Cytokine and Chemokine Induction Using Cell Wall Component and Toxin Derived from Gram-Positive Bacteria in the Rat Middle Ear

Kita, Hidefumi; Himi, Tetsuo

doi:10.1080/00016489950180973

Cited by 12 publications

(1 citation statement)

References 20 publications

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“…Thus, similar to previous studies, IL-10 may contribute to the shift of an acute condition to COME [ 24 , 25 ]. For example, in experimental studies of AOM, overexpression of IL-10 by T-helper lymphocytes (CD4+) in the middle ear lining was observed [ 26 - 28 ]. This overexpression of IL-10 may downregulate the inflammatory response and could switch the condition from the acute to the chronic state.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Interleukin-1 Receptor Antagonist and Immunoregulatory Interleukin-10 in the Middle Ear in Chronic Otitis Media With Effusion in Children With and Without Atopy

Zielnik-Jurkiewicz

Stankiewicz-Szymczak

2016

Clin Exp Otorhinolaryngol

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ObjectivesThe role of pro-inflammatory cytokines in the course of chronic otitis media with effusion (COME) has been documented. However, there are fewer studies on the action of anti-inflammatory cytokines in the middle ear. We sought determine whether there is an association between COME and anti-inflammatory cytokines and whether there are any differences in the cytokine profile in COME children with and without atopy.MethodsEighty-four children were divided into 3 groups: 32 nonatopic children with COME (group NA), 31 atopic children with COME (group A), and 21 children without COME and without atopy (control group C). Specimens from the middle ear were collected and evaluated by enzyme-linked immunosorbent assay for the cytokines interleukin-1 receptor antagonist (IL-1Ra) and immunoregulatory IL-10.ResultsSignificantly higher IL-10 concentrations were found in both nonatopic and atopic children with COME compared to controls. No significant differences in IL-1Ra levels were found between atopic and nonatopic children with COME and the control group.ConclusionWe found no differences in the levels of IL-1Ra in atopic and nonatopic children with COME compared to controls. However, we found elevated IL-10 levels in the middle ear effusions from children with COME, with or without atopy. These elevated immunoregulatory cytokine levels suggest a role for new immunomodulatory treatments to prevent disease progression in COME, regardless of atopy.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Interleukin-1 Receptor Antagonist and Immunoregulatory Interleukin-10 in the Middle Ear in Chronic Otitis Media With Effusion in Children With and Without Atopy

Zielnik-Jurkiewicz

Stankiewicz-Szymczak

2016

Clin Exp Otorhinolaryngol

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Bacterial products increase expression of the human cathelicidin hCAP-18/LL-37 in cultured human sinus epithelial cells

Nell

Tjabringa

Vonk

et al. 2004

FEMS Immunology &Amp; Medical Microbiology

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The respiratory epithelium plays a major role in the primary defense of the airways against infection. It has been demonstrated that bacterial products are involved in the induction of inflammatory reactions of the upper airways. Little is known about the effects of bacterial products on expression of the antimicrobial peptide hCAP-18/LL-37, the only human cathelicidin identified so far. The aim of this study was to investigate the effects of bacterial products from both gram-positive and gram-negative bacteria on the expression of hCAP-18/LL-37 by sinus epithelial cells using an air-exposed tissue culture model. Lipopolysaccharide and lipoteichoic acid both increased hCAP-18/LL-37 expression in cultured sinus epithelium as assessed by immunohistochemistry, where maximal stimulation occurred at 100 ng ml(-1) lipopolysaccharide or 10 microg ml(-1) lipoteichoic acid. The stimulatory effect of lipopolysaccharide and lipoteichoic acid was not restricted to expression of hCAP-18/LL-37, since also mucin expression and IL-8 release from cultured sinus epithelium cells were increased by lipopolysaccharide and lipoteichoic acid. This suggests that bacterial products may stimulate innate immunity in the upper airways.

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Interleukin-8 induction via NF-κB, p38 mitogen-activated protein kinase and extracellular signal-regulated kinase1/2 pathways in human peripheral blood mononuclear cells by Alloiococcus otitidis

Harimaya

Koizumi

Fujii

et al. 2007

International Journal of Pediatric Otorhinolaryngology

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Cytokine and Chemokine Induction Using Cell Wall Component and Toxin Derived from Gram-Positive Bacteria in the Rat Middle Ear

Cited by 12 publications

References 20 publications

Evaluation of the Interleukin-1 Receptor Antagonist and Immunoregulatory Interleukin-10 in the Middle Ear in Chronic Otitis Media With Effusion in Children With and Without Atopy

Evaluation of the Interleukin-1 Receptor Antagonist and Immunoregulatory Interleukin-10 in the Middle Ear in Chronic Otitis Media With Effusion in Children With and Without Atopy

Bacterial products increase expression of the human cathelicidin hCAP-18/LL-37 in cultured human sinus epithelial cells

Interleukin-8 induction via NF-κB, p38 mitogen-activated protein kinase and extracellular signal-regulated kinase1/2 pathways in human peripheral blood mononuclear cells by Alloiococcus otitidis

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