)O(3) (up to 5 mg/ml). RAW 264.7 cells exposed to 1.0 mg/ml of fine SiO(2) for 6 h demonstrated pseudopods, cellular damage, apoptosis, and necrosis. A549 cells showed slight toxicity when exposed to fine SiO(2) for the same time and dose. A549 cells had particles clustered on the surface of the cells. Only a higher dose (5.0 mg/ml) of fine SiO(2) resulted in a significant cytotoxicity to A549 cells. Most importantly, both cell types showed minimal cytotoxicity following exposure to fine Al(2)O(3). Overall, this study suggests differential cellular toxicity associated with exposure to fine mineral dust particles.

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Cytokines and Particle-Induced Inflammatory Cell Recruitment

Cited by 44 publications

References 16 publications

Low Inflammatory Activation by Self‐Assembling Rosette Nanotubes in Human Calu‐3 Pulmonary Epithelial Cells

Low Inflammatory Activation by Self‐Assembling Rosette Nanotubes in Human Calu‐3 Pulmonary Epithelial Cells

Macrophage Inflammatory Response to Self‐Assembling Rosette Nanotubes

Assessing the in vitro toxicity of the lunar dust environment using respiratory cells exposed to Al2O3 or SiO2 fine dust particles

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