Cytokine and chemokine responses in pediatric patients with severe pneumonia associated with pandemic A/H1N1/2009 influenza virus

Matsumoto, Yuji; Kawamura, Yoshiki; Nakai, Hidetaka; Sugata, Ken; Yoshikawa, Akiko; Ihira, Masaru; Ohashi, Masahiro; Kato, Tomochika; Yoshikawa, Tetsushi

doi:10.1111/j.1348-0421.2012.00489.x

Cited by 15 publications

(17 citation statements)

References 14 publications

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“…However in the human cohort the fold increases in cytokine levels was more modest and the temporal cytokine flux was more complex. Although the significant differences in types and clinical characteristics of cohorts reported in literature prevented direct comparison of our results, elevation of inflammatory cytokines and chemokines early during presentation, and the levels we have observed, are consistent with reports in literature [22], [23], [24]. Additionally, some of these differences could also be attributed to different immune response to different viral strains.…”

Section: Discussionsupporting

confidence: 91%

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

et al. 2014

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BackgroundManagement of influenza, a major contributor to the worldwide disease burden, is complicated by lack of reliable methods for early identification of susceptible individuals. Identification of molecular markers that can augment existing diagnostic tools for prediction of severity can be expected to greatly improve disease management capabilities.Methodology/Principal FindingsWe have analyzed cytokines, proteome flux and protein adducts in bronchoalveolar lavage (BAL) and sera from mice infected with influenza A virus (PR8 strain) using a previously established non-lethal model of influenza infection. Through detailed cytokine and protein adduct measurements of murine BAL, we first established the temporal profile of innate and adaptive responses as well as macrophage and neutrophil activities in response to influenza infection. A similar analysis was also performed with sera from a longitudinal cohort of influenza patients. We then used an iTRAQ-based, comparative serum proteome analysis to catalog the proteome flux in the murine BAL during the stages correlating with “peak viremia,” “inflammatory damage,” as well as the “recovery phase.” In addition to activation of acute phase responses, a distinct class of lung proteins including surfactant proteins was found to be depleted from the BAL coincident with their “appearance” in the serum, presumably due to leakage of the protein following loss of the integrity of the lung/epithelial barrier. Serum levels of at least two of these proteins were elevated in influenza patients during the febrile phase of infection compared to healthy controls or to the same patients at convalescence.Conclusions/SignificanceThe findings from this study provide a molecular description of disease progression in a mouse model of influenza and demonstrate its potential for translation into a novel class of markers for measurement of acute lung injury and improved case management.

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Section: Discussionsupporting

confidence: 91%

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

et al. 2014

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“…It has previously been shown that children with a laboratory confirmed influenza A (2009 H1N1) virus pneumonia had higher IL-5 concentrations compared to children without a lower respiratory tract infection [19][20][21]. In influenza A H1N1 virus pneumonia serum IL-5 levels were even higher among those patients requiring mechanical ventilation [19] or in influenza patients without viral pneumonia [21]. In our adult study population, we had only one pneumonic patient with a H1N1 influenza viral infection, while the other pneumonias were due to adeno-, corona-, or rhinovirus infections.…”

Section: Discussionmentioning

confidence: 99%

Interleukin-5, interleukin-6, interferon induced protein-10, procalcitonin and C-reactive protein among mechanically ventilated severe community-acquired viral and bacterial pneumonia patients

et al. 2019

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“…The production of specific cytokines such as the tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6 and IL-10, and chemokine IL-8, are induced by viral infection, as demonstrated by an analysis of the sera of pandemic influenza-infected patients [13]–[15].…”

Section: Introductionmentioning

confidence: 99%

Pregnant Women Infected with Pandemic H1N1pdm2009 Influenza Virus Displayed Overproduction of Peripheral Blood CD69+ Lymphocytes and Increased Levels of Serum Cytokines

et al. 2014

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The first pandemic of the 21st century occurred in 2009 and was caused by the H1N1pdm influenza A virus. Severe cases of H1N1pdm infection in adults are characterized by sustained immune activation, whereas pregnant women are prone to more severe forms of influenza, with increased morbi-mortality. During the H1N1pdm09 pandemic, few studies assessed the immune status of infected pregnant women. The objective of this study was to evaluate the behavior of several immune markers in 13 H1N1pdm2009 virus-infected pregnant (PH1N1) women, in comparison to pregnant women with an influenza-like illness (ILI), healthy pregnant women (HP) and healthy non-pregnant women (HW). The blood leukocyte phenotypes and the serological cytokine and chemokine concentrations of the blood leukocytes, as measured by flow cytometry, showed that the CD69+ cell counts in the T and B-lymphocytes were significantly higher in the PH1N1 group. We found that pro-inflammatory (TNF-α, IL-1β, IL-6) and anti-inflammatory (IL-10) cytokines and some chemokines (CXCL8, CXCL10), which are typically at lower levels during pregnancy, were substantially increased in the women in the ILI group. Our findings suggest that CD69 overexpression in blood lymphocytes and elevated levels of serum cytokines might be potential markers for the discrimination of H1N1 disease from other influenza-like illnesses in pregnant women.

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Cytokine and chemokine responses in pediatric patients with severe pneumonia associated with pandemic A/H1N1/2009 influenza virus

Abstract: ABSTRACT

Cited by 15 publications

References 14 publications

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

Molecular Analysis of Serum and Bronchoalveolar Lavage in a Mouse Model of Influenza Reveals Markers of Disease Severity That Can Be Clinically Useful in Humans

Interleukin-5, interleukin-6, interferon induced protein-10, procalcitonin and C-reactive protein among mechanically ventilated severe community-acquired viral and bacterial pneumonia patients

Pregnant Women Infected with Pandemic H1N1pdm2009 Influenza Virus Displayed Overproduction of Peripheral Blood CD69+ Lymphocytes and Increased Levels of Serum Cytokines

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