Cytokine Profile in Aqueous Humor of Patients with Ocular Toxocariasis

Abstract

Purpose: Ocular toxocariasis (OT) is a vision-threatening disease with a largely unknown intraocular pathogenesis. Herein, we determined the cytokine expression profile in aqueous humor (AH) of patients with OT.Methods: This is a prospective experimental case-control study of cytokine levels in AH of patients with OT and uveitis and control subjects. Thirty samples from eyes with OT, 23 from eyes with uveitis, and 25 from eyes with age-related cataract were analyzed using a multiplexed magnetic bead immunoassay. Thirty-one cytokines were detected and classified into 5 categories: T-helper type 1 (Th1)-associated cytokines, Th2-associated cytokines, Th17 cytokine, proinflammatory mediators, and growth factors.Results: Higher expression in 19 and decreased expression in 3 cytokines in the OT group were identified, compared with controls. Levels of 17 cytokines were increased in the OT group, compared with the non-OT uveitis and control groups. Levels of 3 cytokines were decreased in the OT group compared with controls; no cytokine level was decreased in the OT and non-OT uveitis groups. Interleukin-10 was the only cytokine showing a significant difference in pairwise comparison among all groups. Th17 levels showed no change, while growth factors and proinflammatory mediators showed some significant changes.Conclusions: We believe this is the first analysis of cytokine expression profile in OT. It revealed that Th2-associated cytokines, especially IL-5, IL-10 and IL-13, present a significantly higher concentration in OT compared with the non-OT uveitis and controls. These cytokines may be important for OT pathogenesis and help identify diagnostic markers and develop treatment strategies in future.

A study on digital twin technology of Human-machine integration cross-belt Sorting System

“…Hu, Tianliang, et al (2021) proposed the concept of a digital twin diagnosis and treatment system, gave the method of establishing a digital twin diagnosis and treatment system, and explored the application in clinical diagnosis and treatment, basic medical research, education and training, and medical dev ice development [10]; Liu (2021) used digital twin to drive a flowing workshop and achieved mutual iterative optimization of the static configuration and dynamic execution mechanism of the system [12]. GURNEY (2020) et al promote the intelligent operation of physical systems through simulation optimization and virtual-real interaction, which has a wide range of applications in intelligent factory construction for smart manufacturing, production workshops, distributed brilliant workshops, sho p floor production processes, and intelligent production management and control [12][13][14][15][16][17][18][19]. Lu Yangguang (2020) studied the digital twin construction method for intelligent manufacturing, which supports the realization of the whole life cycle of the manufacturing industry [20].…”