Cytokeratin patterns of human oral epithelia: Differences in cytokeratin synthesis in gingival epithelium and the adjacent alveolar mucosa

Oubayoun, Jean Pierre; Gosselin, F.; Forest, Nadine; Winter, Stefanie; Franke, Werner W.

doi:10.1111/j.1432-0436.1985.tb00523.x

Cited by 113 publications

(66 citation statements)

References 29 publications

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“…Cytokeratins are known as differentiation markers of keratinocytes, and the origin of the epithelium regulates their expression (Ouhayoun et al 1985;Haines and Lane 2012). In the present study, both HMK and HaCaT models stained positively for Ck5 and Ck7.…”

Section: Discussionsupporting

confidence: 61%

Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

Gürsoy

Könönen

et al. 2016

Cytotechnology

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In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supported with a semi-solid growth medium, which allows the cells to grow at an airliquid-solid interface from the beginning of the culturing protocol. In this study, the aim was to further develop our previous model to form a multilayered gingival epithelial culture model. Two different epithelial cell lines (HaCaT from skin and HMK from gingiva) were used in all experiments. Both cell lines were grown first as monolayers for 3 days. After that, keratinocytes were trypsinized, counted and seeded on a sterile semi-permeable nitrocellulose membrane placed on the top of a semi-solid growth medium, forming an air-liquid-solid interface for the cells to grow. At days 1, 4, and 7, epithelial cells were fixed, embedded in paraffin, and sectioned for routine Hematoxylin-Eosin staining and immunohistochemistry for cytokeratin (Ck). At day 1, HMK cells grew as monolayers, while HaCaT cells stratified forming an epithelium with two to three layers. At day 4, a stratified epithelium in the HMK model had four to five layers and its proliferation continued up to day 7. HaCaT cells formed a dense and weakly proliferating epithelium with three to four layers of stratification at day 4 but the proliferation disappeared at day 7. At all days, both models were strongly positive for Ck5, Ck7, and Ck 19, and weakly positive for Ck10. Gingival epithelial cells stratify successfully on semi-permeable nitrocellulose membranes, supported with a semi-solid growth medium. This technique allows researchers to construct uniform gingival epithelial cell multilayers at an air-liquid-solid interface, without using collagen gels, resulting in a more reproducible method.

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Section: Discussionsupporting

confidence: 61%

Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

Gürsoy

Könönen

et al. 2016

Cytotechnology

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“…2,8 Their tissue-specific expression is well illustrated by the fact that simple epithelia like liver and pancreas express CKs 8 and 18, 2,9 while all stratified epithelia express CKs 5 and 14. 2,5,10,11 Nonkeratinising stratified epithelia like buccal mucosa and alveolar mucosa express CKs 4 and 13 along with CKs 5 and 14, while keratinising stratified epithelia like tongue, palate and skin express CKs 1 and 10 along with CKs 5 and 14. 2,5,10,11 Because of their differentiation-dependent and tissue-specific expression, CKs are being used as markers in the diagnosis of various epithelial disorders, including cancer.…”

mentioning

confidence: 99%

“…1 These are expressed in a tissue-specific and differentiation-dependent manner. [2][3][4][5][6] Moll et al 2 in 1982 catalogued CKs from different human epithelia according to their molecular weights and isoelectric points and numbered them [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Over the years, 4 more members of the acidic CK family have been detected, increasing the number to 23.…”

mentioning

confidence: 99%

Implications of cytokeratin 8/18 filament formation in stratified epithelial cells: Induction of transformed phenotype

Raul

Sawant

Dange

et al. 2004

Intl Journal of Cancer

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“…Histologically, this epithelium was markedly thickened at embryonic day (E) 18.5 in the Grhl3 -/-animals compared with wild-type (Grhl3 (17,18), whereas the differentiation markers K13, filaggrin, and involucrin were unaltered between Grhl3 -/-and Grhl3 +/+ embryos ( Figure 1, G-L).…”

Section: Effects Of Grhl3 Deletion During Embryogenesis On Oral Epithmentioning

confidence: 98%

“…All the tumors exhibited reduced GRHL3 levels (below 50% of adjacent normal tissue, although in most expression was undetectable), emphasizing the importance of this transcription factor as a driver of HNSCC. Three distinct subsets of equivalent number were identified based on GSK3B and PTEN expression ( Figure 7A): low GSK3B, high PTEN (samples 1-7); low GSK3B, low PTEN (samples 8-16); and normal GSK3B, low PTEN (samples [17][18][19][20][21][22][23]. No association between the tissue of origin of the SCC and GSK3B or PTEN levels was observed.…”

Section: Analysis Of the Grhl3/gsk3b/c-myc Protooncogenic Axis In Hummentioning

confidence: 99%

2809 Identification of a novel proto-oncogenic network in head and neck squamous cell carcinoma

Georgy¹,

Cangkrama²,

Darido³

et al. 2015

European Journal of Cancer

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Background: The developmental transcription factor Grainyhead-like 3 (GRHL3) plays a critical tumor suppressor role in the mammalian epidermis through direct regulation of PTEN and the PI3K/AKT/mTOR signaling pathway. GRHL3 is highly expressed in all tissues derived from the surface ectoderm, including the oral cavity, raising a question about its potential role in suppression of head and neck squamous cell carcinoma (HNSCC).

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Cytokeratin patterns of human oral epithelia: Differences in cytokeratin synthesis in gingival epithelium and the adjacent alveolar mucosa

Cited by 113 publications

References 29 publications

Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

Construction and characterization of a multilayered gingival keratinocyte culture model: the TURK-U model

Implications of cytokeratin 8/18 filament formation in stratified epithelial cells: Induction of transformed phenotype

2809 Identification of a novel proto-oncogenic network in head and neck squamous cell carcinoma

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