Cytokine profile in elderly patients with sepsis

Kumar, Anil; Sudhir, U; K, Punith; Kumar, Rahul; Kumar, Vignesh; Rao, Medha Y

doi:10.4103/0972-5229.56052

Cited by 32 publications

(19 citation statements)

References 25 publications

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“…IL-6 protein level is significantly elevated during acute phase of sepsis (in 4d since sepsis diagnosis) in critically ill patients compared to non-critically ill and healthy ones [ 120 ]. TNF-α level was significantly reduced in non-survivors of elderly patients with sepsis on the first day of diagnosis compared to survivors [ 121 ]. Moreover, both TNF-α and IL-6 levels of septic serum samples were highly increased, and could be used in diagnosis and therapeutic management of neonatal sepsis [ 122 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of interleukin-1 receptor associated kinase-3 (IRAK3) function in modulating expression of inflammatory markers in cell culture models: A systematic review and meta-analysis

et al. 2020

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Background IRAK3 is a critical modulator of inflammation in innate immunity. IRAK3 is associated with many inflammatory diseases, including sepsis, and is required in endotoxin tolerance to maintain homeostasis of inflammation. The impact of IRAK3 on inflammatory markers such as nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in cell culture models remains controversial. Objective To analyse temporal effects of IRAK3 on inflammatory markers after one- or two-challenge interventions in cell culture models. Methods A systematic search was performed to identify in vitro cell studies reporting outcome measures of expression of IRAK3 and inflammatory markers. Meta-analyses were performed where sufficient data were available. Comparisons of outcome measures were performed between different cell lines and human and mouse primary cells. Results The literature search identified 7766 studies for screening. After screening titles, abstracts and full-texts, a total of 89 studies were included in the systematic review. Conclusions The review identifies significant effects of IRAK3 on decreasing NF-κB DNA binding activity in cell lines, TNF-α protein level at intermediate time intervals (4h–15h) in cell lines or at long term intervals (16h–48h) in mouse primary cells following one-challenge. The patterns of TNF-α protein expression in human cell lines and human primary cells in response to one-challenge are more similar than in mouse primary cells. Meta-analyses confirm a negative correlation between IRAK3 and inflammatory cytokine (IL-6 and TNF-α) expression after two-challenges.

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Section: Discussionmentioning

confidence: 99%

Analysis of interleukin-1 receptor associated kinase-3 (IRAK3) function in modulating expression of inflammatory markers in cell culture models: A systematic review and meta-analysis

et al. 2020

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“…Our study has three major strengths. First, unlike similar cross-sectional studies [18] , [49] , [50] , we measured immune markers on ED presentation and during hospital course, and were thus able to compare both the initial immune response in patients at the time of ED presentation and its subsequent trajectory. In most subjects, samples were obtained in the ED prior to initiation of antibiotic therapy and other interventions.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Age on Inflammatory and Coagulation-Fibrinolysis Response in Patients Hospitalized for Pneumonia

et al. 2010

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ObjectiveTo determine whether inflammatory and hemostasis response in patients hospitalized for pneumonia varies by age and whether these differences explain higher mortality in the elderly.MethodsIn an observational cohort of subjects with community-acquired pneumonia (CAP) recruited from emergency departments (ED) in 28 hospitals, we divided subjects into 5 age groups (<50, 51–64, 65–74, 75–84, and ≥85). We measured circulating levels of inflammatory (TNF, IL-6, and IL-10), hemostasis (D-dimer, Factor IX, thrombin-antithrombin complex, antithrombin and plasminogen-activator inhibitor-1), and cell-surface markers (TLR-2, TLR-4, and HLA-DR) during the first week of hospitalization and at discharge and compared 90-day mortality. We used logistic regression to compare odds ratios (OR) for 90-day mortality between age groups, adjusting for differences in pre-infection factors alone and then additionally adjusting for immune markers.ResultsOf 2,183 subjects, 495, 444, 403, 583, and 258 subjects were <50, 51–64, 65–74, 75–84, and ≥85 years of age, respectively. Large age-related differences were observed in 90-day mortality (0.82% vs. 3.2% vs. 6.4% vs. 12.8% vs. 13.6%, p<0.01). No age-related differences in inflammatory and cell surface markers occurred during the first week. Older subjects had higher pro-coagulant markers on ED presentation and over first week (p≤0.03), but these differences were modest (1.0–1.7-fold differences). Odds of death for older adults changed minimally in models incorporating differences in hemostasis and inflammatory markers (for subjects ≥85 compared to those <50, OR = 4.36, when adjusted for pre-infection factors and OR = 3.49 when additionally adjusted for hemostasis markers). At discharge, despite clinical recovery as evidenced by normal vital signs in >85% subjects, older subjects had modestly increased hemostasis markers and IL-6 levels (p<0.01).ConclusionsModest age-related increases in coagulation response occur during hospitalization for CAP; however these differences do not explain the large differences in mortality. Despite clinical recovery, immune resolution may be delayed in older adults at discharge.

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“…For this purpose, immunocompetent cells possess pattern recognition receptors (PRRs) that are capable of recognizing characteristic pathogen associated molecular patterns (PAMPs). Upon interaction of PAMPs and PRRs, adaptor proteins come into play whose task is to activate transcription factors (Kono and Rock, 2008;van der Poll and Opal, 2008;Kumar et al, 2009a). Adaptor proteins activate the transcription factors, including nuclear factor ĸB (NF-ĸB), activation protein-1 (AP-1) and interferon response factor (IRF).…”

Section: Present State Of the Art In The Field -Science And Methodologymentioning

confidence: 99%

Sepsis Diagnostics in the Era of “Omics” Technologies

2018

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Sepsis is a multifactorial clinical syndrome with an extremely dynamic clinical course and with high diverse clinical phenotype. Early diagnosis is crucial for the final clinical outcome. Previous studies have not identified a biomarker for the diagnosis of sepsis which would have sufficient sensitivity and specificity. Identification of the infectious agents or the use of molecular biology, next gene sequencing, has not brought significant benefit for the patient in terms of early diagnosis. Therefore, we are currently searching for biomarkers, through "omics" technologies with sufficient diagnostic specificity and sensitivity, able to predict the clinical course of the disease and the patient response to therapy. Current progress in the use of systems biology technologies brings us hope that by using big data from clinical trials such biomarkers will be found.

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Cytokine profile in elderly patients with sepsis

Cited by 32 publications

References 25 publications

Analysis of interleukin-1 receptor associated kinase-3 (IRAK3) function in modulating expression of inflammatory markers in cell culture models: A systematic review and meta-analysis

Analysis of interleukin-1 receptor associated kinase-3 (IRAK3) function in modulating expression of inflammatory markers in cell culture models: A systematic review and meta-analysis

The Effects of Age on Inflammatory and Coagulation-Fibrinolysis Response in Patients Hospitalized for Pneumonia

Sepsis Diagnostics in the Era of “Omics” Technologies

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