Cytokine and Chemokine Gene Expression of IL‐1β Stimulated Equine Articular Chondrocytes

David, Florent; Farley, Judith; Huang, Huei-Jean; Lavoie, Jean‐Pierre; Laverty, Sheila

doi:10.1111/j.1532-950x.2007.00253.x

Cited by 50 publications

(43 citation statements)

References 35 publications

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“…This could be explained by MDA role in stabilization of collagen via MDA crosslinking that encourages more excessive glycation which start further lipid peroxidation. Moreover, as previously reported by [28] high MDA can induce many inflammatory cytokines. These cytokines trigger others production and lead to more oxidative stress [29].…”

Section: Discussionmentioning

confidence: 61%

Malondialdehyde, Adiponectin, Nitric Oxide, C-Reactive Protein, Tumor Necrosis Factor-Alpha and Insulin Resistance Relationships and Inter-Relationships in Type 2 Diabetes Early Stage Is Metformin Alone Adequate in This Stage

Saad

Habib

Refai

et al. 2017

Int J Pharm Pharm Sci

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Objective: Great interest is directed to inflammation and oxidative stress involvement in type 2 diabetes pathogenesis. Many researchers suggest they play roles but exactly how is still not clear enough. This encouraged us to investigate relations and potential inter-relationships between them and insulin resistance in type 2 diabetes in its early stage. Whether metformin drug alone, as frequently prescribed, is enough for type 2 diabetes management in this early stage was also an objective.Methods: Blood sugar indices, adiponectin (ADIPOQ), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), nitric oxide (NO), C-reactive protein (CRP), liver and kidney function tests and lipid profile were monitored in non-diabetic volunteers, pre-diabetic and newly diagnosed type 2 diabetic patients before and after metformin drug utilization for 5 mo.Results: MDA, inflammation markers and alanine aminotransferase (ALT) were elevated, and blood sugar indices and lipid profile showed pathological alterations in diabetics compared to non-diabetics; changes were worse in type 2 cases. They were improved to different degrees by metformin treatment except for pancreatic β-cells function and ADIPOQ level showed no significant improvements and it couldn't normalize ALT. Conclusion:Results reflected significant relations and inter-relationships between oxidative stress and inflammation markers in type 2 diabetes in its early stage and indicated that metformin may need to be combined with another drug.

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Section: Discussionmentioning

confidence: 61%

Malondialdehyde, Adiponectin, Nitric Oxide, C-Reactive Protein, Tumor Necrosis Factor-Alpha and Insulin Resistance Relationships and Inter-Relationships in Type 2 Diabetes Early Stage Is Metformin Alone Adequate in This Stage

Saad

Habib

Refai

et al. 2017

Int J Pharm Pharm Sci

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“…Recent evidence has shown that OA also has an inflammatory component (Berenbaum, 2013;Konttinen et al, 2012), where matrix fragments can signal through toll-like receptors. Proteolytic degradation of the matrix can also release immobilised cytokines such as interleukin (IL)-1β and IL-6 and tumour necrosis factor (TNF) which in turn further induce cytokine expression and secretion of matrix-degrading enzymes from chondrocytes, thus propagating tissue breakdown (David et al, 2007;Martel-Pelletier, 1999). These effects are largely mediated by nuclear transcription factors such as nuclear factor-kappaB (NF-κB), which has been shown to induce expression of inflammatory cytokines, matrix metalloproteinases (MMPs), cyclooxygenase-2 (COX-2), as well as adhesion molecules (ICAM-1, VCAM-1 and E-selectin) implicating NF-κB in the recruitment of leukocytes (Ke et al, 2007;Miagkov et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Biomimetic sulphated alginate hydrogels suppress IL-1β-induced inflammatory responses in human chondrocytes

Arlov¹,

Öztürk²,

Steinwachs³

et al. 2017

eCM

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Loss of articular cartilage from ageing, injury or degenerative disease is commonly associated with inflammation, causing pain and accelerating degradation of the cartilage matrix. Sulphated glycosaminoglycans (GAGs) are involved in the regulation of immune responses in vivo, and analogous polysaccharides are currently being evaluated for tissue engineering matrices to form a biomimetic environment promoting tissue growth while suppressing inflammatory and catabolic activities. Here, we characterise physical properties of sulphated alginate (S-Alg) gels for use in cartilage engineering scaffolds, and study their anti-inflammatory effects on encapsulated chondrocytes stimulated with IL-1β. Sulphation resulted in decreased storage modulus and increased swelling of alginate gels, whereas mixing highly sulphated alginate with unmodified alginate resulted in improved mechanical properties compared to gels from pure S-Alg. S-Alg gels showed extensive anti-inflammatory and anti-catabolic effects on encapsulated chondrocytes induced by IL-1β. Cytokine-stimulated gene expression of pro-inflammatory markers IL-6, IL-8, COX-2 and aggrecanase ADAMTS-5 were significantly lower in the sulphated gels compared to unmodified alginate gels. Moreover, sulphation of the microenvironment suppressed the protein expression of COX-2 and NF-κB as well as the activation of NF-κB and p38-MAPK. The sulphated alginate matrices were found to interact with IL-1β, and proposed to inhibit inflammatory induction by sequestering cytokines from their receptors. This study shows promising potential for sulphated alginates in biomimetic tissue engineering scaffolds, by reducing cytokine-mediated inflammation and providing a protective microenvironment for encapsulated cells.

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“…Some cytokines can control the release of other cytokines. This event leads to increased oxidative stress in chronic inflammations (Chokkalingam et al, 2013;David et al, 2007;Vlahopoulos et al, 1999).…”

Section: Cytokinesmentioning

confidence: 95%

Impact of host gene polymorphisms on susceptibility to chronic hepatitis B virus infection

Moudi

Heidari

Mahmoudzadeh‐Sagheb

2016

Infection, Genetics and Evolution

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Cytokine and Chemokine Gene Expression of IL‐1β Stimulated Equine Articular Chondrocytes

Abstract: Chondrocytes express proinflammatory cytokines and chemokines capable of modulating a local inflammatory cascade in articular cartilage, which could potentially lead to focal degradation and osteoarthritis.

Cited by 50 publications

References 35 publications

Malondialdehyde, Adiponectin, Nitric Oxide, C-Reactive Protein, Tumor Necrosis Factor-Alpha and Insulin Resistance Relationships and Inter-Relationships in Type 2 Diabetes Early Stage Is Metformin Alone Adequate in This Stage

Malondialdehyde, Adiponectin, Nitric Oxide, C-Reactive Protein, Tumor Necrosis Factor-Alpha and Insulin Resistance Relationships and Inter-Relationships in Type 2 Diabetes Early Stage Is Metformin Alone Adequate in This Stage

Biomimetic sulphated alginate hydrogels suppress IL-1β-induced inflammatory responses in human chondrocytes

Impact of host gene polymorphisms on susceptibility to chronic hepatitis B virus infection

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