Cytokine expression in peripheral blood lymphocytes indicates a switch to Thelper cells in patients with preeclampsia

Host Inflammatory Response Profiling in Preeclampsia Using anIn VitroWhole Blood Stimulation Model

“…Baseline intracellular expression of pro-inflammatory IL-2 in peripheral blood mononuclear cells is increased in PET, as is its production in response to phytohaemagglutinin compared to healthy controls (19,33). This observation is supported by the increased IL-2 secretory capacity associated with PET in the present study.…”

The role of the immune system in preeclampsia

“…We firstly reported that the population of Th1 type cells increased, while that of Th2 type cells decreased, in preeclmapsia compared to those in healthy pregnancy (Saito et al, 1999b). The same results were reported later (Yoneyama et al, 2002;Ohkuchi et al, 2001;Rein et al, 2002). Further studies revealed that the cytokine profile in CD8 + T cells, NK cells and NKT cells also showed the Th1 type-cytokine predominant state (Darmochwal-Kolarz et al, 2002;Borzychowski et al, 2005).…”

Experimental Conditions and Mathematical Analysis of Kinetic Measurements Using Flow Cytometry – The FacsKin Method

“…On contrary to Th1 cells, the activation induced calcium response of the Th2 subset is not decreased compared with the non-pregnant state. The decreased activation of the Th1 subset and the lack of decrease in Th2 cells may partly be responsible for the well established Th2 skewness in healthy pregnancy (Darmochwal-Kolarz et al, 2002;Rein et al, 2002;Saito et al, 1999). Unlike in healthy pregnancy, we could not detect a difference in the AUC values of calcium influx of Th1 and CD8 cells in preeclampsia compared to non-pregnant women.…”

“…The development of a maternal systemic inflammatory response has also been described in PE (Saito et al, 2007). An important feature of this disorder is the absence of Th2 skewness and thus the predominance of pro-inflammatory cytokines, as shown by a number of investigations (Darmochwal-Kolarz et al, 2002;Rein et al, 2002;Saito et al, 1999).…”