Cytokeratin polypeptides expression in different epithelial elements of human salivary glands

Abstract

Immunofluorescent labeling of human salivary glands was carried out with a battery of monoclonal antibodies reactive with specific cytokeratin polypeptides. All the epithelial elements of the glands were positively labelled by a broad-spectrum cytokeratin antibody (KG 8.13) and by antibody Ks 18.18, which reacts with cytokeratin No. 18 exclusively. Labelling of frozen sections with antibody KM 4.62, which is reactive with the 40 Kd (No. 19) cytokeratin, was confined to the ductal system and apparently absent from the acini. Antibody KA-1, reactive with polypeptides 4, 5 and 6 stained both the myoepithelial cells and the basal cells of the large ducts. Antibody KS 8.58, however, reacted with the basal cells exclusively. It is thus proposed that the combined use of the various monoclonal antibodies may provide a most useful probe in studies on epithelial cell diversity in normal salivary glands as well as in pathological disorders of that gland.

Monoclonal antibody to keratin K8.12 expression in ductal basal cells of obstructive lesions and in outer tumor cells of salivary pleomorphic adenomas.

“…The histogenesis of salivary pleomorphic adenoma has been studied extensively by both light and electron microscopy (1,(10)(11)(12)(13). At the present time, two major theories have been proposed as to the progenitor cell of origin responsible for cellular FIG (14,18). In the present study , monoclonal antibody K8.12 (nos.…”

“…The reserve cells located in ductal segments, which specifically reacted for keratin nos. 13 and 16 (K8.12, KS8.55 (14,18)), and LICR-LON-16a (17), might be related to, or coincided with, ductal basal cells, which transform to outer tumor cells. The outer tumor cells of pleomorphic adenomas may originate from ductal basal (reserve) cells, while luminal tumor cells which show an intense staining for PKK1 and KL1, may differentiate from ductal luminal cells.…”

“…Recently, the histogeneses of salivary gland tumors have been evaluated to immunohistochemically detect ductal basal cells as specific staining to keratin proteins (3,8,12,14,18,27,33,34) and many monoclonal antibodies to keratins were decorated to ductal epithelial cells (5-7, 21-23, 31, 32). Preceding papers have reported that pleomorphic adenomas co-expressed keratin and vimentin (6,23,31), and that PKK1 and KL1 decorated luminal tumor cells of tubulo-ductal structures of such benign tumors (21,22).…”

Monoclonal antibodies against keratins bind to intercalated duct and ductal basal cells of normal salivary glands in paraffin sections.

“…Immunohistochemical identification of keratin proteins in ductal basal cells has reported that strong staining for monoclonal antibody LICR-LON-16a was recognized in those cells (reserve cells) (12). Geiger et al (9) noted that positive reactions with monoclonal anti-keratin antibodies KG8. 13 4,5,6), and with KS858 (nos.…”

“…13, 16) existed specifically in ductal basal cells. It has been suggested that basal cells of the salivary gland ducts are the progenitor cells of various tumors, as well as of other proliferative disorders (2,5,6,9,12).…”