Cytokine expression profiles in cervical mucus from patients with cervical cancer and its precursor lesions

Otani, Shinichi; Fujii, Takuma; Kukimoto, Iwao; Yamamoto, Naoki; Tsukamoto, Tetsuya; Ichikawa, Ryoko; Nishio, Eiji; Iwata, Aya

doi:10.1016/j.cyto.2019.05.011

Cited by 24 publications

(30 citation statements)

References 26 publications

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“…Most studies on local immune response to HPV collect cervical mucus for cytokine measurements using either an endocervical brush or a cervicovaginal lavage [8][9][10][11][12][13][14][15][16], which essentially measures the spillover from cells located at the surface epithelium into the luminal fluid [17]. However, cells in other locations also release many cytokines involved in the immune response to infection.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of IL-2 and IL-23 in Cervical Biopsies of Cervical Intraepithelial Lesions: A Cross-Sectional Study

et al. 2020

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Introduction: Persistent infection with high-risk human papillomavirus (HPV) types is associated with high-grade intraepithelial lesions (HSILs) and invasive cervical cancer. The host immune response plays a key role in whether HPV clears or persists. Most studies on local immune response to HPV collect cervical mucus in order to quantify secreted cytokines; however, cells located inside the tissue can release different cytokines associated with HPV infection. Objective: This study compared the cytokine levels in cervical biopsy specimens of women with abnormal colposcopic findings according to the histopathological results: low-grade intraepithelial lesion (LSIL), HSIL, and no intraepithelial lesion (NSIL). Methods: A cross-sectional study enrolling 141 cervical biopsy specimens examined the cytokine profile for interleukin (IL-) 2, IL-4, IL-10, IL-12, IL-17, and IL-23 and interferon-γ, using the Luminex assay/ELISA. Differences in cytokine levels among the cervical lesion groups were assessed using the Kruskal-Wallis test. Results: The 141 specimens included 90 HSILs, 22 LSILs, and 29 NSILs. IL-2 levels were significantly higher in NSIL samples than in LSIL or in HSIL samples (p = 0.0001) and IL-23 levels were significantly higher in NSIL than in HSIL samples (p = 0.003). Conclusions: Our study shows that in samples from the lesion site point, 2 important pro-inflammatory cytokines, IL-2 and IL-23, are downregulated in HPV lesions.

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Section: Introductionmentioning

confidence: 99%

Downregulation of IL-2 and IL-23 in Cervical Biopsies of Cervical Intraepithelial Lesions: A Cross-Sectional Study

et al. 2020

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“…Presume-se que a ativação de citocinas antivirais se inicie logo após o estabelecimento da infecção pelo HPV (SCOTT et al, 2013). No entanto, a associação entre a quantificação de citocinas e a gravidade da lesão cervical é pouco compreendida, pois existem vários fatores que podem influenciar na ativação destas substâncias que precisam ser mais bem conhecidos, como a história natural da infecção pelo HPV e a resposta imune específica do hospedeiro (OTANI et al, 2019).…”

Section: Discussionunclassified

“…Vários estudos correlacionaram o nível de citocinas com lesões cervicais e infecção pelo HPV (BAIS et al, 2007;KOBAYASHI et al, 2008;PEGHINI et al, 2012;KOSHIOL et al, 2014;HARDIKAR et al, 2015;IWATA et al, 2015;DANIILIDIS et al, 2016;OTANI et al, 2019 (BAIS et al, 2007). Esta citocina é uma glicoproteína expressa por linfócitos B, células dendríticas e fagócitos, e aparenta ter um efeito protetor contra o câncer de colo uterino (PARADKAR et al, 2014).…”

Section: Discussionunclassified

“…A infecção pelo HPV de alto risco tem a capacidade de interferir nas vias de sinalização do sistema imune inato e adaptativo, interferindo diretamente na produção de citocinas (TUMMERS; BURG, 2015). Para demonstrar este efeito, diversos estudos avaliaram a concentração de citocinas no colo uterino em relação ao status de infecção pelo HPV, independente da lesão associada (TELESHEVA et al, 2012;LI et al, 2019;OTANI et al, 2019 como seu organismo responde a infecção que o papiloma vírus humano causa. Esta avaliação será realizada de duas formas: pela dosagem no seu sangue de substâncias envolvidas nas defesas do organismo humano.…”

Section: Discussionunclassified

“…A maioria dos estudos que avaliou a imunidade no colo uterino em mulheres com lesões intraepiteliais cervicais induzidas pelo HPV realizou a quantificação de citocinas no muco cervical ou lavado cervicovaginal (CASTLE et al, 2002;MARKS et al, 2011;TELESHEVA et al, 2012;SCOTT et al, 2013;EKALAKSANANAN et al, 2014;IWATA et al, 2015;DANIILIDIS et al, 2016;LI et al, 2019;OTANI et al, 2019). No entanto, muitas citocinas são expressas a partir de células específicas que não estão localizadas na superfície do epitélio e a avaliação da secreção cervicovaginal estaria medindo apenas o transbordamento destas substâncias para o lúmen vaginal.…”

Section: Agcunclassified

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Perfil intralesional de citocinas em lesões intraepiteliais de baixo e alto grau do colo uterino

Petrini¹

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Changes in cervical cytokine profiles following focused ultrasound treatment for high‐risk human papillomavirus infection–related low‐grade cervical lesions

Wang

Zhou

Liu

et al. 2023

Intl J Gynecology & Obste

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ObjectiveTo evaluate the changes of cervical soluble immune markers after focused ultrasound (FU) treatment to explore the underlying local immune effects of FU in the treatment of high‐risk human papillomavirus (HR‐HPV) infection–related low‐grade squamous intraepithelial lesion (LSIL).MethodsA total of 35 patients diagnosed with HR‐HPV infection–related histological LSIL who met the inclusion criteria were enrolled in this prospective study and treated with FU. The authors used cytometric bead array to measure T‐helper type 1 (Th1) cytokine (interleukin [IL] 2, tumor necrosis factor, and interferon γ) and Th2 cytokine (IL‐4, IL‐5, IL‐6, and IL‐10) levels in the cervicovaginal lavage of patients before and 3 months after FU treatment.ResultsAfter FU treatment, the concentrations of Th2 cytokines IL‐5 and IL‐6 were significantly lower than those before FU treatment (P = 0.044 and P = 0.028, respectively). HR‐HPV infection was cleared in 27 patients, with a clearance rate of 77.1% (27 of 35). The concentration of IL‐4 in patients with HR‐HPV clearance after FU treatment was significantly lower than that in patients without HR‐HPV clearance (P = 0.045).ConclusionFU can inhibit the production of certain Th2 cytokines and may improve the local immune status of the cervix, thereby eliminating HR‐HPV infection.

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Cytokine expression profiles in cervical mucus from patients with cervical cancer and its precursor lesions

Cited by 24 publications

References 26 publications

Downregulation of IL-2 and IL-23 in Cervical Biopsies of Cervical Intraepithelial Lesions: A Cross-Sectional Study

Downregulation of IL-2 and IL-23 in Cervical Biopsies of Cervical Intraepithelial Lesions: A Cross-Sectional Study

Perfil intralesional de citocinas em lesões intraepiteliais de baixo e alto grau do colo uterino

Changes in cervical cytokine profiles following focused ultrasound treatment for high‐risk human papillomavirus infection–related low‐grade cervical lesions

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