Cytokine Gene Expression in Intact Anagen Rat Hair Follicles

Little, J; Westgate, Gillian; Evans, Anita; Granger, Stewart

doi:10.1111/1523-1747.ep12398584

Cited by 29 publications

(27 citation statements)

References 36 publications

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“…IGF-I has been shown to be expressed at high levels in hair follicles during the growth phase of the hair cycle (anagen) [39], and plays a critical role in regulation of the growth and function of melanocytes [40].…”

Section: Discussionmentioning

confidence: 99%

Dietary isoflavone increases insulin-like growth factor-I production, thereby promoting hair growth in mice

Zhao

Harada

Kurihara

et al. 2011

The Journal of Nutritional Biochemistry

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Section: Discussionmentioning

confidence: 99%

Dietary isoflavone increases insulin-like growth factor-I production, thereby promoting hair growth in mice

Zhao

Harada

Kurihara

et al. 2011

The Journal of Nutritional Biochemistry

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“…cDNA was synthesized from 5 g of total RNA in a reaction mixture of 50 l containing 0.5 g of oligo15dT primer (Promega, Austin, TX), 0.4 mM dNTP (Ecogen, Barcelona, Spain), 10 mM dithiothreitol, and 10 U of Moloney murine leukemia virus (both of Epicenter, Madison, WI). The resultant cDNA was amplified in a total volume of 50 l with 1 U of TaqDNA and 0.2 mM dNTP mixture, and 0.4 M of NGF primers (Little et al, 1994) generating a fragment of 288 pb. As a control of the efficiency of cDNA synthesis we amplified GAPDH (upper primer 5Ј-ccgccccttccgctgatgcc-3Ј and lower primer 5Ј-atgagcccttccacgatgcc-3Ј), generating a fragment of 140 pb to test the efficiency of cDNA synthesis.…”

Section: Methodsmentioning

confidence: 99%

Antinerve Growth Factor Treatment Prevents Intestinal Dysmotility inTrichinella spiralis-Infected Rats

Torrents

Torres²,

Mora³

et al. 2002

J Pharmacol Exp Ther

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Nerve growth factor (NGF) could be involved in the development of hyperalgesia as well as in nervous remodeling consequence of inflammation. Both dysmotility and increase of visceral sensitivity have been described in functional gastrointestinal disorders such as irritable bowel syndrome. Trichinella spiralis-infected rats show an exacerbated spontaneous motility and a significant increase of the excitatory response to cholecystokinin (CCK), both associated with a reversible inflammatory process and the hypertrophy of the muscle layers. In this study we determined the intestinal expression of NGF mRNA by polymerase chain reaction and NGF by enzyme-linked immunosorbent assay. We implanted serosal strain gauge transducers on duodenum, jejunum, and ileum of anesthetized Sprague-Dawley rats to record circular muscle contractions. The experimental protocol included the evaluation of intestinal spontaneous motor activity (SMA), the response to CCK-8, and the ascending contraction induced by electrical mucosal stimulation. This protocol was performed in healthy and infected nontreated rats, in healthy rats with an NGF antibody treatment (1.6 mg/rat i.p.), and in infected rats with the same treatment applied at 0 or 3 days postinfection. NGF and NGF mRNA levels in the bowel were increased during inflammation. Although anti-NGF treatments did not prevent or reverse inflammatory response, the treatment was effective in preventing the motor alterations induced by the T. spiralis infection, i.e., inhibited increased SMA, reversed altered response to CCK, and reversed in part exacerbated response to electrical stimulation.

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“…Several groups have extracted RNA from rodent and human hair follicles [24,[28][29][30][31][32][33][34]. In most cases, the hair samples were stored in dissolution buffers, such as RNA later [24] or RN easy [28], prior to the analysis.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of the human hair roots as a tool for spaceflight experiments

Terada¹,

Seki²,

Higashibata³

et al. 2013

ABB

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The use of hair roots as experimental samples has been a research focus for understanding the effects of spaceflight on astronauts, because it has many advantages, one of which is the fact that hair matrix cells actively divide in a hair follicle and sensitively reflect the physical conditions of the human body. In 2009, a research program focusing on the analysis of astronauts' hairs was initiated to examine the effects of long-term spaceflight on the gene expression and mineral metabolism in the human body. Since the number of samples per astronaut is limited to 5 strands of hairs at each sampling point, due to the ethical viewpoint of astronauts or limited resources in space, it is important to develop an effective method for the molecular analysis of small amounts of hair roots. In this study, mRNA successfully extracted from 1, 5, and 10 hair follicles was amplified and subjected to the DNA microarray analysis to compare the gene expression within subjects. The results indicated that (1) it was possible to perform the genetic analysis on hair samples stored at −80˚C, even without a fixation buffer and (2) the newly modified method of mRNA extraction and analysis was effective in detecting differential gene expression in samples containing only 5 hairs. In conclusion, RNA was efficiently extracted from 5 hair roots, which is the same number of hair roots used in the space experiment; therefore, this method can be applied to genetically analyze astronauts' hair amples. s

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Cytokine Gene Expression in Intact Anagen Rat Hair Follicles

Cited by 29 publications

References 36 publications

Dietary isoflavone increases insulin-like growth factor-I production, thereby promoting hair growth in mice

Dietary isoflavone increases insulin-like growth factor-I production, thereby promoting hair growth in mice

Antinerve Growth Factor Treatment Prevents Intestinal Dysmotility inTrichinella spiralis-Infected Rats

Genetic analysis of the human hair roots as a tool for spaceflight experiments

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