Cytokine GM‐CSF Genetic Adjuvant Facilitates Prophylactic DNA Vaccine against Pseudorabies Virus through Enhanced Immune Responses

Yoon, Hyun Ah; Aleyas, Abi George; George, Junu A.; Park, Seong Ok; Han, Young Soo; Lee, John Hwa; Cho, Jeong Gon; Eo, Seong Kug

doi:10.1111/j.1348-0421.2006.tb03773.x

Cited by 37 publications

(23 citation statements)

References 48 publications

(48 reference statements)

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“…Although GM-CSF has been shown to have adjuvant effects in mice (Lu et al, 2002; Wen et al, 2010; Yoon et al, 2006; Zhang et al, 2011), monkey (Loudon et al, 2010) and human studies (Jaffee et al, 2001; Luiten et al, 2005; Mastrangelo et al, 1999), there is evidence of a counteracting role in immune suppression (Kass et al, 2000). The disparate effects of GM-CSF partly depend on whether it is expressed locally at the site of immunization or systemically (Parmiani et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Since then other studies have shown the efficacy of GM-CSF as a vaccine adjuvant in mice (Lu et al, 2002; Wen et al, 2010; Yoon et al, 2006; Zhang et al, 2011) and NHP (Loudon et al, 2010). The use of GM-CSF has even extended to human clinical trials in which it has been used as an efficient adjuvant in cancer vaccines (Jaffee et al, 2001; Luiten et al, 2005; Mastrangelo et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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A role for granulocyte–macrophage colony-stimulating factor in the regulation of CD8+ T cell responses to rabies virus

et al. 2012

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Inflammatory cytokines have a significant role in altering the innate and adaptive arms of immune responses. Here, we analyzed the effect of GM-CSF on a RABV-vaccine vector co-expressing HIV-1 Gag. To this end, we immunized mice with RABV expressing HIV-1 Gag and GM-CSF and analyzed the primary and recall CD8+ T cell responses. We observed a statistically significant increase in antigen presenting cells (APCs) in the spleen and draining lymph nodes in response to GM-CSF. Despite the increase in APCs, the primary and memory anti HIV-1 CD8+ T cell response was significantly lower. This was partly likely due to lower levels of proliferation in the spleen. Animals treated with GM-CSF neutralizing antibodies restored the CD8+ T cell response. These data define a role of GM-CSF expression, in the regulation of the CD8+ T cell immune responses against RABV and has implications in the use of GM-CSF as a molecular adjuvant in vaccine development.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A role for granulocyte–macrophage colony-stimulating factor in the regulation of CD8+ T cell responses to rabies virus

et al. 2012

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“…However, both antibody‐ and cell‐mediated immunity might play a role in protection. In reality, both enhanced humoral and cellular immunity provided effective protection against a virulent PrV challenge 17 . Furthermore, it is clear that antibodies protect against mortality after a PrV challenge 41,42 .…”

Section: Discussionmentioning

confidence: 99%

Differential polarization of immune responses by genetic cotransfer of chemokines changes the protective immunity of DNA vaccine against pseudorabies virus

Yoon

2006

Immunology

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SummaryChemokines play a key role in eliciting adaptive immune responses by selectively attracting the innate cellular components to the site of antigen presentation. To evaluate the effect of the genetic adjuvant of chemokines on the adaptive immune responses induced by a plasmid DNA vaccine expressing glycorotein B (gB) of the pseudorabies virus (PrV), a PrV DNA vaccine was co-inoculated with plasmid DNA expressing certain chemokines including CCL3 (MIP-1a), CCL4 (MIP-1b), CCL5 (RANTES), CXCL8 (MIP-2), and CXCL10 (IP-10). A co-injection of the CCL3 plasmid DNA induced immunity that was biased to the T helper type 2 (Th2) pattern, as judged by the ratio of immunoglobulin G isotypes and the production of interleukin-4 cytokine generated from stimulated immune T cells. However, CCL5 and CXCL10 induced immune responses of the Th1-type, which rendered the recipients more resistant to a virulent virus infection. CXCL8 also showed enhanced humoral and cell-mediated immunity (mixed-type pattern) providing effective protection against a viral challenge. However, there was no change in the immune responses induced by the PrV DNA vaccine in CCL4 recipients. These results suggest that co-injection of a chemokine, in the form of an adjuvant preparation, causes a rebalancing of the immunity, which subsequently affects the protective efficacy against a virulent virus infection.

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“…Consequently, recombinant pertussis DNA vaccine is also valuable to prevent the infection of the pathogen. Several studies also showed that coimmunization with pGM-CSF enhanced the protection ratio of DNA vaccine (27,34). However, other studies showed GM-CSF did not improve protection (12,32).…”

Section: Discussionmentioning

confidence: 99%

Protective Immunity against Bordetella pertussis by a Recombinant DNA Vaccine and the Effect of Coinjection with a Granulocyte‐Macrophage Colony Stimulating Factor Gene

Zhu

Chu

et al. 2006

Microbiology and Immunology

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A recombinant pertussis DNA vaccine was described here with its immunogenicity and the ability to induce protection against B. pertussis infection in mice. Three immunodominant antigen gene fragments of pertussis, pertussis toxin subunit 1 (pts1), fragments of pertactin (prn) and filamentous hemagglutinin (fha), were recombined as fragment pts1‐prn‐fha named ppf, and it was cloned to plasmid pVAX1 as pVAX1/ppf. Compared to those injected with pVAX1, the mice injected with pVAX1/ppf significantly elicited more antigen specific antibody anti‐PTS1, anti‐PRN, anti‐FHA and cytokine IL‐10, IFN‐γ. When pGM‐CSF was coinjected with pVAX1/ppf, the mice showed significantly increases of the three antibodies and cytokine IL‐10, IL‐4, IFN‐γ and TNF‐α compared to those injected with pVAX1 only. The mice in group pVAX1/ppf & pGM‐CSF, in particular; induced much more anti‐PTS1, IL‐4 and TNF‐α than those in group pVAX1/ppf. In the intracerebral mouse protection test, the mice immunized with pVAX1/ppf or pVAX1/ppf & pGM‐CSF induced protection to a lethal dose of B. pertussis. The results indicate that recombinant DNA vaccine and pGM‐CSF coinjection can induce protective immunity against B. pertussis, demonstrating a valuable method to prevent pertussis.

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Cytokine GM‐CSF Genetic Adjuvant Facilitates Prophylactic DNA Vaccine against Pseudorabies Virus through Enhanced Immune Responses

Cited by 37 publications

References 48 publications

A role for granulocyte–macrophage colony-stimulating factor in the regulation of CD8+ T cell responses to rabies virus

A role for granulocyte–macrophage colony-stimulating factor in the regulation of CD8+ T cell responses to rabies virus

Differential polarization of immune responses by genetic cotransfer of chemokines changes the protective immunity of DNA vaccine against pseudorabies virus

Protective Immunity against Bordetella pertussis by a Recombinant DNA Vaccine and the Effect of Coinjection with a Granulocyte‐Macrophage Colony Stimulating Factor Gene

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