Cytokine-Mediated Crosstalk Between Keratinocytes and T Cells in Atopic Dermatitis

Humeau, Mélanie; Boniface, Katia; Bodet, Charles

doi:10.3389/fimmu.2022.801579

Cited by 33 publications

(22 citation statements)

References 176 publications

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“…It is well known that the transformations that occur in the skin during senescence [ 3 ] and in some diseases, such as dermatoses [ 3 ], may increase the risk of infections that originate due to a virus [ 10 ], bacteria [ 11 ], and fungi [ 12 ]. In the present study, we demonstrated for the first time that hydrolyzed types I and III collagen importantly inhibited the inflammatory response induced by LPS in human fibroblasts (CCD-1072Sk) and human keratinocytes (hKT-nh-skp-KT0026).…”

Section: Discussionmentioning

confidence: 99%

Hydrolyzed Collagen Induces an Anti-Inflammatory Response That Induces Proliferation of Skin Fibroblast and Keratinocytes

Brandão-Rangel

Oliveira²,

Olimpio³

et al. 2022

Nutrients

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Collagen-based products are found in different pharmaceuticals, medicine, food, and cosmetics products for a wide variety of applications. However, its use to prevent or improve the health of skin is growing dizzyingly. Therefore, this study investigated whether collagen peptides could induce fibroblast and keratinocyte proliferation and activation beyond reducing an inflammatory response induced by lipopolysaccharide (LPS). Human skin fibroblasts (CCD-1072Sk) and human keratinocytes (hKT-nh-skp-KT0026) were seeded at a concentration of 5 × 104 cells/mL. LPS (10 ng/mL) and three doses of collagen peptides (2.5 mg/mL, 5 mg/mL, 10 mg/mL) were used. The readout parameters were cell proliferation; expression of inducible nitric oxide synthase (iNOS); expression of pro-collagen-1α by fibroblasts; and secretion of interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor α (TNF-α), transforming growth factor β (TGF-β), and vascular endothelial growth factor (VEGF) by both cell types. The results demonstrated that all doses of collagen supplementation induced increased proliferation of both human fibroblasts (p < 0.01) and human keratinocytes (p < 0.001), while only the dose of 10 mg/mL induced an increased expression of pro-collagen-1α by fibroblasts. Similarly, only the dose of 10 mg/mL reduced LPS-induced iNOS expression in fibroblasts (p < 0.05) and keratinocytes (p < 0.01). In addition, collagen supplementation reduced the LPS-induced IL-1β (p < 0.05), IL-6 (p < 0.001), IL-8 (p < 0.01), and TNF-α (p < 0.05), and increased the TGF-β and VEGF expression in fibroblasts. Furthermore, collagen supplementation reduced the LPS-induced IL-1β (p < 0.01), IL-6 (p < 0.01), IL-8 (p < 0.01), and TNF-α (p < 0.001), and increased the TGF-β (p < 0.05) and VEGF (p < 0.05) expression in keratinocytes. In conclusion, collagen peptides were found to induce fibroblast and keratinocyte proliferation and pro-collagen-1α expression, involving increased expression of TGF-β and VEGF, as well as the suppression of an inflammatory response induced by LPS.

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Section: Discussionmentioning

confidence: 99%

Hydrolyzed Collagen Induces an Anti-Inflammatory Response That Induces Proliferation of Skin Fibroblast and Keratinocytes

Brandão-Rangel

Oliveira²,

Olimpio³

et al. 2022

Nutrients

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“…AD and psoriasis are systemic and immune-allergic inflammatory skin diseases, the mechanism of which is the dysregulation of immunology ( 30 – 32 ), whereas MSCs play a role on regeneration and more importantly on immunomodulation ( 33 – 35 ). As a result, it could be the new clinical target whether the biological function of MSCs in skin lesion of AD and psoriasis changed and evolved in the pathogenesis of skin inflammatory diseases.…”

Section: The Therapeutic Target Aiming At Lesional Mscs In Ad and Pso...mentioning

confidence: 99%

Therapeutic effects of mesenchymal stem cells and their derivatives in common skin inflammatory diseases: Atopic dermatitis and psoriasis

Yang

Xiao

et al. 2023

Front. Immunol.

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Chronic skin inflammatory diseases including atopic dermatitis (AD) and psoriasis have been considered uncontrolled inflammatory responses, which have usually troubled patients around the world. Moreover, the recent method to treat AD and psoriasis has been based on the inhibition, not regulation, of the abnormal inflammatory response, which can induce a number of side effects and drug resistance in long-term treatment. Mesenchymal stem/stromal cells (MSCs) and their derivatives have been widely used in immune diseases based on their regeneration, differentiation, and immunomodulation with few adverse effects, which makes MSCs a promising treatment for chronic skin inflammatory diseases. As a result, in this review, we aim to systematically discuss the therapeutic effects of various resources of MSCs, the application of preconditioning MSCs and engineering extracellular vesicles (EVs) in AD and psoriasis, and the clinical evaluation of the administration of MSCs and their derivatives, which can provide a comprehensive vision for the application of MSCs and their derivatives in future research and clinical treatment.

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“…Atopic dermatitis (AD) is a complex disease involving epidermal barrier dysfunction and immunological abnormalities 1 . The communication between keratinocytes and T cells as major drivers of immune dysfunction has been studied extensively 2 . Still, there is data available underlining some contribution of dermal fibroblasts to AD pathogenesis 3 .…”

Section: Figurementioning

confidence: 99%

Effects of cultured keratinocyte‐ and fibroblast‐derived mediators on three‐dimensional skin models

Fonfara,

Brodersen,

Suhrkamp

et al. 2023

Clin Experimental Allergy

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To the Editor, Atopic dermatitis (AD) is a complex disease involving epidermal barrier dysfunction and immunological abnormalities. 1 The communication between keratinocytes and T cells as major drivers of immune dysfunction has been studied extensively. 2 Still, there is data available underlining some contribution of dermal fibroblasts to AD pathogenesis. 3 This study therefore aimed to investigate the pathological effects of keratinocyte-and fibroblast-derived factors on 3D skin models focusing on epidermal organization and barrier marker expression.Primary keratinocytes and fibroblasts were cultured and stimulated with AD-relevant cytokines (IL-22, IL-4, IL-13, TNFα) for 24 h.Cells were washed and serum-free keratinocyte differentiation medium (SKDM) was added for 1 h. Supernatant was collected and used for further analyses. Multiplex ELISA (Luminex) was performed to determine protein levels in cell supernatants. For the construction of three-dimensional (3D) skin models, a well-established protocol was used. 4 Stimulation of 3D skin models with cytokines (IL-4, IL-13) or cell supernatants was performed for 48 h. The monoclonal interleukin-4 receptor antagonist dupilumab (dpl) was additionally added for 48 h. Two punch biopsies were taken from each 3D skin and embedded in paraffin for histological analyses and used for RNA isolation and following qPCR. 5 Detailed methods and additional results are available at https:// zenodo. org/ recor ds/ 10036562.

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Cytokine-Mediated Crosstalk Between Keratinocytes and T Cells in Atopic Dermatitis

Cited by 33 publications

References 176 publications

Hydrolyzed Collagen Induces an Anti-Inflammatory Response That Induces Proliferation of Skin Fibroblast and Keratinocytes

Hydrolyzed Collagen Induces an Anti-Inflammatory Response That Induces Proliferation of Skin Fibroblast and Keratinocytes

Therapeutic effects of mesenchymal stem cells and their derivatives in common skin inflammatory diseases: Atopic dermatitis and psoriasis

Effects of cultured keratinocyte‐ and fibroblast‐derived mediators on three‐dimensional skin models

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