Cytokine Production and Cytokine Receptor Expression by Cells of the Human First Trimester Placental–uterine Interface

Jokhi, Percy; King, Ashley; Loke, Y.W.

doi:10.1006/cyto.1996.0146

Cited by 133 publications

(74 citation statements)

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“…The TNF action is known to be mediated through the membrane receptors TNFR1 and TNFR2 expressed in reproductive tissues such as pregnant uterus and placenta [7,8], although TNFR2 protein has been detected mainly in immune cells infiltrated in endometrial decidua [21], similarly to our results where its protein was found in some cells, probably infiltrate leukocytes, in the stromal compartment.…”

Section: Discussionsupporting

confidence: 88%

TNF system in eutopic endometrium from women with endometriosis

Boric¹,

Torres²,

Pinto³

et al. 2013

OJOG

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The role of the tumor necrosis factor (TNF) system in endometriosis is not completely clear and therefore was the focus of this study. In eutopic endometria obtained from women with (n = 41) and without (controls; n = 34) endometriosis during laparoscopy, the subcellular location and the percentages for TNF, TNFR1, TNFR2 and CD45 positive-cells were determined (immunohistochemistry); the protein concentration (ELISA) of the soluble receptors (sTNFR1 and sTNFR2) were measured in 4h-explants culture media and the TNF concentration was performed in ex vivo endometrial homogenates. The TNF, TNFR1 and TNFR2 mRNAs were analyzed by real-time PCR. In control endometria, TNF, TNFR1 and sTNFR1 proteins increased during the late secretory phase. In endometriosis endometria, the protein highest level of TNFR1 was reached during the early and the mid secretory phases and of sTNFR1 concentration during the proliferative phase; however, during the late secretory phase, both TNFR1 and sTNFR1 protein levels were reduced compared to the control endometria (p < 0.05). TNFR2 was scarcely immunodetected in some CD45-positive stromal cells. The TNFand CD45-positive cell percentages, the TNF and sTNFR2 concentrations, and TNF and TNFR2 mRNAs were similar in control and endometriosis endometria. Although TNFR1 mRNA was highly expressed, no significant differences were found between control and endometriosis endometria. In summary, this study establishes that TNF and TNFR1 protein expressions and the sTNFR concentrations in eutopic endometria from women with and without endometriosis are dependent on the menstrual cycle. The differences on the TNFR1 and sTNFR1 protein pattern between both groups, mainly during the mid and late secretory phases may play a role in the lower implantation rates observed in women with endometriosis, and also could be related to the altered cell death, which favor the augmented cell viability facilitating the characteristic endometrial implantation and growth outside the uterus in this disease.

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Section: Discussionsupporting

confidence: 88%

TNF system in eutopic endometrium from women with endometriosis

Boric¹,

Torres²,

Pinto³

et al. 2013

OJOG

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“…Uterine NK cells are a rich source of many different cytokines and growth factors including TNF-α, IL-10, GM-CSF, IL-1β, TGF-β1, CSF-1, LIF and IFN-γ (Saito et al, 1993;Jokhi et al, 1994;Jokhi et al, 1997;Rieger et al, 2001). In general investigation of cytokine production has been on uNK cells purified from early pregnancy decidua, with no separation of decidua basalis and decidua parietalis, although Von Rango et al (2003) compared cytokine production by uNK cells from decidua basalis and decidua parietalis and noted differences in cytokine profile.…”

Section: Functional Investigationsmentioning

confidence: 99%

Immune cells in the placental bed

Bulmer¹,

Williams²,

Lash³

2010

Int. J. Dev. Biol.

311

280

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“…28 Following decidualisation, TNFα mRNA has been shown to be present in macrophages, 29 T-cells, 30 uterine NK cells, endothelial cells, 31 and decidual stromal cells TNFα receptors in pregnancy in vitro. 32,33 Other studies have also shown that various decidual cell types express TNF-Rs. 9 During normal human pregnancy, TNFα gene products have been detected in amniotic fluid [34][35][36][37][38] and soluble TNF-Rs have been detected in first trimester coelomic fluid.…”

Section: Tnfα and Its Receptors During The Normal First Trimestermentioning

confidence: 97%