Cytokine production by PBMC and serum from allergic and non-allergic subjects following in vitro histamine stimulation to test fexofenadine and osthole anti-allergic properties

Kordulewska, Natalia; Kostyra, Elżbieta; Cieślińska, Anna; Fiedorowicz, Ewa; Jarmołowska, Beata

doi:10.1016/j.ejphar.2016.10.020

Cited by 15 publications

(24 citation statements)

References 69 publications

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“…This herb has a long history of use for asthma in China. Previous studies have indicated that osthole has a variety of pharmacological and biological activities, including anti-allergic, anti-inflammatory, anti-apoptotic, anti-bacterial and antioxidant stress activi-ties ( 24 - 26 ). Additionally, animal studies have demonstrated that osthole can alleviate immune-mediated inflammatory diseases, such as autoimmune encephalomyelitis, IgA nephropathy, and contact dermatitis ( 27 - 29 ).…”

Section: Introductionmentioning

confidence: 99%

Osthole attenuates ovalbumin‑induced lung inflammation via the inhibition of IL‑33/ST2 signaling in asthmatic mice

et al. 2020

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Asthma is a common chronic inflammatory airway disease. Recent studies have reported that interleukin (IL)-33 is a potential link between the airway epithelium and Th2-type inflammatory responses, which are closely related to the progression of asthma. The IL-33 receptor, ST2, is highly expressed in group 2 innate lymphoid cells (ILc2s), Th2 cells, mast cells, eosinophils and natural killer (NK) cells. cnidii Fructus is a chinese herb with a long history of use in the treatment of asthma in china. Osthole is one of the major components of cnidii Fructus. The present study examined the anti-asthmatic effects of osthole in mice and aimed to elucidate the underlying mechanisms involving the IL-33/ST2 pathway. BALB/c mice were sensitized and challenged with ovalbumin and then treated with an intraperitoneal injection of osthole (25 and 50 mg/kg). Subsequently, the airway hyper-responsiveness (AHR) and inflammation of the lungs were evaluated. The amounts of IL-4, IL-5, IL-13, interferon (IFN)-γ and IL-33 in the bronchoalveolar lavage fluid (BALF) were measured by Luminex assay and their mRNA levels in the lungs were measured by reverse transcription-quantitative PcR. The histopathology of the lungs was performed with H&E, PAS and Masson's staining. The expression of ST2 in the lungs was evaluated by immunohistochemistry. The data demonstrated that osthole markedly reduced AHR and decreased the number of eosinophils and lymphocytes in BALF. It was also observed that osthole significantly inhibited the release of Th2-type cytokines (IL-4, IL-5 and IL-13) and upregulated the IFN-γ level in BALF. Moreover, osthole significantly attenuated the IL-33 and ST2 expression in the lungs of asthmatic mice. On the whole, osthole attenuated ovalbumin-induced lung inflammation through the inhibition of IL-33/ST2 signaling in an asthmatic mouse model. These results suggest that osthole is a promising target for the development of an asthma medication.

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Section: Introductionmentioning

confidence: 99%

Osthole attenuates ovalbumin‑induced lung inflammation via the inhibition of IL‑33/ST2 signaling in asthmatic mice

et al. 2020

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“…(3) Kordulewska et al [14] observed this same tendency in cultured PBMCs in allergic adult patients. (4) Stimulation of control PBMC’s using our tested substances produced no significant differences, thus indicating that COX-2 mRNA gene expression is not affected in non-allergic children even after stimulation with inflammatory factors [14]. Finally, our analyses also confirm O’Neill and Ford-Hutchinson’s [27] assertion that COX-2 mRNA gene expression is normally co-expressed at detectable levels in human tissues.…”

Section: Discussionmentioning

confidence: 75%

“…[26] study, where PBMC cells stimulated by LPS, IL-1β, and TNF-α had increased COX-2 expression. (3) Kordulewska et al [14] observed this same tendency in cultured PBMCs in allergic adult patients. (4) Stimulation of control PBMC’s using our tested substances produced no significant differences, thus indicating that COX-2 mRNA gene expression is not affected in non-allergic children even after stimulation with inflammatory factors [14].…”

Section: Discussionmentioning

confidence: 76%

“…Our search for anti-allergic agents from natural sources revealed promising properties in the Cnidium monnieri dried fruit and the isolated substance named osthole, which has an isopentenoxy-coumarin structure. Pharmacological studies demonstrate its wide bioactivity as an anti-osteoporotic, anti-carcinogenic, anti-diabetic, and anti-allergic agent [14,15,16,17]. We hypothesize osthole has potential in allergy treatment in inhibition in COX-2 pathway.…”

Section: Introductionmentioning

confidence: 95%

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High Expression of IL-1RI and EP2 Receptors in the IL-1β/COX-2 Pathway, and a New Alternative to Non-Steroidal Drugs—Osthole in Inhibition COX-2

Kordulewska

Cieślińska

Fiedorowicz

et al. 2019

IJMS

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Background: Osthole (7-methoxy-8-isopentenylcoumarin) is natural coumarin isolated from the fruit of Cnidium monnieri (L.) Cusson, which is commonly used in medical practice of traditional Chinese medicine (TCM) in various diseases including allergies and asthma disorders. Purpose: Osthole was tested for the anti-histamine, anti-allergic, and inhibitory effects of COX-2 (cyclooxygenase-2) in children with diagnosed allergies. Additionally, we hypothesize that stated alterations in children with diagnosed allergies including increased expression of interleukin 1-β receptor type 1 (IL-1 type I) and E-prostanoid (EP) 2 receptors, as well as raised expression, production, and activity of COX-2 and IL-1β in incubated medium are approximately connected. Furthermore, we establish the mechanisms included in the changed regulation of the COX-2 pathway and determine whether osthole may be COX-2 inhibitor in peripheral blood mononuclear cells (PBMCs). Method: PBMCs were obtained from peripheral blood of healthy children (control, n = 28) and patients with diagnosed allergies (allergy, n = 30). Expression of the autocrine loop components regulating PGE2 production and signaling namely IL-1 type I receptor (IL-1RI), cyclooksygenaze-2 (COX-2), E-prostanoid (EP) 2, and also histamine receptor-1 (HRH-1) was assessed at baseline and after stimulation with histamine, osthole, and a mixture of histamine/osthole 1:2 (v/v). This comprised the expression of histamine receptor 1 (HRH-1), IL-1RI, COX-2, EP2 receptor, and the secretion of IL-1β and COX-2 in cultured media and sera. Results: Compared with control group, basal mRNA expression levels of HRH-1, IL-1RI, COX-2, and EP2 were higher in the allergy group. Histamine-induced EP2 and COX-2 expression mRNA levels were also increased. Conclusions: Osthole successively inhibits PGE2 and COX-2 mRNA expression. Furthermore, osthole reduces the secretion of COX-2 protein in signaling cellular mechanisms. Changed EP2 expression in children with allergies provides higher IL-1RI induction, increasing IL-1β capacity to increase COX-2 expression. This effects in higher PGE2 production, which in turn increases its capability to induce IL-1RI.

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“…Specifically, osthole (150 ng/mL) was more effective than fexofenadine in terms of IL-13 blocking ability. The blockage effect on other cytokines such as IL-1β, IL-10, and TNF-α was similar to fexofenadine [70]. The extract of CMC (100 µg/mL) had immune regulatory functions by inhibiting retinoic acid receptor α reporter gene transcription which could affect the immune cell production and ratios [71].…”

Section: Immune Systemmentioning

confidence: 81%

Phytochemistry, Ethnopharmacology, Pharmacokinetics and Toxicology of Cnidium monnieri (L.) Cusson

Sun

Yang

Lenon

2020

IJMS

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Cnidium monnieri (L.) Cusson (CMC) is a traditional Chinese herbal medicine that has been widely grown and used in Asia. It is also known as “She chuang zi” in China (Chinese: 蛇床子), “Jashoshi” in Japan, “Sasangia” in Korea, and “Xa sang tu” in Vietnam. This study aimed to provide an up-to-date review of its phytochemistry, ethnopharmacology, pharmacokinetics, and toxicology. All available information on CMC was collected from the Encyclopedia of Traditional Chinese Medicines, PubMed, EMBASE, ScienceDirect, Scopus, Web of Science, and China Network Knowledge Infrastructure. The updated chemical structures of the compounds are those ones without chemical ID numbers or references from the previous review. A total of 429 chemical constituents have been elucidated and 56 chemical structures have been firstly identified in CMC with traceable evidence. They can be categorized as coumarins, volatile constituents, liposoluble compounds, chromones, monoterpenoid glucosides, terpenoids, glycosides, glucides, and other compounds. CMC has demonstrated impressive potential for the management of various diseases in extensive preclinical research. Since most of the studies are overly concentrated on osthole, more research is needed to investigate other chemical constituents.

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Cytokine production by PBMC and serum from allergic and non-allergic subjects following in vitro histamine stimulation to test fexofenadine and osthole anti-allergic properties

Cited by 15 publications

References 69 publications

Osthole attenuates ovalbumin‑induced lung inflammation via the inhibition of IL‑33/ST2 signaling in asthmatic mice

Osthole attenuates ovalbumin‑induced lung inflammation via the inhibition of IL‑33/ST2 signaling in asthmatic mice

High Expression of IL-1RI and EP2 Receptors in the IL-1β/COX-2 Pathway, and a New Alternative to Non-Steroidal Drugs—Osthole in Inhibition COX-2

Phytochemistry, Ethnopharmacology, Pharmacokinetics and Toxicology of Cnidium monnieri (L.) Cusson

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