Cytokine profile in serum and synovial fluid of patients with established rheumatoid arthritis

Moura, Rita A; Cascão, Rita; Perpétuo, I.P.; Canhão, Helena; Sousa, E; Mourão, Ana Filipa; Rodrigues, Ana Maria; Polido-Pereira, Joaquim; Queiróz, M Viana; Rosário, H S; Souto-Carneiro, M M; Graça, Luís; Fonseca, JE

doi:10.1136/ard.2010.129643g

Cited by 3 publications

(2 citation statements)

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“…It is well known that the pro-inflammatory cytokines like interleukins 1 (IL-1), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) are responsible for autoimmunity, chronic inflammation and joint damage [5]. Many pre-clinical and clinical studies revealed elevated level of these cytokines in the serum and synovial fluid in patients with RA [6][7][8]. The cytokines-mediated joint damage occurs by stimulation of mesenchymal cells like synovial fibroblasts, osteoclasts, chondrocytes and thereby release of tissue-destroying matrix metalloproteinases.…”

Section: Introductionmentioning

confidence: 99%

Amelioration of complete Freund’s adjuvant-induced arthritis by Calotropis procera latex in rats

Patel¹,

Kadri

Gohil

et al. 2021

Futur J Pharm Sci

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Background Rheumatoid arthritis is the most common cause of disability, affecting 0.3–1% of the adult population worldwide. The latex of Calotropis procera possesses potent anti-inflammatory as well as analgesic properties. In light above facts, the present study was designed to evaluate anti-arthritic activity of Calotropis procera latex in complete Freund's adjuvant (CFA)-induced arthritis in Wistar albino rats. Complete Freund's adjuvant was injected into the left hind paw on day 0, and treatment of prednisolone and Calotropis procera latex was given from day 0 to 28. Various biochemical, hematological and functional parameters as well as radiological and histopathological changes of joint along with body weight and paw volume were measured. Results Calotropis procera treatment significantly lowered paw volume in CFA-induced arthritic rats. Significant improvement was observed in functional, biochemical and hematological parameters in Calotropis procera-treated rats. However, the body weight remained unaffected. Histological and radiographical examination of synovial joints in Calotropis procera-treated animals exhibited less synovial hyperplasia, infiltration and accumulation of inflammatory cell in synovial fluid, cartilage and bone erosion and joint space narrowing. Conclusion Calotropis procera latex possesses anti-arthritic activity, which is facilitated by modulation in the level of inflammatory mediators and oxidative stress. The improvement in hematological as well as biochemical parameters might be reflected on functional, histopathological, radiological changes and thereby disease progression.

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Section: Introductionmentioning

confidence: 99%

Amelioration of complete Freund’s adjuvant-induced arthritis by Calotropis procera latex in rats

Patel¹,

Kadri

Gohil

et al. 2021

Futur J Pharm Sci

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“…Numerous cytokines have been implicated in the pathogenesis of RA. Levels of IL-17-A, IL-22, IL23, and IL-6 have been identified to be higher in diseased joints than in healthy [74, 75], and IL-6 has been identified to play a key role in the pathogenesis of RA. It is implicated in pathogenesis of local joint disease as well as systemic manifestations [76, 77].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterisation of the synovial fluid microbiome in rheumatoid arthritis patients and healthy control subjects

2019

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The colonisation of specific body sites in contact with the external environment by microorganisms is both well-described and universally accepted, whereas, the existence of microbial evidence in other “classically sterile” locations including the blood, synovial space, and lungs, is a relatively new concept. Increasingly, a role for the microbiome in disease is being considered, and it is therefore necessary to increase our understanding of these. To date, little data support the existence of a “synovial fluid microbiome”.MethodsThe presence and identity of bacterial and fungal DNA in the synovial fluid of rheumatoid arthritis (RA) patients and healthy control subjects was investigated through amplification and sequencing of the bacterial 16S rRNA gene and fungal internal transcribed spacer region 2 respectively. Synovial fluid concentrations of the cytokines IL-6, IL-17A, IL22 and IL-23 were determined by ELISA.ResultsBacterial 16S rRNA genes were detected in 87.5% RA patients, and all healthy control subjects. At the phylum level, the microbiome was predominated by Proteobacteria (Control = 83.5%, RA = 79.3%) and Firmicutes (Control = 16.1%, RA = 20.3%), and to a much lesser extent, Actinobacteria (Control = 0.2%, RA = 0.3%) and Bacteroidetes (Control = 0.1%, RA = 0.1%). Fungal DNA was identified in 75% RA samples, and 88.8% healthy controls. At the phylum level, synovial fluid was predominated by members of the Basidiomycota (Control = 53.9%, RA = 46.9%) and Ascomycota (Control = 35.1%, RA = 50.8%) phyla. Statistical analysis revealed key taxa that were differentially present or abundant dependent on disease status.ConclusionsThis study reports the presence of a synovial fluid microbiome, and determines that this is modulated by disease status (RA) as are other classical microbiome niches.

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Peripheral CD4+ T-cell changes in connective tissue diseases

Vincze

Kolonics-Farkas

Bohács

et al. 2018

Cytokine & Growth Factor Reviews

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Cytokine profile in serum and synovial fluid of patients with established rheumatoid arthritis

Cited by 3 publications

References 0 publications

Amelioration of complete Freund’s adjuvant-induced arthritis by Calotropis procera latex in rats

Amelioration of complete Freund’s adjuvant-induced arthritis by Calotropis procera latex in rats

Molecular characterisation of the synovial fluid microbiome in rheumatoid arthritis patients and healthy control subjects

Peripheral CD4+ T-cell changes in connective tissue diseases

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