Cytokine Expression by Human Macrophage-Like Cells Derived from the Monocytic Cell Line THP-1 Differs between Treatment with Milk from Preterm- and Term-Delivering Mothers and Pasteurized Donor Milk

Demers‐Mathieu, Véronique; Huston, Robert K.; Dallas, David C.

doi:10.3390/molecules25102376

Cited by 2 publications

(1 citation statement)

References 48 publications

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“…A previous study used human milk at a 50 μg/mL concentration to study cytokine expression in THP-1 cells. 16 We tested the dose-response effect of HBM at concentrations of 1-100 μg/mL for CD14, a pattern recognition receptor, induction by RT-PCR, and observed that 20-50 μg/mL was the optimal sub-saturation range without detectable cytotoxicity. Based on these preliminary experiments, further experiments were performed using a 25 μg/mL concentration.…”

Section: Effect Of Human Breast Milk (Hbm) On Macrophage Phagocytosismentioning

confidence: 99%

Effect of human breast milk on innate immune response: Up-regulation of bacterial pattern recognition receptors and innate cytokines in THP-1 monocytic cells

et al. 2021

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Human breast milk (HBM) contains many bioactive components that protect infants from various microorganisms. Pattern recognition receptors on phagocytic cells recognize microbial pathogens and promote the innate immune system. This study aimed to evaluate the effect of HBM on the expression of pattern recognition receptors and innate cytokines in the monocytic cell line THP-1 and the phagocytic activity of RAW264.7 macrophages. Expression levels of specific mRNAs in THP-1 cells were quantitated using reverse transcription-polymerase chain reaction. Phagocytic activity was measured by fluorescence microscopy to detect the uptake of fluorescent dye-labeled carboxylate-modified polystyrene latex beads in RAW264.7 macrophages. HBM stimulated the phagocytic activity of RAW264.7 macrophages. HBM increased mRNA expression of pattern recognition receptors, including the cluster of differentiation 14 and toll-like receptor 2 and 4, and various innate cytokines, including tumor necrosis factor α, interleukin-1β, C-X-C motif chemokine 8, and C-C motif chemokine ligand 2, in THP-1 monocytic cells. Furthermore, milk oligosaccharides in HBM, such as lacto- N-fucopentaose I, enhanced the expression of pattern recognition receptors and various innate cytokines. HBM is able to modulate the innate immune response by upregulating the expression of pattern recognition receptors and various innate cytokines in monocytes/macrophages.

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Section: Effect Of Human Breast Milk (Hbm) On Macrophage Phagocytosismentioning

confidence: 99%

Effect of human breast milk on innate immune response: Up-regulation of bacterial pattern recognition receptors and innate cytokines in THP-1 monocytic cells

et al. 2021

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Peptides Derived from In Vitro and In Vivo Digestion of Human Milk Are Immunomodulatory in THP-1 Human Macrophages

Liang

Beverly

Scottoline

et al. 2022

The Journal of Nutrition

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Background Milk proteins contain many encrypted bioactive peptides. Whether these bioactive peptides are released in the infant intestine and exert immunomodulatory activity remains unknown. Objective This study examined in vitro immunomodulatory activities of peptides from in vitro- and in vivo-digested human milk. Methods Peptides were extracted from in vitro-digested human milk and pooled intestinal samples from eight infants fed human milk. Peptides extracted from in vitro-digested samples were fractionated. The in vitro effects of these peptides and fractions on the secretion of TNFα and IL-8 in LPS-treated human immune THP-1 macrophages were evaluated. The significance of differences between in vitro peptide fraction treatment and control on cytokine production were analyzed by t-test. LC-MS/MS-based peptidomics was conducted to identify the peptides. The peptides were screened for potential bioactivity using a sequence homology search against Milk Bioactive Peptide Database (MBPDB). Results Six fractions of the peptide mixture extracted from the in vitro-digested human milk significantly inhibited TNFα production by LPS-challenged THP-1 macrophages. Fractions F4, F8, F11, F14, and F17 attenuated IL-8 secretion, and F6/7 and F18 increased IL-8 secretion. Peptides extracted from the pooled in vivo intestinal samples attenuated both TNFα and IL-8 secretion. There were 266 and 418 peptides identified in the in vitro and in vivo samples, respectively. Among the peptides, 34 and 50 in the in vitro and in vivo samples, respectively, had > 80% sequence similarity to bioactive peptides in the MBPDB. Conclusions Peptides released by in vitro and in vivo infant digestion of human milk were immunomodulatory in human immune cells, fractions F4, F8, and F11 were anti-inflammatory, and F6/7 and F18 were pro-inflammatory. Thirteen peptides were present in all fractions with anti-inflammatory activity, and 38 peptides were present in all fractions with pro-inflammatory activity. These peptides potentially contributed to the observed immunomodulatory activity of the peptide mixtures.

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Cytokine Expression by Human Macrophage-Like Cells Derived from the Monocytic Cell Line THP-1 Differs between Treatment with Milk from Preterm- and Term-Delivering Mothers and Pasteurized Donor Milk

Cited by 2 publications

References 48 publications

Effect of human breast milk on innate immune response: Up-regulation of bacterial pattern recognition receptors and innate cytokines in THP-1 monocytic cells

Effect of human breast milk on innate immune response: Up-regulation of bacterial pattern recognition receptors and innate cytokines in THP-1 monocytic cells

Peptides Derived from In Vitro and In Vivo Digestion of Human Milk Are Immunomodulatory in THP-1 Human Macrophages

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