Cytokine Regulation of RANTES Production by Human Retinal Pigment Epithelial Cells

Crane, Isabel J.; Kuppner, M. C.; McKillop-Smith, Susan; Knott, Rachel M.; Forrester, John V.

doi:10.1006/cimm.1997.1235

Cited by 44 publications

(29 citation statements)

References 34 publications

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“…Both HRPE and HCHF cells produced significant quantities of CCL-5 and CCL-7 proteins in the presence of low concentrations of inflammatory cytokines (Fig 1 & 4). The secretion of CCL-5 and CCL-7 was also observed in RPE cells prepared from human fetal and adult donor eyes [22,25,47]. Therefore, it is likely that CCL-5 and CCL-7 produced under inflammatory conditions could act as ligands for CCR3 in pathological angiogenesis in CNV.…”

Section: Il-4 Induces Secretion Of Ccl-11 and Ccl-26 But Not Ccl-24 Bmentioning

confidence: 87%

Inflammatory Cytokines Induce Expression of Chemokines by Human Retinal Cells: Role in Chemokine Receptor Mediated Age-related Macular Degeneration

et al. 2015

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Section: Il-4 Induces Secretion Of Ccl-11 and Ccl-26 But Not Ccl-24 Bmentioning

confidence: 87%

Inflammatory Cytokines Induce Expression of Chemokines by Human Retinal Cells: Role in Chemokine Receptor Mediated Age-related Macular Degeneration

et al. 2015

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“…In the last several years, chemokines have been shown to play critical roles in the development of inflammation in diverse disease models (4 -6). Chemokines are produced by ocular cells cultured in vitro, and several chemokines have been detected in ocular fluids and tissues (7)(8)(9)(10)(11)(12)(13)(14). However, a complete analysis of chemokine involvement in the development of ocular inflammation has not been conducted.…”

mentioning

confidence: 99%

Inflammatory Mediators in Uveitis: Differential Induction of Cytokines and Chemokines in Th1- Versus Th2-Mediated Ocular Inflammation

Foxman

Zhang

Hurst³

et al. 2002

The Journal of Immunology

135

116

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Ocular inflammation leads to vision loss through the destruction and scarring of delicate tissues along the visual axis. To identify inflammatory mediators involved in this process, we used real time RT-PCR to quantify the expression of mRNA transcripts of 34 cytokines, 26 chemokines, and 14 chemokine receptors at certain time points during T cell-mediated ocular inflammation. We induced disease by adoptive transfer of Ag-specific Th1 or Th2 cells into recipients expressing the target Ag in their eyes. We also compared the mediator expression patterns seen in adoptive transfer-induced inflammation with that seen in mouse eyes developing experimental autoimmune uveoretinitis. In addition, we used laser capture microdissection to examine chemokine mRNA production by both retinal pigment epithelium cells and infiltrating leukocytes in inflamed eyes. Major findings included the following: 1) Three patterns of expression of the inflammation-related molecules were seen in recipients of adoptively transferred Th cells: preferential expression in Th1 recipients, or in Th2 recipients, or similar expression in both recipient groups. 2) In experimental autoimmune uveoretinitis, the inflammatory mediator expression pattern largely paralleled that seen in Th1-induced disease. 3) Both retinal pigment epithelium and infiltrating leukocytes expressed chemokine transcripts in distinct, but overlapping patterns in inflamed eyes. 4) Interestingly, trancripts of multiple cytokines, chemokines, and chemokine receptors were constitutively expressed in high levels in mouse eyes. Seven of these molecules have not been previously associated with the eye. These data underscore the multiplicity of mediators that participate in the pathogenesis of eye inflammation and point to upstream cytokines as potential therapeutic targets.

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“…Under some conditions, RPE cells may express proteins typical of macrophages and myeloid cells such as Fc receptors, CD68, and inducible NO synthase (1). RPE cells have been shown to produce a wide variety of cytokines in vitro particularly after stimulation with proinflammatory cytokines such as IL-1, TNF-␣, and IFN-␥, including chemokines such as RANTES, IL-8, and monocyte chemoattractant protein-1 (MCP-1) (2)(3)(4).…”

mentioning

confidence: 99%

CXCR4 Receptor Expression on Human Retinal Pigment Epithelial Cells from the Blood-Retina Barrier Leads to Chemokine Secretion and Migration in Response to Stromal Cell-Derived Factor 1α

Crane

Wallace

McKillop-Smith

et al. 2000

The Journal of Immunology

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Retinal pigment epithelial (RPE) cells form part of the blood-retina barrier and have recently been shown to produce various chemokines in response to proinflammatory cytokines. As the scope of chemokine action has been shown to extend beyond the regulation of leukocyte migration, we have investigated the expression of chemokine receptors on RPE cells to determine whether they could be a target for chemokine signaling. RT-PCR analysis indicated that the predominant receptor expressed on RPE cells was CXCR4. The level of CXCR4 mRNA expression, but not cell surface expression, increased on stimulation with IL-1β or TNF-α. CXCR4 protein could be detected on the surface of 16% of the RPE cells using flow cytometry. Calcium mobilization in response to the CXCR4 ligand stromal cell-derived factor 1α (SDF-1α) indicated that the CXCR4 receptors were functional. Incubation with SDF-1α resulted in secretion of monocyte chemoattractant protein-1, IL-8, and growth-related oncogene α. RPE cells also migrated in response to SDF-1α. As SDF-1α expression by RPE cells was detected constitutively, we postulate that SDF-1–CXCR4 interactions may modulate the affects of chronic inflammation and subretinal neovascularization at the RPE site of the blood-retina barrier.

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Cytokine Regulation of RANTES Production by Human Retinal Pigment Epithelial Cells

Cited by 44 publications

References 34 publications

Inflammatory Cytokines Induce Expression of Chemokines by Human Retinal Cells: Role in Chemokine Receptor Mediated Age-related Macular Degeneration

Inflammatory Cytokines Induce Expression of Chemokines by Human Retinal Cells: Role in Chemokine Receptor Mediated Age-related Macular Degeneration

Inflammatory Mediators in Uveitis: Differential Induction of Cytokines and Chemokines in Th1- Versus Th2-Mediated Ocular Inflammation

CXCR4 Receptor Expression on Human Retinal Pigment Epithelial Cells from the Blood-Retina Barrier Leads to Chemokine Secretion and Migration in Response to Stromal Cell-Derived Factor 1α

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