Cytokine Profiles in Immunocompetent Persons Infected withMycobacterium aviumComplex

Abstract

To evaluate the immunologic factors that contribute to protection against Mycobacterium avium complex (MAC), cytokine production by peripheral blood mononuclear cells (PBMC) from human immunodeficiency virus-negative persons with pulmonary MAC (MAC patients) and healthy control subjects with a delayed hypersensitivity skin test response to M. avium sensitin (MAS-positive control subjects) was measured. In MAC patients, mycobacterium-stimulated PBMC produced higher concentrations of interleukin (IL)-10 but lower concentrations of interferon (IFN)-gamma, IL-12, and tumor necrosis factor (TNF)-alpha, compared with PBMC from MAS-positive control subjects. Immunolabeling for intracellular IL-10 revealed that this cytokine was produced by both monocytes and T cells. Alveolar macrophages produced TNF-alpha and IL-10 in response to MAC, which suggests that these cytokines are produced in the lungs of patients with pulmonary disease caused by this pathogen. Our findings suggest that IFN-gamma, TNF-alpha, and IL-12 contribute to protection against MAC, whereas IL-10 is immunosuppressive.

Changes in cholesterol level correlate with the course of pulmonary nontuberculous mycobacterial disease

“…Katalija et al showed that immune-modulating adipokines were abnormally regulated in patients with PNTM disease (23). Several studies reported decreased cytokine production and interferon-γ secretion in patients with PNTM disease (28)(29)(30). In our study, BMI was lower in patients with PNTM disease, in agreement with previous reports.…”

Role of NK Cell-Activating Receptors and Their Ligands in the Lysis of Mononuclear Phagocytes Infected with an Intracellular Bacterium

“…The following Abs were used for immunolabeling: FITC anti-CD14, FITC anti-CD3, PE-conjugated anti-CD56, FITC anti-2B4, and PE-conjugated anti-DNAM-1 (all from BD Pharmingen), and immunostaining was performed by standard techniques (28). In some cases, indirect immunolabeling was performed using mouse mAb to human NKG2D (M585), MICA/B (6D4, as ascites), ULBP1, ULBP2, and ULBP3 (M295, M312, and M551, respectively; Amgen), and mouse polyclonal antisera specific for NKp46 and NKp30 as primary Abs (21) and a FITC goat anti-mouse secondary Ab (Southern Biotechnology Associates).…”

The NKp46 Receptor Contributes to NK Cell Lysis of Mononuclear Phagocytes Infected with an Intracellular Bacterium

“…To measure the percentages of PBMC that expressed CD3 and CD56, we used FITC-conjugated anti-CD3 and PE-conjugated anti-CD56 (both from BD PharMingen, San Diego, CA), and immunostaining was performed by standard techniques (7). Based on forward and side scatter characteristics, we gated on lymphocytes, and the percentages of single-and doublestained cells were determined with an EPICS flow cytometer (Coulter, Hialeah, FL).…”