Cytokine-induced neutrophil chemoattractant release from hepatocytes is modulated by Kupffer cells

Abstract

To clarify the role of intercellular communication inThe liver lobule contains parenchymal cells (hepatothe liver during accumulation of neutrophils, the re-cytes) and four types of nonparenchymal cells, i.e., In severe acute alcoholic hepatitis, the plasma levels was measured by western blotting analysis and enzyme-of endotoxin and several kinds of cytokines such as linked immunosorbent assay (ELISA), and expression of interleukin 1 (IL-1), tumor necrosis factor a (TNF-a), its messenger RNA (mRNA) was assessed by the poly-and IL-8 are reported to be increased. [4][5][6][7] Neutrophil merase chain reaction. LPS-stimulated Kupffer cell-accumulation observed around sites of hepatocyte ne- kinds of cytokines and toxic substances like oxygenfree radicals and reactive nitrogen. 11,12 Previous studies have shown that endotoxin administration induces hepatic injury in alcohol-fed rats 13Abbreviations: IL, interleukin; TNF-a, tumor necrosis factor a; CINC, cytokine-induced neutrophil chemoattractant; LPS, lipopolysaccharide; ELISA, enand that endotoxemia is common in patients with alco- CINC production by hepatocytes in vitro was analyzed

Na+/H+ exchanger inhibitor, FR183998, has protective effect in lethal acute liver failure and prevents iNOS induction in rats

“…During endotoxemia, pro-inflammatory cytokines, such as TNF-a, IL-1b, IL-6 and interferonc, are considered to play an important role in the injury to multiple organs in addition to the liver [43]. Moreover, CINC-1 is the most potent chemokine for neutrophils in rats [44]. TNF-a and CINC-1 are produced by Kupffer cells in the liver under septic conditions [45] and ischemia-reperfusion [46].…”

Protective Effect of Neutrophil Elastase Inhibitor (FR136706) in Lethal Acute Liver Failure Induced by D-Galactosamine and Lipopolysaccharide in Rats

“…TNF-␣ is a key mediator of the cytokine cascade in sepsis [27,28]. Moreover, CINC-1 is a member of the IL-8 family and is the most potent chemokine for neutrophils in rats [29]. CINC-1 is produced by inflammatory cells such as Kupffer cells, which are residential macrophages in the liver, under septic conditions [30] and ischemiareperfusion [31].…”

Free radical scavenger (edaravone) prevents endotoxin-induced liver injury after partial hepatectomy in rats

“…TNF-a is a key mediator of the cytokine cascade in sepsis [7,38]. Moreover, in rats, CINC, which is a member of the IL-8 family, is the most potent chemokine for neutrophils [39] and is produced by inflammatory cells such as Kupffer cells in septic conditions [10]. Therefore, inhibiting the release of these mediators is important in anti-inflammatory therapy.…”