Cytokine profiles of peripheral blood mononuclear cells from dogs experimentally sensitized to Japanese cedar pollen

Fujiwara, Shunsuke; Yasunaga, Sho; Iwabuchi, Shigehiro; Masuda, Kenichi; Ohno, Koichi; Tsujimoto, Hajime

doi:10.1016/s0165-2427(03)00049-7

Cited by 28 publications

(27 citation statements)

References 44 publications

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“…In dogs, the Th2 immune response is present in allergic responses; however, whether this evokes M2 macrophage activation is uncertain. Nevertheless, canine macrophages in allergic reactions are low IFNγ producers and synthesize IL-4 (Fujiwara et al 2003); this makes them similar to mouse M2 macrophages. Moreover, the blunted inflammatory activation of canine macrophages in response to adenosine and ATP (Fujimoto et al 2012) and the increased prostaglandin production in response to ω-3 fatty acids (Kearns et al 1999) resemble features of mouse M2 macrophages.…”

Section: Discussionmentioning

confidence: 99%

Adipose tissue macrophages in non-rodent mammals: a comparative study

et al. 2015

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The stromal vascular fraction (SVF) of adipose tissue in rodents and primates contains mesenchymal stem cells and immune cells. SVF cells have complex metabolic, immune and endocrine functions with biomedical impact. However, in other mammals, the amount of data on SVF stem cells is negligible and whether the SVF hosts immune cells is unknown. In this study, we show that the SVF is rich in immune cells, with a dominance of adipose tissue macrophages (ATMs) in cattle (Bos primigenius taurus), domestic goat (Capra aegagrus hircus), domestic sheep (Ovis aries), domestic cat (Felis catus) and domestic dog (Canis familiaris). ATMs of these species are granulated lysosome-rich cells with lamellipodial protrusions and express the lysosome markers acid phosphatase 5 (ACP-5) and Mac-3/Lamp-2. Using ACP-5 and Mac-3/Lamp-2 as markers, we additionally detected ATMs in other species, such as the domestic horse (Equus ferus caballus), wild boar (Sus scrofa) and red fox (Vulpes vulpes). Feline and canine ATMs also express the murine macrophage marker F4/80 antigen. In the lean condition, the alternative macrophage activation marker CD206 is expressed by feline and canine ATMs and arginase-1 by feline ATMs. Obesity is associated with interleukin-6 and interferon gamma expression and with overt tyrosine nitration in both feline and canine ATMs. This resembles the obesity-induced phenotype switch of murine and human ATMs. Thus, we show, for the first time, that the presence of ATMs is a general trait of mammals. The interaction between the adipose cells and SVF immune cells might be evolutionarily conserved among mammals.

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Section: Discussionmentioning

confidence: 99%

Adipose tissue macrophages in non-rodent mammals: a comparative study

et al. 2015

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“…Total RNA was extracted from the skin samples by a commercially available kit (SV Total RNA Isolation System, Promega, Madison, WI, USA). Transcription of IL‐18 was quantified by one‐step real‐time PCR (TaqMan OneStep RT‐PCR Master Mix Reagents Kit, Applied Biosystems) at the University of Tokyo, using methodologies previously validated 33 . Expression of β‐actin mRNA was used as internal control.…”

Section: Methodsmentioning

confidence: 99%

Cellular and cytokine kinetics after epicutaneous allergen challenge (atopy patch testing) with house dust mites in high‐IgE beagles

Marsella

Olivry

Maeda

2006

Veterinary Dermatology

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The cellular and cytokine dynamics of reactions triggered by atopy patch testing with house dust mites were studied in six high-IgE beagles. Sites were scored and biopsied at 6, 24, 48, and 96 h, and samples were processed for histopathology, immunohistochemistry, and polymerase chain reaction (PCR). All dogs developed positive reactions at some point in time. Mean clinical scores were significantly higher than baseline at 24, 48, and 96 h. Clinically, one of six dogs had a positive reaction at 6 h; two of six reacted at 24 and 48 h, and five of six at 96 h. Histologically, superficial perivascular mononuclear and granulocytic dermatitis developed (5/6) after 6 h, and progressed in severity at 24 h (6/6). Additionally, at 48 h epidermal spongiosis, hyperplasia and pustules developed (5/6), and were marked at 96 h (6/6). At and beyond 6 h, progressive CD1c-positive epidermal Langerhans cell hyperplasia with cluster formation and dermal dendritic cell infiltration was noted. Cutaneous infiltration of CD3-positive T lymphocytes with epidermal clusters developed over time. mRNA expression for the cytokines gamma-interferon (gamma-IFN), interleukin-6 (IL-6), IL-12p35, IL-13, IL-18, and thymus and activation regulated chemokine (TARC) exhibited significant increases during the challenge compared to baseline, but there was no appreciable alteration in expression for tumour necrosis factor-alpha (TNF-alpha), IL-12p40, IL-10, regulated on activation normal T-cell expressed and secreted (RANTES), IL-5, IL-2, IL-4, and IL-8. No correlation was detected between clinical scores and cytokines. It is concluded that IL-6 plays a role in early reactions followed by an increase of TARC and IL-13, while IL-18 progressively increases in later reactions.

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“…The ␤-actin primers and probe sequences (Table 1) were based on canine sequences and were taken from a previously published study (12). The IFN-␥ primers and probe (Table 1) were adapted from a previously published study (12) by substituting bases to match the published badger IFN-␥ gene sequence (7).…”

Section: Methodsmentioning

confidence: 99%

Development and Evaluation of a Test for Tuberculosis in Live European Badgers (Meles meles) Based on Measurement of Gamma Interferon mRNA by Real-Time PCR

et al. 2007

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A real-time PCR assay for the measurement of gamma interferon (IFN-␥) mRNA in European badger (Meles meles) blood cultures was developed. The levels of IFN-␥ mRNA in blood cultures stimulated with either bovine or avian tuberculin or specific mycobacterial antigens were compared with those in a nonstimulated control blood culture as the basis for determining the tuberculosis (TB) status of live badgers. The assay was validated by testing 247 animals for which there were matching data from postmortem examination and culture of tissues. Relative changes in the levels of IFN-␥ mRNA in response to bovine tuberculin and specific antigens were found to be greater among badgers with tissues positive for TB on culture. The test was at its most accurate (87% of test results were correct) by using blood cultures containing bovine tuberculin as the antigen and when the response to avian tuberculin was taken into account by subtracting the avian tuberculin response from the bovine tuberculin response. At a specificity of 90.7%, the test was 70.6% sensitive. At the same specificity, the current serological enzyme-linked immunosorbent assay for TB in badgers was only 53% sensitive. This work demonstrates that measurement of IFN-␥ mRNA by real-time PCR is a valid method for the detection of TB in live badgers and may provide an alternative to the current serological methods of diagnosis, the Brock test. The testing procedure can be completed within 5 h of receipt of the blood culture samples. In addition, the use of a molecular biology-based test offers the potential to fully automate the testing procedure through the use of robotics.There has been a sharp rise in the incidence of bovine tuberculosis (TB) in cattle in the United Kingdom since the early 1990s (1). This adversely affects animal health and welfare and is a cause of considerable economic loss to farmers and the government. The European badger (Meles meles) has been implicated in the spread of disease by acting as a source of Mycobacterium bovis infection in cattle (9, 10). The accurate diagnosis of M. bovis infection in badgers can be achieved by bacteriological culture, but only for samples obtained postmortem. In vitro diagnostics that can be used to test live animals are required, for instance, to further develop vaccination strategies. Antibody-based assays have been developed (13, 14), but they lack sensitivity (3, 14), as they do for cattle (23). In comparison, assays based on measurement of cellular responses produce better results (6, 23). However, those developed to date for badgers are impractical for routine use (6). Tests analogous to that developed for the diagnosis of TB in cattle by the detection of gamma interferon (IFN-␥) in whole-blood cell cultures (21) have yet to be validated for badgers, although the badger IFN-␥ gene has been cloned and a polyclonal antiserum to the cytokine has been produced (7). Having cloned the badger IFN-␥ gene, we investigated the possibility of using IFN-␥ mRNA levels as a measure of the cell-mediated response to myco...

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Cytokine profiles of peripheral blood mononuclear cells from dogs experimentally sensitized to Japanese cedar pollen

Cited by 28 publications

References 44 publications

Adipose tissue macrophages in non-rodent mammals: a comparative study

Adipose tissue macrophages in non-rodent mammals: a comparative study

Cellular and cytokine kinetics after epicutaneous allergen challenge (atopy patch testing) with house dust mites in high‐IgE beagles

Development and Evaluation of a Test for Tuberculosis in Live European Badgers (Meles meles) Based on Measurement of Gamma Interferon mRNA by Real-Time PCR

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