Cytokine Production by Porcine Mononuclear Leukocytes Stimulated by Mitogens

Rašková, Gabriela; Kovářů, F; Bártová, Jiřina

doi:10.2754/avb200574040521

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…Despite this, their study did not show that pig PBMCs responded to Con A with induced expression of IFNγ, the classical Th1 cytokine. Also in contrast to our data, their transcriptomic analysis failed to show induced IL-10, IL-12 or IFNα expression in response to Con A. Raskova et al, 2005 determined that Con A alone had no stimulatory effect on IFNγ-secreting cells in pig PBMCs relative to unstimulated cells [42]. Together these responses show that PBMC production of cytokines in response to Con A may not be consistent across all species and/or may be highly sensitive to timing of analysis, concentration of Con A, or method of analysis.…”

Section: Influence Of Con a On Cytokine Production From Pbmcscontrasting

confidence: 99%

“…Con A is a T-cell mitogenic lectin that has been used extensively to evaluate lymphocyte activation responses [42,44]. As well as their role in T cell activation, Con A also can bind to and activate monocytes.…”

Section: Influence Of Con a On Cytokine Production From Pbmcsmentioning

confidence: 99%

“…Studies in two-month old pigs showed that the porcine immune system is sufficiently mature to be able to selectively control the response of cytokine producing cells to mitogenic stimulation [42]. In this study, cytokine production from PBMCs and LPMCs was determined in 24 and 15 pigs, respectively.…”

Section: Introductionmentioning

confidence: 96%

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Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model

Pasternak¹,

Ng²,

Käser³

et al. 2014

J Vaccines Vaccin

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Pig peripheral blood-derived mononuclear cells (PBMCs) and lamina propria mononuclear cells (LPMCs) stimulated with mitogens ex vivo can show significant animal-to-animal variation lead to difficulty in interpreting responses in an out-bred animal species. Mixed-cell populations were stimulated ex vivo with 2.5 μg/ml Con A or 2.5 ng/ml PMA plus 250 ng/ml ionomycin (PMAi; (LPCMs only)) or media alone for 72 hours. Supernatants were then tested for cytokine production using a Bioplex assay for porcine IFNα, IFNγ, IL-10, and IL-12. Unstimulated PBMCs had significant levels of IL-10 and the median value for this group decreased in the presence of Con A. Con A did, however, induce production of IFNα and IFNγ, but not IL-12 in this cell population. In contrast, unstimulated and Con A-stimulated LPMCs produced negligible IL-10, IFNα, IFNγ, and the majority of animals' LPMCs showed negligible IL-12 production in response to Con A. In contrast, LPMCs stimulated with PMAi produced IFNγ suggesting cytokine production is mitogen-specific response. When we tracked animal-specific responses, we observed that discrete subsets of animal's PBMCs responded to Con A with significantly increased or decreased IL-10 production relative to unstimulated cells. Further, in the LPMCs, some cells produced no IL-12 in response to Con A but showed augmented production in response to PMAi, while others showed production of IL-12 in response to Con A but no response to PMAi. Flow cytometric analysis showed that the PBMCs were a mixture of CD3+ T cells>CD21+ B cells>CD172+ myeloid cells whereas the LPMCs consisted of mainly Cytotoxic T cells and Natural Killer cells. The percentage of CD8α+CD4+ antigen-experienced T cells was greater in the LPMCs relative to the PBMCs. As expected in an out-bred species, animal-specific differences in cytokine production in response to stimulants exist and may confound interpretation of results unless tracked individually.

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Section: Influence Of Con a On Cytokine Production From Pbmcscontrasting

confidence: 99%

Section: Influence Of Con a On Cytokine Production From Pbmcsmentioning

confidence: 99%

See 1 more Smart Citation

Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model

Pasternak¹,

Ng²,

Käser³

et al. 2014

J Vaccines Vaccin

View full text Add to dashboard Cite

show abstract

“…In addition, low intensity emission lines are also present, as can be seen in the inset of Figure b: a CN band (X 2 ∑ + ←B 2 ∑ + ) at 416.13 nm, an NH band (X 3 ∑ − ←A 3 ∏) at 336.52 nm, and an Ar line (1s 4 ←3p 5 ) at 419.72 nm . The presence of these additional transition lines suggests that PLA solution components (PLA polymer chains and/or solvent molecules) were possibly partially fragmented by the argon plasma jet during PEPT …”

Section: Resultsmentioning

confidence: 99%

Investigation of plasma‐induced chemistry in organic solutions for enhanced electrospun PLA nanofibers

Rezaei

Gorbanev²,

Chys

et al. 2018

Plasma Processes & Polymers

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Electrospinning is a versatile technique for the fabrication of polymer‐based nano/microfibers. Both physical and chemical characteristics of pre‐electrospinning polymer solutions affect the morphology and chemistry of electrospun nanofibers. An atmospheric‐pressure plasma jet has previously been shown to induce physical modifications in polylactic acid (PLA) solutions. This work aims at investigating the plasma‐induced chemistry in organic solutions of PLA, and their effects on the resultant PLA nanofibers. Therefore, very broad range of gas, liquid, and solid (nanofiber) analyzing techniques has been applied. Plasma alters the acidity of the solutions. SEM studies illustrated that complete fiber morphology enhancement only occurred when both PLA and solvent molecules were exposed to pre‐electrospinning plasma treatment. Additionally, the surface chemistry of the PLA nanofibers was mostly preserved.

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“…A 100μl of RPMI-1640 medium was added in negative control well, a 100μl of mix 1(80μl medium + 20μl Pokeweed mitogen (PWM) (Thermo Fisher Scientific Inc., USA) (final conc. of Mix 1 working solution was 2 μg/mL) was added in positive control [22] and a 100μl of mix 2 (80μl medium + 20μl of SARS-CoV-2 Spike RBD (RBM) Peptide Pool (SinoBiological Inc, China) stock solution (final conc. of Mix 2 working solution was 2 μg/mL) [23] was added in test well.…”

Section: Blood Sample Collection and Processingmentioning

confidence: 99%

Robust cellular immune responses to BNT162b2 SARS-CoV-2 mRNA vaccine in T2DM individuals in Bangladesh

Habib

Akhter

Nessa

2022

Preprint

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Background: Diabetes, one of the most common comorbidities of COVID-19, is prioritized for SARS-CoV-2 vaccination. To evaluate the T-cell mediated immune responses, this study assessed and compared IFN-γ and IL-2 secreting PBMCs against the SARS-CoV-2 BNT162b2 mRNA vaccine in individuals with type 2 diabetes mellitus. Methods: In this longitudinal study, 19 known cases of type 2 diabetes and 16 healthy controls were included through purposive sampling and were regularly sampled before 1 dose, 4 weeks after 1 dose and 2-4 weeks after 2dose of vaccination. Ex-vivo ELISpot assays for IFN-γ and IL-2 were done using isolated PBMCs. HbA1c status was determined by an automated analyzer before 1 dose and SARS-CoV-2 spike-specific IgG antibody were assessed by CLIA 4 weeks after 1dose. A p -value <0.05 was considered significant. Results: All participants were seroconverted after 1 dose and showed a robust rise of IFN-γ and IL-2 secreting PBMCs counts in type 2 diabetic participants irrespective of hypertension, gender and age after two doses of vaccination. However, IFN-γ secreting PBMCs counts were significantly lower in type 2 diabetic group than healthy control group after 1 dose. Substantial negative correlations of HbA1c (%) to IFN-γ secreting PBMCs counts after 2 dose were also found in this study. Conclusion: Although type 2 diabetic participants had a lower response initially, BNT162b2 mRNA vaccine overall induces a robust T-cell mediated immune response.

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Cytokine Production by Porcine Mononuclear Leukocytes Stimulated by Mitogens

Cited by 5 publications

References 11 publications

Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model

Grouping Pig-Specific Responses to Mitogen with Similar Responder Animals may Facilitate the Interpretation of Results Obtained in an Out-Bred Animal Model

Investigation of plasma‐induced chemistry in organic solutions for enhanced electrospun PLA nanofibers

Robust cellular immune responses to BNT162b2 SARS-CoV-2 mRNA vaccine in T2DM individuals in Bangladesh

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