Cytokine and antibody subclass responses in the intestinal lymph of sheep during repeated experimental infections with the nematode parasite Trichostrongylus colubriformis

Pernthaner, A.; Cole, Sally; Morrison, Lilian; Green, Richard; Shaw, R.J.; Hein, Wayne R.

doi:10.1016/j.vetimm.2006.08.004

Cited by 56 publications

(42 citation statements)

References 33 publications

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“…The high correlation observed between IL-4 and IFNG confirms that both of these cytokines can be elicited in the same environment during gastrointestinal nematode infection, as also described by Meeusen et al (2005) and by Pernthaner et al (2006). Understanding the factors that lead to polarization and differentiation of Th1 and Th2 responses in gastrointestinal nematode infections in ruminants is complex (LI et al, 2010;ROBINSON et al, 2011).…”

Section: Discussionsupporting

confidence: 66%

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

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Galectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and Gal14) and collectins (SPA and CGN) in sheep. Twelve Corriedale sheep exposed to natural infection with nematodes were divided into two groups: group 1 (G1, n = 7) and group 2 (G2, n = 5), with low and high parasite burdens, respectively, based on fecal egg counts and abomasal parasite counts. The fecal egg counts and abomasal parasite counts were significantly different (p < 0.05) between the groups. Galectin and collectin gene expression was observed in all sheep abomasal samples. However, animals with lower infection levels showed lower expression of the genes Gal14, SPA and CGN (p < 0.05). Expression of lectins was associated with the abomasal H. contortus burden, thus suggesting that these proteins may have a role in controlling of this infection.Keywords: Innate immunity, lectins, helminths, small ruminants, PCR. ResumoColectinas e galectinas são proteínas da família das lectinas que possuem a capacidade de reconhecer padrões moleculares associados aos patógenos. Estudos em bovinos têm demonstrado a alta expressão dessas proteínas durante a infecção por nematoides gastrintestinais. O objetivo deste estudo foi investigar se o nível de infecção de Haemonchus contortus altera a expressão de colectinas (SPA e CGN) e galectinas (Gal11 e Gal14) de ovinos. Doze ovinos da raça Corriedale expostos a infecção natural com nematoides foram separados em dois grupos: grupo 1 (G1, n=7) com menor grau de parasitismo; e grupo 2 (G2, n=5) com maior grau, a partir da contagem do número de parasitos recuperados do abomaso e OPG. A contagem de OPG e de parasitos recuperados do abomaso dos grupos G1 e G2 apresentaram diferença estatística (p<0,05). A expressão dos genes de colectinas e galectina foi observada em todas as amostras de abomaso dos ovinos, porém animais com menor grau de infecção apresentaram menor expressão dos genes de Gal14, SPA e CGN (p<0,05). A expressão de lectinas foi associada ao número de H. contortus encontrados no abomaso de ovinos, indicando um possível papel dessas proteínas no controle da infecção.Palavras-chave: Imunidade inata, lectinas, helmintos, pequenos ruminantes, PCR.

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Section: Discussionsupporting

confidence: 66%

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Souza

Lambert

Nishi

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…Immune cell depletion and cytokine profile studies have shown that resistance to infection is dependent on the induction of the type two (T2) or the “allergic” phenotype response [48–50]. The Th2 immune response is characterised by the differentiation of T cells that produce the cytokines IL-4, IL-5, IL-9, and IL-13, the proliferation and recruitment of effector cells, and eosinophils, mucosal mast cells and globular leukocytes, along with increased mucus secretion and generation of parasite-specific antibodies such as IgA, IgG 1 and IgE [48, 51]. …”

Section: Ideal Characteristics Of Diagnostic Markers For Gin Parasmentioning

confidence: 99%

Current Status for Gastrointestinal Nematode Diagnosis in Small Ruminants: Where Are We and Where Are We Going?

Preston

Sandeman

González

et al. 2014

Journal of Immunology Research

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Gastrointestinal nematode (GIN) parasites pose a significant economic burden particularly in small ruminant production systems. Anthelmintic resistance is a serious concern to the effective control of GIN parasites and has fuelled the focus to design and promote sustainable control of practices of parasite control. Many facets of sustainable GIN parasite control programs rely on the ability to diagnose infection both qualitatively and quantitatively. Diagnostics are required to determine anthelmintic efficacies, for targeted treatment programs and selection of animals for parasite resistant breeding. This review describes much of the research investigated to date to improve the current diagnostic for the above practices which is based on counting the number of parasite eggs in faeces.

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“…Higher levels of IgG1 have been observed in the lymph of sheep infected with T. columbriformis [32], suggestive of a role for this antibody in nematode resistance. Similarly, elevated levels of IgA have been observed in lymph of sheep undergoing T. circumcincta infection [20,31,33], while IgA-assisted suppression of worm fecundity and arrested L4 development has been reported to mediate resistance in T. circumcincta infected sheep [19,21].…”

Section: Introductionmentioning

confidence: 99%

The dynamic influence of the DRB1*1101 allele on the resistance of sheep to experimental Teladorsagia circumcincta infection

Hassan

Good

Hanrahan

et al. 2011

Vet Res

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Suffolk sheep carrying the DRB1*1101 (previously referred to as-DRB1*0203 or G2) allele have been reported to show increased resistance to natural Teladorsagia circumcincta infection compared to non-carriers. The objective of this study was to compare the biochemical and physiological responses of DRB1*1101 carrier and non-carrier twin lambs to an experimental infection with 3 × 104 L3 Teladorsagia circumcincta. The variables studied included worm burden, faecal egg count, abomasal mast cells, IgA, IgE, IgG1 plus IgG2 and haematological parameters at 0, 3, 7, 21 and 35 days post infection (dpi), and duodenal smooth muscle contractility at 0 and 35 dpi. DRB1*1101 carrier lambs had significantly lower worm burden, higher mast cell and plasma platelet counts than the DRB1*1101 non-carriers (P < 0.05). Before infection, the non-carrier lambs exhibited significantly higher mucosal levels of all antibody isotypes measured compared to the carriers; these levels remained relatively stable over the course of infection in the non-carriers while there was a slow build up of these antibodies in the carriers up to day 21 post infection (pi). The DRB1*1101 non-carrier lambs had a significantly higher plasma lymphocyte count, and produced greater duodenal contractile force relative to the carrier lambs (P < 0.05). There was no significant difference between genotypes in the level of plasma eosinophils, monocytes, neutrophils or FEC. This evidence suggests that resistance conferred by DRB1*1101 is acquired rather than innate, depends on worm expulsion rather than fecundity and is dependent on mucosal mast cell proliferation, platelet activation, and IgA and IgE antibody responses.

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Cytokine and antibody subclass responses in the intestinal lymph of sheep during repeated experimental infections with the nematode parasite Trichostrongylus colubriformis

Cited by 56 publications

References 33 publications

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Galectins and collectinis expression are increased in Haemonchus contortus-infected corriedale sheep

Current Status for Gastrointestinal Nematode Diagnosis in Small Ruminants: Where Are We and Where Are We Going?

The dynamic influence of the DRB1*1101 allele on the resistance of sheep to experimental Teladorsagia circumcincta infection

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