Cytokine profile in Nigerians with tubal infertility

Nsonwu-Anyanwu, Augusta Chinyere; Charles-Davies, Mabel Ayebatonyo; Bello, Folasade Adenike; Taiwo, V. O.; Li, Bin; Aa, Oni

doi:10.5114/ceji.2015.56969

Cited by 9 publications

(9 citation statements)

References 33 publications

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“…IL-10 is known to selectively suppress the production of different inflammatory cytokines, whose effects are needed to eradicate C. trachomatis infection [16]. Higher IL-10 levels were seen in infertile woman [17] and other studies found this cytokine to be associated with susceptibility and typical pathological changes caused by the genital Chlamydia infection such as granuloma formation and fibrosis [18]. Higher IL-10 levels in woman may be associated with tubal pathology and may be responsible for their failure to eradicate genital Chlamydia infection and the associated pathology.…”

Section: Discussionmentioning

confidence: 99%

Levels of different cytokines in women and men with asymptomatic genital infection caused by Chlamydia

Bua

Cannas

Zanetti

et al. 2019

J Infect Dev Ctries

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Introduction: Immune response to genital Chlamydia trachomatis infection is involved in both immunity and pathology. The cytokine profile during infection has been implicated in the disease outcome, either resolution or severe sequelae. Methodology: In total, 3900 patients were analyzed for presence of genital infections caused by Chlamydia using molecular assays. Interleukins (IL) IL-10, IL-17, IL-6, IL-2 and chemokine IP-10 were estimated by ELISA in urine, cervical swabs and semen samples. Statistical analysis was performed using the T student test. Results: A total of 47 out of 3900 samples (1.2%) were found to be positive for Chlamydia trachomatis based on the Real Time (RT) PCR results. Statistical analysis revealed that the differences between Chlamydia trachomatis positive and negative samples regarding levels of cytokines were not significant. Conclusions: Our results demonstrated that no significant difference in cytokine concentrations exists in Chlamydia trachomatis infected patients when compared to healthy controls. In further study, we aim to test on a greater number of positive samples a greater number of cytokines involved in the immune response to Chlamydia trachomatis infections.

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Section: Discussionmentioning

confidence: 99%

Levels of different cytokines in women and men with asymptomatic genital infection caused by Chlamydia

Bua

Cannas

Zanetti

et al. 2019

J Infect Dev Ctries

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“…Moreover, N‐nitrosamines caused severe inflammations and consequently increased the levels of cytokines, tumor necrosis factor‐alpha (TNF‐α), interleukin‐1 alpha (IL‐1 α) and interleukin 1 beta (IL‐1 β) cytokines in the male reproductive tract (testis, epididymis and sperm) . Also, Chlamydia positive women with tubal infertility have higher IL‐10 than controls, which may contribute to their development of tubal pathology . Moreover, oxidative stress caused apoptosis in testes and DNA damage in the sperms …”

Section: Discussionmentioning

confidence: 99%

N‐nitrosamines induced infertility and hepatotoxicity in male rabbits

Sheweita

Banna

Balbaa

et al. 2017

Environmental Toxicology

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N-nitrosamines are widely spread environmental pollutants of well-known toxicity and carcinogenicity in various animal species. These compounds are metabolically activated by cytochrome P450 system predominantly in the liver and in other tissues into more active metabolites leading to generation of both alkylating agents that alkylate DNA and reactive oxygen species. In the current study, we investigated the influence of four types of N-nitrosamines that are commonly present in the environment [methyethylnitrosamine, (MEN), diethylnitrosamine (DEN), diphenylnitroasamine (DPN) and dimethylnitrosamine (DMN)] on both livers and testes of male rabbits through assessment of 17 β-hydroxysteroid dehydrogenase (17 β-HSD) activity. The protein expression of the three cytochrome P450s (CYP11A1, CYP19A1, and CYP21A2) is involved in the steroidogenesis. The levels of testosterone (T) and estradiol (E2) were also determined in the plasma of N-nitrosamines-treated rabbits after one, four-, eight- and twelve weeks of treatment of male New Zealand rabbits with an oral dose of 0.5 mg/kg B.W/day of each compound. In addition, activities of glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT) and levels of free radicals measured as thiobarbituric acid reactive substances (TBARS), and reduced glutathione (GSH) level were quantified in both livers and testes. The present study showed that levels of free radicals (TBARS) were markedly increased, whereas GSH levels were depleted in the tissues of both livers and testes after treatment of rabbits with any of N-nitrosamines. In addition, all tested N-nitrosamines inhibited the activities of antioxidant enzyme activities (GR, GST, SOD, and CAT) in hepatic and testicular tissues of rabbits after 12 weeks of treatment. Histopathological examination showed that N-nitrosamines caused lymphocytic infiltration with vascular degeneration and necrosis, congestion of central vein with RBCs hemolysis, dilated sinusoids, as well as fibrosis around portal areas were seen in hepatic tissues. In the testes, histopathological examination displayed disorganized seminiferous tubules with degeneration of germinal epithelium and Sertoli cells. Also, spermatogenic cells had pyknotic nuclei and others were detached from basement membranes of seminiferous tubules, edema was seen between seminiferous tubules. Moreover, the present data showed that MEN and DEN down-regulated the protein expression of both CYP19A1 and 21A2 in both livers and testes of male rabbits. In addition, both MEN and DEN decreased levels of testosterone and estradiol in plasma of treated rabbits. On the one hand, DMN and DPN markedly up-regulated the protein expression of CYP19A1 in both hepatic and testicular tissues of treated rabbits. These compounds potentially increased estradiol and decreased testosterone levels. On the other hand, no correlation was found between the expression of CYP11A1 and levels of both testosterone and estradiol. It is concluded that most of tested N-nitrosamines induce different changes, whic...

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“…In China, approximately 7–10% of couples of childbearing age are confronted with infertility, which is clinically defined as the failure to achieve a pregnancy after one year or more of regular unprotected sexual intercourse [ 1 ]. In recent years, the incidence of fallopian tubal sterility has increased and become one of the most common factors leading to infertility [ 2 ]. At present, the most common methods for investigating tubal patency are laparoscopy and dye laparoscopic chromopertubation, X-ray hysterosalpingography (HSG), and hysterosalpingo-contrast sonography (HyCoSy) [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Influence on Spontaneous Pregnancy of Hysterosalpingo-Contrast Sonography

Gao

Peng

Yan

et al. 2018

BioMed Research International

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Objective Our objective was to explore whether the pregnancy rate (PR) was higher than usual after hysterosalpingo-contrast sonography (HyCoSy). Methods We conducted a prospective observational study of 1,008 infertility patients, all of whom were examined by HyCoSy. The expected time for spontaneous pregnancy was at least 180 days after the HyCoSy exams. There were three types of HyCoSy results: type I, defined as both fallopian tubes patent; type II, defined as one fallopian tube patent with obstruction in the other; and type III, defined as both fallopian tubes obstructed. During the HyCoSy examinations, we recorded the mobility of the ovaries, injective resistance, and contrast agent venous intravasation. Before the examinations, we recorded each patient's medical history, including maternal age, infertility type, median duration of menstrual cycle, dysmenorrhea, and parity number. Results The PR was 19.44% within 180 days after HyCoSy and it was significantly higher in the first 30 days (6.35%) (P <.01). The PR of type I was highest, with a rate of 32.01%, followed by the PR of type II (25.51%) and type III (15.04%) (P <.01). Univariate analysis showed that younger age, patency of both fallopian tubes, good ovarian mobility, and absence of injective resistance were positively related to the initiation of pregnancy (P <.01). Infertility type, median duration of menstrual cycle, dysmenorrhea, parity number, contrast agent venous intravasation, and identity of the sonographer were unrelated to pregnancy (P >.05). However, multivariate analysis showed that patency of both fallopian tubes and the absence of injective resistance were independently associated with pregnancy. Conclusion Some infertility patients conceived successfully and naturally not long after HyCoSy, most often in the first month after the examination. Multivariate analysis showed that patency of both fallopian tubes and the absence of injective resistance were independently factors associated with the ability to conceive after HyCoSy examination.

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Cytokine profile in Nigerians with tubal infertility

Cited by 9 publications

References 33 publications

Levels of different cytokines in women and men with asymptomatic genital infection caused by Chlamydia

Levels of different cytokines in women and men with asymptomatic genital infection caused by Chlamydia

N‐nitrosamines induced infertility and hepatotoxicity in male rabbits

Assessment of the Influence on Spontaneous Pregnancy of Hysterosalpingo-Contrast Sonography

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