Cytokine response following perturbation of the cervicovaginal milieu during HPV genital infection

Selinger, Christian; Rahmoun, Massilva; Murall, Carmen Lía; Bernat, Claire; Boué, Vanina; Bonneau, Marine; Graf, Christelle; Grasset, Sophie; Groc, Soraya; Reynes, Jacques; Hirtz, Christophe; Jacobs, Nathalie; Alizon, Samuel

doi:10.1007/s12026-021-09196-2

Cited by 7 publications

(6 citation statements)

References 36 publications

(44 reference statements)

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“…Antibodies were analysed using a multiplex Luminex assay (Waterboer et al, 2005), as already described in the context of HPV infections (Murall et al, 2020). Cytokine data were obtained using MesoScale discovery technology from vaginal secretions collected using ophthalmic sponges placed directly on the cervical os for approximately 1 min, as described in a previous study which analyses this data in the context of HPV infections (Selinger et al, 2021). We used the same protocol to obtain values that were normalized per total protein concentration in the sample.…”

Section: Biological Analysesmentioning

confidence: 99%

Does exposure to different menstrual products affect the vaginal environment?

et al. 2022

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The vaginal ecosystem is a key component of women's health. It also represents an ideal system for ecologists to investigate the consequence of perturbations on species diversity and emerging properties between organizational levels. Here, we study how exposure to different types of menstrual products is linked to microbial, immunological, demographic, and behavioural measurements in a cohort of young adult women who reported using more often tampons (n = 107) or menstrual cups (n = 31). We first found that cup users were older and smoked less than tampon users.When analysing health indicators, we detected potential associations between cups use reporting and fungal genital infection. A multivariate analysis confirmed that in our cohort, reporting using cups over tampons was associated with the higher odds ratio to report a fungal genital infection diagnosis by a medical doctor within the last 3 months. We did not detect significant differences between groups in terms of their | 2593 TESSANDIER et al.

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Section: Biological Analysesmentioning

confidence: 99%

Does exposure to different menstrual products affect the vaginal environment?

et al. 2022

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“…It is widely accepted that HPV requires specific characteristics of the target epithelium for infectivity, including specific tissue receptors including L1 major capsid protein and heparan sulfate proteoglycans of basement membrane 35,36 and site-specific environment inflammation cytokine and macrophage inflammatory proteins. 37 Despite lack of the above specific characteristics in the endometrial cells, HPV DNA has been detected in normal endometrial samples. 10,11 In rare situations, endometrial lesions with koilocytotic squamous atypia, a typical cytopathic evidence of HPV infection have been reported.…”

Section: Discussionmentioning

confidence: 99%

Assisted reproductive technology treatment failure and the detection of intrauterine HPV through spent embryo transfer media sample

Bai,

Sun,

Shu

et al. 2024

Journal of Medical Virology

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Cervical human papillomavirus (HPV) infection is believed to increase the risks of pregnancy failure and abortion, however, whether the uterine cavity HPV infection reduces pregnancy rate or increases miscarriage rate remains unclarified in infertile women undergoing assisted reproductive technology (ART) treatment. Therefore, we aimed to assess ART outcomes in the presence of intrauterine HPV. This was a hospital‐based multicenter (five reproductive medicine centers) matched cohort study. This study involved 4153 infertile women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection treatment in five reproductive medicine centers between October 2018 and 2020. The spent embryo transfer media sample with endometrium tissue were collected and performed with flow‐through hybridization and gene chips to detect HPV DNA. According to basic characteristics, HPV‐positive and negative patients were matched in a ratio of 1:4 by age, body mass index transfer timing, transfer type, and number of embryos transferred. The primary outcome was pregnancy and clinical miscarriage rates in the transfer cycle underwent HPV detection. 92 HPV‐positive and 368 HPV‐negative patients were screened and analyzed statistically. Univariate analysis showed uterine cavity HPV infection resulted in lower rates of ongoing pregnancy (31.5% vs. 44.6%; p = 0.023), implantation (32.3% vs. 43.1%; p = 0.026), biochemical pregnancy (47.8% vs. 62.5%; p = 0.010), and clinical pregnancy (40.2% vs. 54.3%; p = 0.015) compared with HPV negative group. The infertile female with positive HPV also had a slightly higher frequency of biochemical miscarriage (15.9% vs. 13.0%; p = 0.610) and clinical miscarriage (24.3% vs. 15.5%; p = 0.188). These findings suggest that HPV infection in the uterine cavity is a high risk for ART failure. HPV screening is recommended before ART treatment, which may be benefit to improving pregnancy outcome.

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“…Another limitation is that the cytokines were not available for Case B and that those available for Case A were chosen to maximize the detection of HPV-associated trends [16]. Different cytokines, such as IL-6, IL-8, IL-36-γ, IFN-β, TNF-αand chemokines CCL2, CXCL9, CXCL10, could be better suited to capture HSV-2 reactivation events.…”

Section: Discussionmentioning

confidence: 99%

“…To study the local immune response, we collected cervicovaginal secretions using ophthalmic sponges and dosed five cytokines using MesoScale discovery (MSD) technology (see [16] and Supplementary Materials for details). The most striking pattern was an increase in IL-17A between days 94 and 164, i.e.…”

Section: Case Amentioning

confidence: 99%

Concomitant and productive genital infections by HSV-2 and HPV in two young women: a case report

Uysal¹,

Boue²,

Murall³

et al. 2022

Preprint

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Human papillomaviruses (HPVs), the most oncogenic virus known to humans, are often associated with Herpes Simplex Virus-2 (HSV-2) infections. The involvement of the latter in cervical cancer is controversial but its long-term infections might modulate the mucosal microenvironment in a way that favors carcinogenesis. We know little about coinfections between HSV-2 and HPVs, and studying the immunological and microbiological dynamics in the early stages of these infections may help identify or rule out potential interactions. We report two cases of concomitant productive, although asymptomatic, HSV-2 and HPV infections in young women (aged 20 and 25). The women were followed up for approximately a year, with clinical visits every two months and weekly self-samples. We performed quantitative analyses of their HSV-2 and HPV viral loads, immunological responses (IgG and IgM antibodies and local cytokines expression profiles), vaginal microbiota composition, as well as demographic and behavior data. We detect interactions between virus loads, immune response, and the vaginal microbiota, which improve our understanding of HSV-2 and HPVs' coinfections and calls for further investigation with larger cohorts.

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Cytokine response following perturbation of the cervicovaginal milieu during HPV genital infection

Cited by 7 publications

References 36 publications

Does exposure to different menstrual products affect the vaginal environment?

Does exposure to different menstrual products affect the vaginal environment?

Assisted reproductive technology treatment failure and the detection of intrauterine HPV through spent embryo transfer media sample

Concomitant and productive genital infections by HSV-2 and HPV in two young women: a case report

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